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The map label for this gene is mutM

Identifier: 159184256

GI number: 159184256

Start: 314611

End: 315507

Strand: Direct

Name: mutM

Synonym: Atu0321

Alternate gene names: 159184256

Gene position: 314611-315507 (Clockwise)

Preceding gene: 15887667

Following gene: 229007175

Centisome position: 11.07

GC content: 59.98

Gene sequence:

>897_bases
ATGCCAGAATTGCCAGAAGTCGAAACCGTCAGACGAGGCCTCGCACCCGCCATGGAAGGCGCAAGGGTCCGCAAGCTCCA
TCTTGGCCGCCCGGATCTGCGGTTTCCGTTTCCAGCGGATTTCGCGGGGCTGATCGAAGGAAAAACGATCATCTCGCTTG
GGCGCAGGGCGAAATATCTTCTCATCGAGCTGGAGGACGGGCTGACGATCGTGTCCCATCTCGGCATGTCCGGCTCGTTC
CGCATCGAGGCGGAAAAGGGAGAGGGCGGCGAAGCGCCCGGCGCCTTTCATTATGCCCGCTCGAAAGACGGCAAGCATGA
CCATGTCGTCTTTCATCTGGATAAAGGAGAGGAGCGCGTCTGCGTCATCTATAATGATCCGCGCCGCTTCGGCTTCATGC
ATCTGGTGGAGCGCAACAAGCTCGACCTCTATCCCGCCTTTGCCGAGCTTGGGCCCGAACCGACCGGCAATGCGCTGAGC
GCGGATTATCTGGCGAGCCGGTTCGAGGGCAAAAGCCAGCCACTGAAAAGCACGCTGCTGGATCAGAAGATCATTGCCGG
GCTGGGCAATATCTATGTCTGCGAGGCGCTTTGGCGCGCCCATCTTTCTCCCTTGCGGGCGGCGGGAACGCTGGTGACGA
CACGTGGCAAACCGAAAGCGCAGCTCATCGATCTGACGGAGAAAATCCGTGATGTGATCGCCGATGCGATTGCCGCCGGC
GGCTCCTCCCTGCGCGACCATATACAGACGGATGGAACGCTCGGTTATTTCCAGCATTCCTTTTCGGTCTACGATCAGGA
AGGCCAGCCTTGCCGGACACCCGGCTGCGGGGGTACGGTTGAAAGGGTGGTGCAGGCCGGTCGTTCGACATTCTATTGCG
CCGCCTGCCAGAAATAG

Upstream 100 bases:

>100_bases
GCCATGGCAACGCTATCTGTTGCCGCATCGCCTGGGCGCGACATTTCGCGTGCACTGTATAGACCACATGGTCCTAACGA
TAAACTGCCGGATAAGCCAG

Downstream 100 bases:

>100_bases
CGGTGCTGCAAATTACGGAGAAGGGGAGATTGCCGTGGACTATGAAATGATACTGGTCGAGAAGCGCGATGAGGTGGGCG
TCATCACGCTCAACCGCCCC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYLLIELEDGLTIVSHLGMSGSF
RIEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERVCVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALS
ADYLASRFEGKSQPLKSTLLDQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAG
GSSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK

Sequences:

>Translated_298_residues
MPELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYLLIELEDGLTIVSHLGMSGSF
RIEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERVCVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALS
ADYLASRFEGKSQPLKSTLLDQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAG
GSSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK
>Mature_297_residues
PELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYLLIELEDGLTIVSHLGMSGSFR
IEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERVCVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALSA
DYLASRFEGKSQPLKSTLLDQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAGG
SSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=298, Percent_Identity=37.9194630872483, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786932, Length=300, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_AGRT5 (Q8UIH4)

Other databases:

- EMBL:   AE007869
- PIR:   AI2615
- PIR:   H97397
- RefSeq:   NP_353352.2
- ProteinModelPortal:   Q8UIH4
- SMR:   Q8UIH4
- STRING:   Q8UIH4
- GeneID:   1132359
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0321
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- PhylomeDB:   Q8UIH4
- ProtClustDB:   PRK01103
- BioCyc:   ATUM176299-1:ATU0321-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 32662; Mature: 32531

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 288-288 BINDING 106-106 BINDING 128-128 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYL
CCCCCCHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEE
LIELEDGLTIVSHLGMSGSFRIEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERV
EEEECCCEEEHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCEE
CVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALSADYLASRFEGKSQPLKSTLL
EEEECCCCHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
DQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GSSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK
CHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
PELPEVETVRRGLAPAMEGARVRKLHLGRPDLRFPFPADFAGLIEGKTIISLGRRAKYL
CCCCCHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEE
LIELEDGLTIVSHLGMSGSFRIEAEKGEGGEAPGAFHYARSKDGKHDHVVFHLDKGEERV
EEEECCCEEEHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCEE
CVIYNDPRRFGFMHLVERNKLDLYPAFAELGPEPTGNALSADYLASRFEGKSQPLKSTLL
EEEECCCCHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
DQKIIAGLGNIYVCEALWRAHLSPLRAAGTLVTTRGKPKAQLIDLTEKIRDVIADAIAAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GSSLRDHIQTDGTLGYFQHSFSVYDQEGQPCRTPGCGGTVERVVQAGRSTFYCAACQK
CHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194