BacMap

Definition	Agrobacterium tumefaciens str. C58 chromosome circular, complete sequence.
Accession	NC_003062
Length	2,841,580

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The map label for this gene is fadB [H]

Identifier: 229007175

GI number: 229007175

Start: 315542

End: 316315

Strand: Direct

Name: fadB [H]

Synonym: Atu0322

Alternate gene names: 229007175

Gene position: 315542-316315 (Clockwise)

Preceding gene: 159184256

Following gene: 15887673

Centisome position: 11.1

GC content: 61.37

Gene sequence:

>774_bases
GTGGACTATGAAATGATACTGGTCGAGAAGCGCGATGAGGTGGGCGTCATCACGCTCAACCGCCCCAAGGTGCTCAACGC
CCTCAATTCGGTGCTTCTGAAAGAGCTGCGGCACATTCTGTCTTCTTTCTCAGCCGATGATTCCATCGGCGCCATCATCA
TCACCGGCTCGGAAAAGGCATTTGCGGCCGGCGCCGACATCAAGGAAATGCAGGGGCTGGATTTCGTCGATGCCTATGTG
GGCGATTTCCTGGGTGGCTGGGATGAGGTTGCGGCCACACGCAAGCCTGTTATTGCCGCTGTTTCCGGTTTTGCGCTCGG
CGGCGGCTGCGAGCTGGCGATGATGTGCGATTTCATTATCGCTTCGGACACGGCGAAGTTCGGCCAGCCGGAAATCACCC
TCGGCGTCATTCCCGGCATGGGCGGATCGCAGCGGCTGACGCGGGCGGTGGGCAAGGCGAAGGCCATGGACCTCGTTCTG
ACCGGCCGCATGATGGATGCGGCGGAAGCCGAGCGCGCCGGCCTCGTATCGCGTATCGTGCCGGCTGCAGATCTCATGGA
GGTGGCGGTGGAAGCTGCGACCCGCATTGCCTCGCTGTCGCGTGCTTCCGTGCTCATGGCCAAGGAAAGCGTCAACCGCT
CCTTCGAGGTGCCGCTCGCCGAGGGGCTGAGATATGAACGGCGTGCCTTCCAGTCGCTCTTTGCGACGGAGGATCAGAAG
GAGGGCATGGCGGCCTTCGTTGAAAAGCGCAAACCCGCTTTCCGGAATCGCTAA

Upstream 100 bases:

>100_bases
GGTACGGTTGAAAGGGTGGTGCAGGCCGGTCGTTCGACATTCTATTGCGCCGCCTGCCAGAAATAGCGGTGCTGCAAATT
ACGGAGAAGGGGAGATTGCC

Downstream 100 bases:

>100_bases
GGATTCGGAATATCGGCGCTTTTAAGCGTTTTGCGCGTTGACGGGGGTGCGCTTTAGCGGTATATGCCCGCCCACGGTTA
AGGAAAGCCATCCGGCTTGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MDYEMILVEKRDEVGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSEKAFAAGADIKEMQGLDFVDAYV
GDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDFIIASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVL
TGRMMDAAEAERAGLVSRIVPAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATEDQK
EGMAAFVEKRKPAFRNR

Sequences:

>Translated_257_residues
MDYEMILVEKRDEVGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSEKAFAAGADIKEMQGLDFVDAYV
GDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDFIIASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVL
TGRMMDAAEAERAGLVSRIVPAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATEDQK
EGMAAFVEKRKPAFRNR
>Mature_257_residues
MDYEMILVEKRDEVGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSEKAFAAGADIKEMQGLDFVDAYV
GDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDFIIASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVL
TGRMMDAAEAERAGLVSRIVPAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATEDQK
EGMAAFVEKRKPAFRNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=56.7567567567568, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI68989263, Length=229, Percent_Identity=33.6244541484716, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=31.8725099601594, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=35.15625, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=31.3207547169811, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=35.0282485875706, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=32.5102880658436, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI37594471, Length=230, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594469, Length=230, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI25453481, Length=263, Percent_Identity=26.2357414448669, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI51538573, Length=263, Percent_Identity=26.2357414448669, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI4757968, Length=263, Percent_Identity=25.8555133079848, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI49355787, Length=263, Percent_Identity=25.8555133079848, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260274832, Length=228, Percent_Identity=28.5087719298246, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=228, Percent_Identity=28.5087719298246, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=228, Percent_Identity=28.5087719298246, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757966, Length=188, Percent_Identity=30.8510638297872, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=188, Percent_Identity=30.8510638297872, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=255, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=238, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=263, Percent_Identity=25.0950570342205, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=263, Percent_Identity=25.0950570342205, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=263, Percent_Identity=25.0950570342205, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=34.0740740740741, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI62530384, Length=244, Percent_Identity=23.7704918032787, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI157694520, Length=209, Percent_Identity=25.3588516746411, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=44.4444444444444, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=39.2712550607287, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=34.8484848484849, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=34.6733668341709, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=32.0610687022901, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=30.1470588235294, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=30.4347826086957, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=54.0856031128405, Blast_Score=277, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=51.0288065843621, Blast_Score=246, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.920704845815, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=33.8775510204082, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=212, Percent_Identity=33.9622641509434, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=211, Percent_Identity=34.1232227488152, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=211, Percent_Identity=34.1232227488152, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=211, Percent_Identity=34.1232227488152, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=273, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=251, Percent_Identity=32.2709163346614, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=31.1475409836066, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25144160, Length=249, Percent_Identity=31.3253012048193, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=249, Percent_Identity=31.3253012048193, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=27.2030651340996, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=262, Percent_Identity=24.0458015267176, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=30.1980198019802, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=58.1395348837209, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=58.1395348837209, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=34.7169811320755, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=31.7460317460317, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24650670, Length=216, Percent_Identity=34.2592592592593, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=27.906976744186, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=29.4930875576037, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=31.3725490196078, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28571729, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921000, Length=172, Percent_Identity=33.1395348837209, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583077, Length=172, Percent_Identity=33.1395348837209, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583079, Length=172, Percent_Identity=33.1395348837209, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583165, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921018, Length=180, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27625; Mature: 27625

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYEMILVEKRDEVGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSEKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIKEMQGLDFVDAYVGDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDFII
EECCCCHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCEEECCCCHHHHHHHHHH
ASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVLTGRMMDAAEAERAGLVSRIV
HCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHC
PAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATEDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCHH
EGMAAFVEKRKPAFRNR
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYEMILVEKRDEVGVITLNRPKVLNALNSVLLKELRHILSSFSADDSIGAIIITGSEKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIKEMQGLDFVDAYVGDFLGGWDEVAATRKPVIAAVSGFALGGGCELAMMCDFII
EECCCCHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCEEECCCCHHHHHHHHHH
ASDTAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLVLTGRMMDAAEAERAGLVSRIV
HCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHC
PAADLMEVAVEAATRIASLSRASVLMAKESVNRSFEVPLAEGLRYERRAFQSLFATEDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCHH
EGMAAFVEKRKPAFRNR
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]