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The map label for this gene is ubiE

Identifier: 159184255

GI number: 159184255

Start: 313691

End: 314467

Strand: Reverse

Name: ubiE

Synonym: Atu0320

Alternate gene names: 159184255

Gene position: 314467-313691 (Counterclockwise)

Preceding gene: 159184259

Following gene: 15887669

Centisome position: 11.07

GC content: 58.04

Gene sequence:

>777_bases
ATGACTGACGCCCGTACGACCGCAGAAGGTGGCATGGAAACGTCCTATGGCTTTCACAAGGTGGATGAAGGCAAGAAGCA
GGGGCTGGTCAATGAAGTCTTCCATAAGGTGGCCAAGCGCTACGACATTATGAATGACGTGATGTCGGCCGGCCTGCACC
GGCTGTGGAAGGACGCCATGGTCTCCGCGCTTTCGCCGCGCAAGGATCCTTCCTACAAGATTCTGGACGTGGCGGGCGGC
ACTGGCGATGTCGCCTTCCGTATCATCGAAGCCTCCAACCGTCTGGCGCATGCCACCGTTCTCGATATCAACGGCTCGAT
GCTGGCTGTGGGCGAGGAACGCGCTGCGAAAAAGAAGCTTTCCGACAATCTGACCTTCGTCGAGGCCAATGCCGAGGAGC
TGCCCTTCGAGGCGAATACATTCGACGCCTATACGGTCGCCTTCGGCATTCGCAATGTGCCGCGCATCGATGTGGCGCTG
AAGGAGGCCTACCGCGTGCTGAAACGCGGCGGACGGCTGCTGGTGCTGGAATTTTCCGAAGTCGACATGCCGCTGCTCGA
CCGCGTCTATGATGCCTGGTCGTTCAACGCCATTCCGCAATTCGGCAAGATGATCACCGGCGATGCCGAGCCCTATCAAT
ATCTGGTCGAGTCGATCCGCAAATTCCCCAATCAGGAGGATTTTGCAACGATGATCCGTACTGCCGGCTTTTCCCGCGTC
ACCTATACCAATTATACCGGCGGCATCGCCGCCCTGCATTCCGGCTGGAAGCTTTAA

Upstream 100 bases:

>100_bases
CAGTGCACGCGAAATGTCGCGCCCAGGCGATGCGGCAACAGATAGCGTTGCCATGGCGTTTCCGCTATGGTCCGCCACGC
AATAAATATGGAGACAGATG

Downstream 100 bases:

>100_bases
GACCATGCCCGGCAGACGCAGGAGACAAGCATAAGCATGAGTACTTTGGGCGCATATTTCCGCCTCGCTAGGGTTGGCTG
GGTTCTCGTGCGCGAAGGCG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAMVSALSPRKDPSYKILDVAGG
TGDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKLSDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVAL
KEAYRVLKRGGRLLVLEFSEVDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRV
TYTNYTGGIAALHSGWKL

Sequences:

>Translated_258_residues
MTDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAMVSALSPRKDPSYKILDVAGG
TGDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKLSDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVAL
KEAYRVLKRGGRLLVLEFSEVDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRV
TYTNYTGGIAALHSGWKL
>Mature_257_residues
TDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAMVSALSPRKDPSYKILDVAGGT
GDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKLSDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVALK
EAYRVLKRGGRLLVLEFSEVDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRVT
YTNYTGGIAALHSGWKL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=279, Percent_Identity=48.0286738351254, Blast_Score=260, Evalue=1e-69,
Organism=Escherichia coli, GI48994983, Length=245, Percent_Identity=45.3061224489796, Blast_Score=227, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI17557039, Length=252, Percent_Identity=51.5873015873016, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6323526, Length=258, Percent_Identity=44.1860465116279, Blast_Score=202, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24641702, Length=258, Percent_Identity=48.4496124031008, Blast_Score=247, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_AGRT5 (Q8UIH5)

Other databases:

- EMBL:   AE007869
- PIR:   AH2615
- PIR:   G97397
- RefSeq:   NP_353351.2
- ProteinModelPortal:   Q8UIH5
- STRING:   Q8UIH5
- GeneID:   1132358
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0320
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   Q8UIH5
- ProtClustDB:   PRK00216
- BioCyc:   ATUM176299-1:ATU0320-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28731; Mature: 28600

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAM
CCCCCCCCCCCCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSALSPRKDPSYKILDVAGGTGDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKL
HHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCEEEECHHHHHHHHH
SDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVALKEAYRVLKRGGRLLVLEFSE
HCCCEEEECCHHHCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
VDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRV
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
TYTNYTGGIAALHSGWKL
EEECCCCCHHHHHCCCCC
>Mature Secondary Structure 
TDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAM
CCCCCCCCCCCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSALSPRKDPSYKILDVAGGTGDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKL
HHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCEEEECHHHHHHHHH
SDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVALKEAYRVLKRGGRLLVLEFSE
HCCCEEEECCHHHCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
VDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRV
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
TYTNYTGGIAALHSGWKL
EEECCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194