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The map label for this gene is hemN [C]

Identifier: 159184259

GI number: 159184259

Start: 319179

End: 320378

Strand: Reverse

Name: hemN [C]

Synonym: Atu0325

Alternate gene names: 159184259

Gene position: 320378-319179 (Counterclockwise)

Preceding gene: 159184260

Following gene: 159184255

Centisome position: 11.27

GC content: 61.33

Gene sequence:

>1200_bases
ATGGTCGGGGAGCACCAACTTTCTGCCCCCGGCGCATCGCTTCTGCCGGATACGGGCGATCCCGGTTTCGGGATCTATCT
GCACTGGCCCTTCTGCGCGGCCAAATGCCCCTATTGCGATTTCAACAGCCATGTCCGCCACCGGCCGGTGGATCAGGAAC
GGTTTACGGCGGCTTTCCTGCGTGAAATGGAACATATGCGGACGCTGAGCGGCCCGCGCGTCGTCACCAGCATTTTCATG
GGCGGCGGTACGCCTTCGCTGATGGACCCACAGACGGTCGGTGCGCTACTGGATGGCATCTCGCGTTTCTGGCATGTGCC
TGATGGTATCGAGATCACCATGGAGGCTAATCCTTCCAGCGTCGAGGCAGAACGGTTTCGGGGTTATCGGGCGGCAGGCG
TCAATCGTGTCTCGCTCGGCGTGCAGGCACTGAATGACCGCGACCTGAAATTCCTCGGCCGCCTGCATGATGTCGCCGAT
GCCTTGAAAGCCATTCGGTTGGCGCGGGAGATTTTTCCGCGTATGTCCTTCGATCTCATCTATGCCCGCCCGAACCAGAC
GGTGGCCGAATGGGACGCCGAACTGAAGGAGGCTGTCTCCTATGCCGTCGACCATCTGTCGCTCTATCAGCTGACGATCG
AGGAAGGCACACCCTTTTATGGCCTGCACAAGGCCGGCAAGCTCATCGTTCCGGATGGCGAACATTCGGCCGTGCTTTAT
GAGGCGACGCAGGAAATCACCGAGCGTTACGGCATGCCGGCCTATGAGGTTTCCAATCACGCCCGGCCCGGCGCCGAAAG
CCGCCATAACCTCACCTACTGGCGATATGGCGATTATGCCGGCATCGGCCCCGGTGCGCATGGACGCCTGACGCGTGGCG
CTTCCAAGCTCGCCACCGCTACCGAGCGGCACCCCGAGACCTGGCTCGAAACCGTTGAGCGGGAAGGCCATGGCATGGTC
GACCAGGAATTGCTCGGTGTCGATGAACAGGCTGATGAATTGCTGCTGATGGGCCTTCGCCTGCGCGAAGGCATCGATCT
CGCCCGTTGGAGCGATCTCTCCGGCCGCGACCTCGACCCGGAAAAGGAGGAGTTTCTCCTTCAGCACGGTTTCGTGGAAA
GGCTCGGCAATTCCCGCCTGCGCTGCACACCCTCCGGCATGCTTATTCTGGATGCCGTGGTGGCCGATCTCGCCTGCTGA

Upstream 100 bases:

>100_bases
ATGAAAAACACGGCTGGAAACATGGCGACGCTTTCGCACTTTCTCACCGCGCCCGGGCGTTCAAAAAATTCGTAGAAACC
TGCCTGGAGGCATGAACCCG

Downstream 100 bases:

>100_bases
GGCCAGCGCCACCCGGCTGAACCCGATAACGAGAATTCAATAAAAAAGCGGCCCGAAGGCCGCTTTTTTTCATGCGTTGT
TTTCGTTGATCAGGCGTTTC

Product: coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MVGEHQLSAPGASLLPDTGDPGFGIYLHWPFCAAKCPYCDFNSHVRHRPVDQERFTAAFLREMEHMRTLSGPRVVTSIFM
GGGTPSLMDPQTVGALLDGISRFWHVPDGIEITMEANPSSVEAERFRGYRAAGVNRVSLGVQALNDRDLKFLGRLHDVAD
ALKAIRLAREIFPRMSFDLIYARPNQTVAEWDAELKEAVSYAVDHLSLYQLTIEEGTPFYGLHKAGKLIVPDGEHSAVLY
EATQEITERYGMPAYEVSNHARPGAESRHNLTYWRYGDYAGIGPGAHGRLTRGASKLATATERHPETWLETVEREGHGMV
DQELLGVDEQADELLLMGLRLREGIDLARWSDLSGRDLDPEKEEFLLQHGFVERLGNSRLRCTPSGMLILDAVVADLAC

Sequences:

>Translated_399_residues
MVGEHQLSAPGASLLPDTGDPGFGIYLHWPFCAAKCPYCDFNSHVRHRPVDQERFTAAFLREMEHMRTLSGPRVVTSIFM
GGGTPSLMDPQTVGALLDGISRFWHVPDGIEITMEANPSSVEAERFRGYRAAGVNRVSLGVQALNDRDLKFLGRLHDVAD
ALKAIRLAREIFPRMSFDLIYARPNQTVAEWDAELKEAVSYAVDHLSLYQLTIEEGTPFYGLHKAGKLIVPDGEHSAVLY
EATQEITERYGMPAYEVSNHARPGAESRHNLTYWRYGDYAGIGPGAHGRLTRGASKLATATERHPETWLETVEREGHGMV
DQELLGVDEQADELLLMGLRLREGIDLARWSDLSGRDLDPEKEEFLLQHGFVERLGNSRLRCTPSGMLILDAVVADLAC
>Mature_399_residues
MVGEHQLSAPGASLLPDTGDPGFGIYLHWPFCAAKCPYCDFNSHVRHRPVDQERFTAAFLREMEHMRTLSGPRVVTSIFM
GGGTPSLMDPQTVGALLDGISRFWHVPDGIEITMEANPSSVEAERFRGYRAAGVNRVSLGVQALNDRDLKFLGRLHDVAD
ALKAIRLAREIFPRMSFDLIYARPNQTVAEWDAELKEAVSYAVDHLSLYQLTIEEGTPFYGLHKAGKLIVPDGEHSAVLY
EATQEITERYGMPAYEVSNHARPGAESRHNLTYWRYGDYAGIGPGAHGRLTRGASKLATATERHPETWLETVEREGHGMV
DQELLGVDEQADELLLMGLRLREGIDLARWSDLSGRDLDPEKEEFLLQHGFVERLGNSRLRCTPSGMLILDAVVADLAC

Specific function: Unknown

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=390, Percent_Identity=38.4615384615385, Blast_Score=254, Evalue=8e-68,
Organism=Escherichia coli, GI1789325, Length=379, Percent_Identity=34.5646437994723, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082341, Length=308, Percent_Identity=24.3506493506494, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 44412; Mature: 44412

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGEHQLSAPGASLLPDTGDPGFGIYLHWPFCAAKCPYCDFNSHVRHRPVDQERFTAAFL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
REMEHMRTLSGPRVVTSIFMGGGTPSLMDPQTVGALLDGISRFWHVPDGIEITMEANPSS
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
VEAERFRGYRAAGVNRVSLGVQALNDRDLKFLGRLHDVADALKAIRLAREIFPRMSFDLI
CCHHHHCCHHHCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YARPNQTVAEWDAELKEAVSYAVDHLSLYQLTIEEGTPFYGLHKAGKLIVPDGEHSAVLY
EECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEHHCCCEEEECCCCCCEEHH
EATQEITERYGMPAYEVSNHARPGAESRHNLTYWRYGDYAGIGPGAHGRLTRGASKLATA
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCHHHHHHH
TERHPETWLETVEREGHGMVDQELLGVDEQADELLLMGLRLREGIDLARWSDLSGRDLDP
HHCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
EKEEFLLQHGFVERLGNSRLRCTPSGMLILDAVVADLAC
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVGEHQLSAPGASLLPDTGDPGFGIYLHWPFCAAKCPYCDFNSHVRHRPVDQERFTAAFL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
REMEHMRTLSGPRVVTSIFMGGGTPSLMDPQTVGALLDGISRFWHVPDGIEITMEANPSS
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
VEAERFRGYRAAGVNRVSLGVQALNDRDLKFLGRLHDVADALKAIRLAREIFPRMSFDLI
CCHHHHCCHHHCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YARPNQTVAEWDAELKEAVSYAVDHLSLYQLTIEEGTPFYGLHKAGKLIVPDGEHSAVLY
EECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEHHCCCEEEECCCCCCEEHH
EATQEITERYGMPAYEVSNHARPGAESRHNLTYWRYGDYAGIGPGAHGRLTRGASKLATA
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCHHHHHHH
TERHPETWLETVEREGHGMVDQELLGVDEQADELLLMGLRLREGIDLARWSDLSGRDLDP
HHCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
EKEEFLLQHGFVERLGNSRLRCTPSGMLILDAVVADLAC
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]