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The map label for this gene is aarF [H]

Identifier: 15887669

GI number: 15887669

Start: 312080

End: 313654

Strand: Reverse

Name: aarF [H]

Synonym: Atu0319

Alternate gene names: 15887669

Gene position: 313654-312080 (Counterclockwise)

Preceding gene: 159184255

Following gene: 159184254

Centisome position: 11.04

GC content: 61.14

Gene sequence:

>1575_bases
ATGAGTACTTTGGGCGCATATTTCCGCCTCGCTAGGGTTGGCTGGGTTCTCGTGCGCGAAGGCGTCGTGCTGGCTTTGCC
GTCCGATGACCTGCCTGCCCCGGCACAATTGTTGAAAGCCGCGCTGAAACCTTTCGCCCGCAGCAAGGCAAAACGGGCGC
AGCGCAGCGACCGGCTAGCCGTGGCGGTCGAGCGGCTTGGGCCTTCCTATGTGAAGATGGGCCAGTTTCTGGCCACCCGG
CCGGATGTGGTGGGCGCCGATTTCGCCGACGATCTTGCGAGCCTTCAGGACCGCATGGCTTTTTTCCCGGCCGCTGCGGC
GAAGGCCAATATCGAAGGTTCGCTCGGACGATCAATTTCGGAGCTTTATAGAGAATTCGGCGACCCGATCGCCGCCGCCT
CCATCGCCCAGGTGCACCCGGCCATGGTGGATACACCAAAGGGACCGCGCAAGGTGGCCGTCAAGGTCATTCGACCCGGC
GTGCGTCAGCGTTTCCAGAACGATCTCGAGGCGATGTATCTGATTGCCGACCTGCAGCAGCGTTTCGTTCGCTCCGCCCG
TCGCCTGCGGCCGGTGGAAGTGACACGCACGCTGGAGCAGACGACCAAGATCGAGATGGATCTGCGGCTCGAGGCCGCCG
CACTTTCCGAACTTGCGGAAAATACCAGACAAGATCCCGGCTTCCGTGTTCCCGAAGTGGATTGGGAACGCACCGGCCGC
GACGTCGTCACCATGGAATGGATCGACGGCGTCAAGATGTCGGATATCGAGGGACTGAAGGCAGCCGGCCACGACCTGAA
CAAGCTGGCCGATACGCTGATCCAGTCCTTCCTGCGGCATACGCTGCGCGACGGTTTTTTCCATGCCGACATGCATCCCG
GCAACCTCTTCGTCGATGCGAAGGGCGAGATCGTCGCCGTGGATATGGGCATTGCCGGTCGGCTCGGCAAAAAGGAACGC
CGCTTCCTTGCCGAAATCCTCTATGGCTTCATCACGCGTGACTACATGCGGGTGGCGGAAGTGCATTTCGAGGCCGGTTA
CGTGCCGGGCCATCACGACAAGGCCAGCTTCGCCCAGGCGATCCGCGCCATTGGCGAACCAATCCACGGCCAGCCGGCCG
AGACGATCTCGATGGGCAAGCTCCTGACTCTGCTGTTCGAAGTGACCGAGCTTTTCGACATGGAAACGCGGCCGGAACTG
GTGATGCTGCAAAAGACCATGGTGGTGGTGGAAGGCGTGTCGCGCATGCTCAATCCGCGCTTCAATATGTGGAAGGCGGC
CGATCCGGTCGTCGGCGGCTGGATCCGTGACAATCTCGGTCCCAAACGCATCGCCACCGATCTGAAGGACGGCGTGAAGG
CGGCACTCAAGCTCGCCGAAGCAGTGCCCGAGATCGCCGCCAAGACCGAGAAGCTGCATTCCGAACTGATGTATATGAGC
GAAAACGGCCTGCGTTTTGACGCGCAGACGGCGGAAGCCATCGGCAAAGCGGAAGCGCGCCATACCAAATGGGGGCGGAT
TGCGCTCTGGGTGATTGCGCTGACGCTTCTCTATATTGCCATCCGAATCAGCTAA

Upstream 100 bases:

>100_bases
CCGCGTCACCTATACCAATTATACCGGCGGCATCGCCGCCCTGCATTCCGGCTGGAAGCTTTAAGACCATGCCCGGCAGA
CGCAGGAGACAAGCATAAGC

Downstream 100 bases:

>100_bases
GAACGTGAAGGTGCCCGGCGTGACCGCCAGGCGCCTCAGTTATCAAAAAAACCGCCTATTAAGCAGACGTAACGACCGCA
TGCCATGCTCATGCAATATA

Product: ubiquinone biosynthesis protein

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSTLGAYFRLARVGWVLVREGVVLALPSDDLPAPAQLLKAALKPFARSKAKRAQRSDRLAVAVERLGPSYVKMGQFLATR
PDVVGADFADDLASLQDRMAFFPAAAAKANIEGSLGRSISELYREFGDPIAAASIAQVHPAMVDTPKGPRKVAVKVIRPG
VRQRFQNDLEAMYLIADLQQRFVRSARRLRPVEVTRTLEQTTKIEMDLRLEAAALSELAENTRQDPGFRVPEVDWERTGR
DVVTMEWIDGVKMSDIEGLKAAGHDLNKLADTLIQSFLRHTLRDGFFHADMHPGNLFVDAKGEIVAVDMGIAGRLGKKER
RFLAEILYGFITRDYMRVAEVHFEAGYVPGHHDKASFAQAIRAIGEPIHGQPAETISMGKLLTLLFEVTELFDMETRPEL
VMLQKTMVVVEGVSRMLNPRFNMWKAADPVVGGWIRDNLGPKRIATDLKDGVKAALKLAEAVPEIAAKTEKLHSELMYMS
ENGLRFDAQTAEAIGKAEARHTKWGRIALWVIALTLLYIAIRIS

Sequences:

>Translated_524_residues
MSTLGAYFRLARVGWVLVREGVVLALPSDDLPAPAQLLKAALKPFARSKAKRAQRSDRLAVAVERLGPSYVKMGQFLATR
PDVVGADFADDLASLQDRMAFFPAAAAKANIEGSLGRSISELYREFGDPIAAASIAQVHPAMVDTPKGPRKVAVKVIRPG
VRQRFQNDLEAMYLIADLQQRFVRSARRLRPVEVTRTLEQTTKIEMDLRLEAAALSELAENTRQDPGFRVPEVDWERTGR
DVVTMEWIDGVKMSDIEGLKAAGHDLNKLADTLIQSFLRHTLRDGFFHADMHPGNLFVDAKGEIVAVDMGIAGRLGKKER
RFLAEILYGFITRDYMRVAEVHFEAGYVPGHHDKASFAQAIRAIGEPIHGQPAETISMGKLLTLLFEVTELFDMETRPEL
VMLQKTMVVVEGVSRMLNPRFNMWKAADPVVGGWIRDNLGPKRIATDLKDGVKAALKLAEAVPEIAAKTEKLHSELMYMS
ENGLRFDAQTAEAIGKAEARHTKWGRIALWVIALTLLYIAIRIS
>Mature_523_residues
STLGAYFRLARVGWVLVREGVVLALPSDDLPAPAQLLKAALKPFARSKAKRAQRSDRLAVAVERLGPSYVKMGQFLATRP
DVVGADFADDLASLQDRMAFFPAAAAKANIEGSLGRSISELYREFGDPIAAASIAQVHPAMVDTPKGPRKVAVKVIRPGV
RQRFQNDLEAMYLIADLQQRFVRSARRLRPVEVTRTLEQTTKIEMDLRLEAAALSELAENTRQDPGFRVPEVDWERTGRD
VVTMEWIDGVKMSDIEGLKAAGHDLNKLADTLIQSFLRHTLRDGFFHADMHPGNLFVDAKGEIVAVDMGIAGRLGKKERR
FLAEILYGFITRDYMRVAEVHFEAGYVPGHHDKASFAQAIRAIGEPIHGQPAETISMGKLLTLLFEVTELFDMETRPELV
MLQKTMVVVEGVSRMLNPRFNMWKAADPVVGGWIRDNLGPKRIATDLKDGVKAALKLAEAVPEIAAKTEKLHSELMYMSE
NGLRFDAQTAEAIGKAEARHTKWGRIALWVIALTLLYIAIRIS

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI41393593, Length=273, Percent_Identity=30.4029304029304, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40254938, Length=282, Percent_Identity=26.5957446808511, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI217035081, Length=236, Percent_Identity=28.3898305084746, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI34147522, Length=303, Percent_Identity=24.0924092409241, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI217416386, Length=248, Percent_Identity=27.0161290322581, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI27363457, Length=248, Percent_Identity=27.0161290322581, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI2367309, Length=513, Percent_Identity=35.0877192982456, Blast_Score=292, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17559152, Length=325, Percent_Identity=24.3076923076923, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325148, Length=444, Percent_Identity=23.1981981981982, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321319, Length=324, Percent_Identity=24.0740740740741, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323282, Length=291, Percent_Identity=24.0549828178694, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24662575, Length=299, Percent_Identity=29.4314381270903, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI22024280, Length=256, Percent_Identity=26.5625, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 58214; Mature: 58083

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGAYFRLARVGWVLVREGVVLALPSDDLPAPAQLLKAALKPFARSKAKRAQRSDRLA
CCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVERLGPSYVKMGQFLATRPDVVGADFADDLASLQDRMAFFPAAAAKANIEGSLGRSIS
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ELYREFGDPIAAASIAQVHPAMVDTPKGPRKVAVKVIRPGVRQRFQNDLEAMYLIADLQQ
HHHHHHCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFVRSARRLRPVEVTRTLEQTTKIEMDLRLEAAALSELAENTRQDPGFRVPEVDWERTGR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
DVVTMEWIDGVKMSDIEGLKAAGHDLNKLADTLIQSFLRHTLRDGFFHADMHPGNLFVDA
CEEEEHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEC
KGEIVAVDMGIAGRLGKKERRFLAEILYGFITRDYMRVAEVHFEAGYVPGHHDKASFAQA
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRAIGEPIHGQPAETISMGKLLTLLFEVTELFDMETRPELVMLQKTMVVVEGVSRMLNPR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
FNMWKAADPVVGGWIRDNLGPKRIATDLKDGVKAALKLAEAVPEIAAKTEKLHSELMYMS
CCCHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENGLRFDAQTAEAIGKAEARHTKWGRIALWVIALTLLYIAIRIS
CCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLGAYFRLARVGWVLVREGVVLALPSDDLPAPAQLLKAALKPFARSKAKRAQRSDRLA
CCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVERLGPSYVKMGQFLATRPDVVGADFADDLASLQDRMAFFPAAAAKANIEGSLGRSIS
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ELYREFGDPIAAASIAQVHPAMVDTPKGPRKVAVKVIRPGVRQRFQNDLEAMYLIADLQQ
HHHHHHCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFVRSARRLRPVEVTRTLEQTTKIEMDLRLEAAALSELAENTRQDPGFRVPEVDWERTGR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
DVVTMEWIDGVKMSDIEGLKAAGHDLNKLADTLIQSFLRHTLRDGFFHADMHPGNLFVDA
CEEEEHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEC
KGEIVAVDMGIAGRLGKKERRFLAEILYGFITRDYMRVAEVHFEAGYVPGHHDKASFAQA
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRAIGEPIHGQPAETISMGKLLTLLFEVTELFDMETRPELVMLQKTMVVVEGVSRMLNPR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
FNMWKAADPVVGGWIRDNLGPKRIATDLKDGVKAALKLAEAVPEIAAKTEKLHSELMYMS
CCCHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ENGLRFDAQTAEAIGKAEARHTKWGRIALWVIALTLLYIAIRIS
CCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA