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The map label for this gene is hisH

Identifier: 159184136

GI number: 159184136

Start: 42636

End: 43286

Strand: Reverse

Name: hisH

Synonym: Atu0041

Alternate gene names: 159184136

Gene position: 43286-42636 (Counterclockwise)

Preceding gene: 15887400

Following gene: 15887398

Centisome position: 1.52

GC content: 60.98

Gene sequence:

>651_bases
ATGCGTGTCGCGATTATCGACTACGGTTCGGGCAATTTGCGTTCGGCCACCAAAGCTTTCGAGCGTGCCGCCCGCGAGGC
TGGCATCGACGCAACCATCGATCTGACCGACAAGCCGGATCACGTCGCTTCGGCGGACCGCATCGTCCTGCCGGGCGTCG
GTGCCTATGCGGATTGCAGTGCGGGTCTGAATGCCATGCCCGGTATGCACGAGGCGCTCATCGAAGCCGTCGAGAAAAAA
GCCCATCCTTTCCTCGGCATCTGCGTCGGCATGCAGCTGATGTCCTCGCGCGGACTGGAAAAGACCGTCAGCACCGGGCT
CGGCTGGATCGAAGGTGACGTCGTGGAAATGACGCCGTCCGATCCCACTCTTAAAATCCCGCAGATCGGCTGGAATACGC
TGGAAATCCGCCACGCGCACCCGCTGTTCGACGGCATAAAGACCGGTGCGGATGGGCTGCACGCCTATTTCGTGCATTCC
TATCATCTGGCGGCCAAGCACTCGACTGACGTCATCGCCACCACCAATTATGGTGGCGCGATGACGGCTTTCGTCGGCCG
CGACAACATGGCGGGCGCGCAGTTCCACCCGGAAAAAAGCCAGACGCTCGGCCTTGCCCTGATTTCCAATTTCCTGCGCT
GGAAGCCCTGA

Upstream 100 bases:

>100_bases
CGGCCGGTCTGGACCACTTCTGCGCAAAAGAGCGGTGGCAAAAACTTCGTCGACGATCCCGCCGGATCGTTTCTTTTCGA
CTTGAATGGAAGGCGCTGAC

Downstream 100 bases:

>100_bases
CATGATTCTTTTTCCCGCAATCGATCTCAAAGACGGTGAGTGCGTTCGCCTGAAACTCGGCGATATGGAGCAGGCAACCG
TCTATAACGAGGACCCCGGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDATIDLTDKPDHVASADRIVLPGVGAYADCSAGLNAMPGMHEALIEAVEKK
AHPFLGICVGMQLMSSRGLEKTVSTGLGWIEGDVVEMTPSDPTLKIPQIGWNTLEIRHAHPLFDGIKTGADGLHAYFVHS
YHLAAKHSTDVIATTNYGGAMTAFVGRDNMAGAQFHPEKSQTLGLALISNFLRWKP

Sequences:

>Translated_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDATIDLTDKPDHVASADRIVLPGVGAYADCSAGLNAMPGMHEALIEAVEKK
AHPFLGICVGMQLMSSRGLEKTVSTGLGWIEGDVVEMTPSDPTLKIPQIGWNTLEIRHAHPLFDGIKTGADGLHAYFVHS
YHLAAKHSTDVIATTNYGGAMTAFVGRDNMAGAQFHPEKSQTLGLALISNFLRWKP
>Mature_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDATIDLTDKPDHVASADRIVLPGVGAYADCSAGLNAMPGMHEALIEAVEKK
AHPFLGICVGMQLMSSRGLEKTVSTGLGWIEGDVVEMTPSDPTLKIPQIGWNTLEIRHAHPLFDGIKTGADGLHAYFVHS
YHLAAKHSTDVIATTNYGGAMTAFVGRDNMAGAQFHPEKSQTLGLALISNFLRWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=38.4976525821596, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=224, Percent_Identity=32.5892857142857, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_AGRT5 (P58788)

Other databases:

- EMBL:   AE007869
- PIR:   AB2582
- PIR:   H97363
- RefSeq:   NP_353080.2
- ProteinModelPortal:   P58788
- SMR:   P58788
- STRING:   P58788
- GeneID:   1132079
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0041
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   P58788
- ProtClustDB:   PRK13146
- BioCyc:   ATUM176299-1:ATU0041-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23139; Mature: 23139

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 196-196 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGIDATIDLTDKPDHVASADRIVLPGVGAYADCS
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCC
AGLNAMPGMHEALIEAVEKKAHPFLGICVGMQLMSSRGLEKTVSTGLGWIEGDVVEMTPS
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCEECCCEEEECCC
DPTLKIPQIGWNTLEIRHAHPLFDGIKTGADGLHAYFVHSYHLAAKHSTDVIATTNYGGA
CCCEECCCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEHHEEEEECCCCCEEEEECCCCE
MTAFVGRDNMAGAQFHPEKSQTLGLALISNFLRWKP
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGIDATIDLTDKPDHVASADRIVLPGVGAYADCS
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCC
AGLNAMPGMHEALIEAVEKKAHPFLGICVGMQLMSSRGLEKTVSTGLGWIEGDVVEMTPS
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCEECCCEEEECCC
DPTLKIPQIGWNTLEIRHAHPLFDGIKTGADGLHAYFVHSYHLAAKHSTDVIATTNYGGA
CCCEECCCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEHHEEEEECCCCCEEEEECCCCE
MTAFVGRDNMAGAQFHPEKSQTLGLALISNFLRWKP
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194