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The map label for this gene is rutA

Identifier: 15889766

GI number: 15889766

Start: 2468972

End: 2470063

Strand: Reverse

Name: rutA

Synonym: Atu2500

Alternate gene names: 15889766

Gene position: 2470063-2468972 (Counterclockwise)

Preceding gene: 159185244

Following gene: 15889765

Centisome position: 86.93

GC content: 57.42

Gene sequence:

>1092_bases
ATGGAAGTTGGTGTTTTTATCCCGATCGGGAACAATGGGTGGCTTCTTTCCGAAAACGCGCCGCAATACAAGCCGAGCTT
TGATCTGAACAAGGCGATCACGCTGAAGGCGGAGCAATATGGTTTCGACTTCGCGTTGTCGATGATCAAGCTGCGTGGCT
TTGGCGGCAAGACAGAGTTCTGGGACTATAATCTGGAATCCTTCACGCTGATGGCAGGCCTTGCCGCCGTCACCTCCAAG
ATCAAGCTGTTCGGCACCGCCGCCACGCTGGTCATGCCGCCCGCCATCGTCGCACGCATGGCGACCACGATCGATTCCAT
TTCCGGCGGCCGTTTCGGCATCAACCTGATTACCGGCTGGCAGCGCCCCGAGTACAGCCAGATGGGCCTTTGGCCGGGGG
ACGATTATTTCGGTGATCGCTATGAATATCTCGGCGAATATACCTCCGTGCTGAAAGAGCTGCTGACGGAAGGGCAGTCG
GACTTCAAGGGCAAGTTCTTCCAGATGGATGATTGCCGCATGAAGCCGGTGCCGCAGGGTGACGTGAAGCTCATCTGCGC
TGGTTCCTCCAATTCTGGCATGGCGTTTTCCGCCAAGTTTGCCGATTACAGCTTCTGCTTCGGCGTCGGCGTCAATACGC
CGAAGGCTTTTGCGCCCACCAATGAGCGACTTCTGGCAGCAACGGAAAAGTCCGGCCGTGAGGTTAAATCCGTCGTACTG
ACGATGGTGCTTGCCGAGGAAAAATCCGAGGATGCCTGGGCGAAGTGGGAGCACTACAAGGCGGGTGCGGATGAGGACGC
CATCAAGTGGCTCGGCCTGCAAAGCGCTGTCGACACCAAATCCGGCTCCGACACCAATGTAAGGCACATGTCTAACCCCG
TCTCGGCGGTCAACATCAACATGGGAACCTTGATCGGCTCCTATGAGGAAGTGGCGGCGATGCTGGATGAGATGAGCGAA
GTGCCGGGCACCGGCGGCGTGATGCTGACCTTTGACGACTTCCTCGAGGGCGTCGAAAAATTCGGCAAATATGTGCAGCC
GCTGATGAAGAGCCGCCAGCATGTGCTGGCCGAACTGGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
TTTATCCATTTGATAAAATTATTTTATTTGGTCTGGCACAGAACCTGCTACTCTTTTTGTCAAGTCAGGAGACACCTGAC
GTTAAAAAGGGAACTGCAAA

Downstream 100 bases:

>100_bases
GTGAAGCCGTCGTGGCGGGATATAAGGGACCTGAAAGCCGCTCGGAAAGCGTGACGCTTCCCGCGAGGCCGGAGCCGATT
ACCCTGAAACCCAGCGAGAC

Product: monooxygenase (pyrimidine utilization protein A)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MEVGVFIPIGNNGWLLSENAPQYKPSFDLNKAITLKAEQYGFDFALSMIKLRGFGGKTEFWDYNLESFTLMAGLAAVTSK
IKLFGTAATLVMPPAIVARMATTIDSISGGRFGINLITGWQRPEYSQMGLWPGDDYFGDRYEYLGEYTSVLKELLTEGQS
DFKGKFFQMDDCRMKPVPQGDVKLICAGSSNSGMAFSAKFADYSFCFGVGVNTPKAFAPTNERLLAATEKSGREVKSVVL
TMVLAEEKSEDAWAKWEHYKAGADEDAIKWLGLQSAVDTKSGSDTNVRHMSNPVSAVNINMGTLIGSYEEVAAMLDEMSE
VPGTGGVMLTFDDFLEGVEKFGKYVQPLMKSRQHVLAELEAAE

Sequences:

>Translated_363_residues
MEVGVFIPIGNNGWLLSENAPQYKPSFDLNKAITLKAEQYGFDFALSMIKLRGFGGKTEFWDYNLESFTLMAGLAAVTSK
IKLFGTAATLVMPPAIVARMATTIDSISGGRFGINLITGWQRPEYSQMGLWPGDDYFGDRYEYLGEYTSVLKELLTEGQS
DFKGKFFQMDDCRMKPVPQGDVKLICAGSSNSGMAFSAKFADYSFCFGVGVNTPKAFAPTNERLLAATEKSGREVKSVVL
TMVLAEEKSEDAWAKWEHYKAGADEDAIKWLGLQSAVDTKSGSDTNVRHMSNPVSAVNINMGTLIGSYEEVAAMLDEMSE
VPGTGGVMLTFDDFLEGVEKFGKYVQPLMKSRQHVLAELEAAE
>Mature_363_residues
MEVGVFIPIGNNGWLLSENAPQYKPSFDLNKAITLKAEQYGFDFALSMIKLRGFGGKTEFWDYNLESFTLMAGLAAVTSK
IKLFGTAATLVMPPAIVARMATTIDSISGGRFGINLITGWQRPEYSQMGLWPGDDYFGDRYEYLGEYTSVLKELLTEGQS
DFKGKFFQMDDCRMKPVPQGDVKLICAGSSNSGMAFSAKFADYSFCFGVGVNTPKAFAPTNERLLAATEKSGREVKSVVL
TMVLAEEKSEDAWAKWEHYKAGADEDAIKWLGLQSAVDTKSGSDTNVRHMSNPVSAVNINMGTLIGSYEEVAAMLDEMSE
VPGTGGVMLTFDDFLEGVEKFGKYVQPLMKSRQHVLAELEAAE

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=357, Percent_Identity=68.6274509803922, Blast_Score=539, Evalue=1e-155,
Organism=Escherichia coli, GI1787166, Length=303, Percent_Identity=24.7524752475248, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_AGRT5 (P58759)

Other databases:

- EMBL:   AE007869
- PIR:   AI2883
- PIR:   G97659
- RefSeq:   NP_355447.1
- ProteinModelPortal:   P58759
- STRING:   P58759
- GeneID:   1134538
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2500
- eggNOG:   COG2141
- HOGENOM:   HBG350183
- OMA:   ADYNFCF
- PhylomeDB:   P58759
- ProtClustDB:   CLSK879925
- BioCyc:   ATUM176299-1:ATU2500-MONOMER
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 39841; Mature: 39841

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: BINDING 115-115 BINDING 124-124 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVGVFIPIGNNGWLLSENAPQYKPSFDLNKAITLKAEQYGFDFALSMIKLRGFGGKTEF
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHCCCCCCCCE
WDYNLESFTLMAGLAAVTSKIKLFGTAATLVMPPAIVARMATTIDSISGGRFGINLITGW
EECCCHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECC
QRPEYSQMGLWPGDDYFGDRYEYLGEYTSVLKELLTEGQSDFKGKFFQMDDCRMKPVPQG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCC
DVKLICAGSSNSGMAFSAKFADYSFCFGVGVNTPKAFAPTNERLLAATEKSGREVKSVVL
CEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
TMVLAEEKSEDAWAKWEHYKAGADEDAIKWLGLQSAVDTKSGSDTNVRHMSNPVSAVNIN
HHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCHHHHCCCCEEEEEE
MGTLIGSYEEVAAMLDEMSEVPGTGGVMLTFDDFLEGVEKFGKYVQPLMKSRQHVLAELE
HHHHHCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAE
CCC
>Mature Secondary Structure
MEVGVFIPIGNNGWLLSENAPQYKPSFDLNKAITLKAEQYGFDFALSMIKLRGFGGKTEF
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEHHHCHHHHHHHHHHCCCCCCCCE
WDYNLESFTLMAGLAAVTSKIKLFGTAATLVMPPAIVARMATTIDSISGGRFGINLITGW
EECCCHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECC
QRPEYSQMGLWPGDDYFGDRYEYLGEYTSVLKELLTEGQSDFKGKFFQMDDCRMKPVPQG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCC
DVKLICAGSSNSGMAFSAKFADYSFCFGVGVNTPKAFAPTNERLLAATEKSGREVKSVVL
CEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
TMVLAEEKSEDAWAKWEHYKAGADEDAIKWLGLQSAVDTKSGSDTNVRHMSNPVSAVNIN
HHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCCCCHHHHCCCCEEEEEE
MGTLIGSYEEVAAMLDEMSEVPGTGGVMLTFDDFLEGVEKFGKYVQPLMKSRQHVLAELE
HHHHHCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194