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The map label for this gene is rutB [H]

Identifier: 15889765

GI number: 15889765

Start: 2468235

End: 2468975

Strand: Reverse

Name: rutB [H]

Synonym: Atu2499

Alternate gene names: 15889765

Gene position: 2468975-2468235 (Counterclockwise)

Preceding gene: 15889766

Following gene: 159185243

Centisome position: 86.89

GC content: 58.57

Gene sequence:

>741_bases
ATGAGTGAAGCCGTCGTGGCGGGATATAAGGGACCTGAAAGCCGCTCGGAAAGCGTGACGCTTCCCGCGAGGCCGGAGCC
GATTACCCTGAAACCCAGCGAGACCGCCGTTGTCGTGGTCGATATGCAGAACGCCTATTCGACCGAGGGCGGTTACGTCG
ACCTTGCCGGGTTCGATATTTCCGGGGCCAAGGGCACCATTGCCAACATCAAAAAGACGCTGGATGCGGCGCGGGCGGCG
GGCGTTCAGGTCATTTATTTCCAGAATGGCTGGGACAAGGATTATGTCGAGGCGGGCGGGCCGGGTTCGCCCAACTGGCA
CAAGTCCAACGCGCTGAAGACCATGCGCAAAAGGCCGGAACTGCAGGGCCAGCTGCTGGCCAAGGGCACATGGGATTATG
CCATTGTTGATGAGCTGCAACCGCAACCCGGCGATATTCTGGTGCCGAAGACGCGTTACAGCGGGTTCTTCAACACCAAT
ATGGACAGCGTTTTGCGTGCCCGCGGTATCCGCAATCTCGTCTTTGTCGGCATCGCCACCAATGTCTGTGTGGAAAGCTC
GCTGCGCGACGCGTTCCATCTCGAATATTTCGGGGTGATGCTGGAGGATGCCACCCATCATCTGGGTCCGGATTTTATCC
AGCAGGCGACGGTCTACAATGTCGAGAAGTTTTTCGGCTGGGTCGCCACCGTCAATGATTTCTGCGGCGTCATTTCGCAG
GCCGCGCCCGTTACCGATTGA

Upstream 100 bases:

>100_bases
CCTTTGACGACTTCCTCGAGGGCGTCGAAAAATTCGGCAAATATGTGCAGCCGCTGATGAAGAGCCGCCAGCATGTGCTG
GCCGAACTGGAGGCTGCGGA

Downstream 100 bases:

>100_bases
GCAGGAACCACAAAGAGGAGAACGAAATGCCGAAGAAGATCATCGTGCCAGTAGGCACCAGCAAACCCATCGCCCCTTTT
TCGCCGGGAACCCTTGCCGA

Product: isochorismatase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSEAVVAGYKGPESRSESVTLPARPEPITLKPSETAVVVVDMQNAYSTEGGYVDLAGFDISGAKGTIANIKKTLDAARAA
GVQVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKRPELQGQLLAKGTWDYAIVDELQPQPGDILVPKTRYSGFFNTN
MDSVLRARGIRNLVFVGIATNVCVESSLRDAFHLEYFGVMLEDATHHLGPDFIQQATVYNVEKFFGWVATVNDFCGVISQ
AAPVTD

Sequences:

>Translated_246_residues
MSEAVVAGYKGPESRSESVTLPARPEPITLKPSETAVVVVDMQNAYSTEGGYVDLAGFDISGAKGTIANIKKTLDAARAA
GVQVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKRPELQGQLLAKGTWDYAIVDELQPQPGDILVPKTRYSGFFNTN
MDSVLRARGIRNLVFVGIATNVCVESSLRDAFHLEYFGVMLEDATHHLGPDFIQQATVYNVEKFFGWVATVNDFCGVISQ
AAPVTD
>Mature_245_residues
SEAVVAGYKGPESRSESVTLPARPEPITLKPSETAVVVVDMQNAYSTEGGYVDLAGFDISGAKGTIANIKKTLDAARAAG
VQVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKRPELQGQLLAKGTWDYAIVDELQPQPGDILVPKTRYSGFFNTNM
DSVLRARGIRNLVFVGIATNVCVESSLRDAFHLEYFGVMLEDATHHLGPDFIQQATVYNVEKFFGWVATVNDFCGVISQA
APVTD

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=220, Percent_Identity=69.5454545454545, Blast_Score=340, Evalue=6e-95,
Organism=Escherichia coli, GI87081992, Length=178, Percent_Identity=29.7752808988764, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 26795; Mature: 26664

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAVVAGYKGPESRSESVTLPARPEPITLKPSETAVVVVDMQNAYSTEGGYVDLAGFDI
CCCCEEECCCCCCCCCCEEECCCCCCCEEECCCCCEEEEEECCCCCCCCCCEEEEECEEC
SGAKGTIANIKKTLDAARAAGVQVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCC
LQGQLLAKGTWDYAIVDELQPQPGDILVPKTRYSGFFNTNMDSVLRARGIRNLVFVGIAT
CCCEEEECCCCCEEEHHCCCCCCCCEEEECHHCCCEECCCHHHHHHHCCCCEEEEEEEHH
NVCVESSLRDAFHLEYFGVMLEDATHHLGPDFIQQATVYNVEKFFGWVATVNDFCGVISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AAPVTD
CCCCCC
>Mature Secondary Structure 
SEAVVAGYKGPESRSESVTLPARPEPITLKPSETAVVVVDMQNAYSTEGGYVDLAGFDI
CCCEEECCCCCCCCCCEEECCCCCCCEEECCCCCEEEEEECCCCCCCCCCEEEEECEEC
SGAKGTIANIKKTLDAARAAGVQVIYFQNGWDKDYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCC
LQGQLLAKGTWDYAIVDELQPQPGDILVPKTRYSGFFNTNMDSVLRARGIRNLVFVGIAT
CCCEEEECCCCCEEEHHCCCCCCCCEEEECHHCCCEECCCHHHHHHHCCCCEEEEEEEHH
NVCVESSLRDAFHLEYFGVMLEDATHHLGPDFIQQATVYNVEKFFGWVATVNDFCGVISQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AAPVTD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA