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The map label for this gene is cysS

Identifier: 15888416

GI number: 15888416

Start: 1064490

End: 1065875

Strand: Reverse

Name: cysS

Synonym: Atu1073

Alternate gene names: 15888416

Gene position: 1065875-1064490 (Counterclockwise)

Preceding gene: 15888417

Following gene: 159184604

Centisome position: 37.51

GC content: 61.4

Gene sequence:

>1386_bases
ATGTCCTTGCGCCTGAAACTCCACAACACCCTGACCCGCGAAAAGTCGGAATTTGCGCCGATCGACGCTGATAATGTGCG
CATGTATGTCTGCGGGCCAACGGTCTATGATTTCGCCCATATCGGCAATGCGCGCCCGGTCATCGTGTTCGATGTTCTCT
TCCGCCTGCTACGCCACGTCTATGGCGAAGACCACGTAACCTATGCCCGCAACATCACTGACCTCGACGACAAGATCAAC
GCGCGGGCACTGCGCGACTATCCGCATCTGCCGCTCAACGACGCCATCCATGCGGTGACGAAAAAGACCGCCGACCAGTT
CCATGATGACGTCGCCGCACTCGGCTGCCTTCAGCCGACTGTGGAGCCGCGCGCCACCGACTATATTGCCGAGATGATCT
ACCTCATCGAGAGGCTCATCGAACGCGGCCACGCCTATAAGGCGGGCGGCGAGGTTCTGTTCGACACCCGGTCGATGGCC
GACTACGGCCAGCTTTCCAAACGCCCGCTGGACGAACAGCAGGCCGGCGCGCGTGTTGCGGTGGAGGCCCACAAAAAGAA
CCCCGGCGATTTCGTGCTGTGGAAGCTCTCCTCCGAGAGCGAACCGGGCTGGGAGAGCCCATGGGGTCTCGGTCGCCCGG
GCTGGCACATCGAGTGCTCGGCCATGGCTGGCCGCTATCTCGGCGAAGTCTTCGATATTCACGGCGGCGGGCTCGACCTC
ATCTTCCCGCACCACGAAAACGAAATCGCGCAATCGCGCTGCGCCCATGGCACACATGTCATGGCCAATGTGTGGATGCA
TAACGGTTTCGTGCAGGTGGAAGGCCGCAAGATGTCGAAGTCCGAGGGCAACTTCGTGACGATCTACGAATTGCTGCACA
CGGATAAATTCGGCGGACGGCAATGGCCGGGCGAGGTTCTGCGCCTCGCCATGCTGATGACGCATTATCGCGAGCCGATC
GATTTTTCGGTCAAGCGTCTGGAAGAGGCCGAACGCCTGCTCTCCAAATGGCCGGCAGCCGAGACCTCCGACGCCGCGGC
GGATGAAACCGTGCTGGAGGCGCTCGCCGACGACCTCAACACGGTTGCGGCCGTGCAAGCACTGCATGCGCTGGCACATG
CGGCAAACACCGACCCATCCAGGCTGCCCGCCTTCGCCGCAAGCGCCGCCCTGCTGGGCGTCCTGCCGAAGAAGACGGAG
ATGGACGAGGCCATCGTTTCGGCCGTGGATGCACTGGTGGAATTGCGCCTTGAAATGCTGAAGGCGAAGAACTTTGCGGA
GGCCGACCGGTTGCGGGATGAGCTTTCCGAGAAGGGTATTCAGCTGAAGGATGGCAAGGACAAGGAGACTGGGGAACGGA
CGACGACGTGGGAGTTGAAGCGGTAG

Upstream 100 bases:

>100_bases
TTGCTGGTTCGCCACCGGCCAGATCGTTTTCGTCTTCCCGCAAGGTCCAACAGACAAACGGTCCGGTCAGGACAATTGAC
TGCCTTTAGGGAACACCCGC

Downstream 100 bases:

>100_bases
GCCTGCGTTGGTGGTTGGGGCTTACCCCCCTCTGCCCTGCCGGGCATCTCCCCCTCAAGGGGGGAGATCGGCAAGAGGCG
CTCCTACCACTGCATTCGCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSLRLKLHNTLTREKSEFAPIDADNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHVYGEDHVTYARNITDLDDKIN
ARALRDYPHLPLNDAIHAVTKKTADQFHDDVAALGCLQPTVEPRATDYIAEMIYLIERLIERGHAYKAGGEVLFDTRSMA
DYGQLSKRPLDEQQAGARVAVEAHKKNPGDFVLWKLSSESEPGWESPWGLGRPGWHIECSAMAGRYLGEVFDIHGGGLDL
IFPHHENEIAQSRCAHGTHVMANVWMHNGFVQVEGRKMSKSEGNFVTIYELLHTDKFGGRQWPGEVLRLAMLMTHYREPI
DFSVKRLEEAERLLSKWPAAETSDAAADETVLEALADDLNTVAAVQALHALAHAANTDPSRLPAFAASAALLGVLPKKTE
MDEAIVSAVDALVELRLEMLKAKNFAEADRLRDELSEKGIQLKDGKDKETGERTTTWELKR

Sequences:

>Translated_461_residues
MSLRLKLHNTLTREKSEFAPIDADNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHVYGEDHVTYARNITDLDDKIN
ARALRDYPHLPLNDAIHAVTKKTADQFHDDVAALGCLQPTVEPRATDYIAEMIYLIERLIERGHAYKAGGEVLFDTRSMA
DYGQLSKRPLDEQQAGARVAVEAHKKNPGDFVLWKLSSESEPGWESPWGLGRPGWHIECSAMAGRYLGEVFDIHGGGLDL
IFPHHENEIAQSRCAHGTHVMANVWMHNGFVQVEGRKMSKSEGNFVTIYELLHTDKFGGRQWPGEVLRLAMLMTHYREPI
DFSVKRLEEAERLLSKWPAAETSDAAADETVLEALADDLNTVAAVQALHALAHAANTDPSRLPAFAASAALLGVLPKKTE
MDEAIVSAVDALVELRLEMLKAKNFAEADRLRDELSEKGIQLKDGKDKETGERTTTWELKR
>Mature_460_residues
SLRLKLHNTLTREKSEFAPIDADNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHVYGEDHVTYARNITDLDDKINA
RALRDYPHLPLNDAIHAVTKKTADQFHDDVAALGCLQPTVEPRATDYIAEMIYLIERLIERGHAYKAGGEVLFDTRSMAD
YGQLSKRPLDEQQAGARVAVEAHKKNPGDFVLWKLSSESEPGWESPWGLGRPGWHIECSAMAGRYLGEVFDIHGGGLDLI
FPHHENEIAQSRCAHGTHVMANVWMHNGFVQVEGRKMSKSEGNFVTIYELLHTDKFGGRQWPGEVLRLAMLMTHYREPID
FSVKRLEEAERLLSKWPAAETSDAAADETVLEALADDLNTVAAVQALHALAHAANTDPSRLPAFAASAALLGVLPKKTEM
DEAIVSAVDALVELRLEMLKAKNFAEADRLRDELSEKGIQLKDGKDKETGERTTTWELKR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=527, Percent_Identity=33.3965844402277, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI303304987, Length=247, Percent_Identity=40.080971659919, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI10835051, Length=247, Percent_Identity=40.080971659919, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI62240992, Length=247, Percent_Identity=40.080971659919, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI21269877, Length=247, Percent_Identity=40.080971659919, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1786737, Length=483, Percent_Identity=44.9275362318841, Blast_Score=351, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI17509795, Length=251, Percent_Identity=36.6533864541833, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=36.6533864541833, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=312, Percent_Identity=37.1794871794872, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI19922218, Length=508, Percent_Identity=31.6929133858268, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI19922364, Length=349, Percent_Identity=33.5243553008596, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_AGRT5 (Q8UGG4)

Other databases:

- EMBL:   AE007869
- PIR:   A97491
- PIR:   AH2708
- RefSeq:   NP_354097.1
- ProteinModelPortal:   Q8UGG4
- SMR:   Q8UGG4
- STRING:   Q8UGG4
- GeneID:   1133111
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1073
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q8UGG4
- ProtClustDB:   PRK00260
- BioCyc:   ATUM176299-1:ATU1073-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51608; Mature: 51477

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLKLHNTLTREKSEFAPIDADNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHV
CCEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHH
YGEDHVTYARNITDLDDKINARALRDYPHLPLNDAIHAVTKKTADQFHDDVAALGCLQPT
HCCCCEEHHCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEPRATDYIAEMIYLIERLIERGHAYKAGGEVLFDTRSMADYGQLSKRPLDEQQAGARVA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCCCHHHCCCEEE
VEAHKKNPGDFVLWKLSSESEPGWESPWGLGRPGWHIECSAMAGRYLGEVFDIHGGGLDL
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEE
IFPHHENEIAQSRCAHGTHVMANVWMHNGFVQVEGRKMSKSEGNFVTIYELLHTDKFGGR
EECCCCCHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCC
QWPGEVLRLAMLMTHYREPIDFSVKRLEEAERLLSKWPAAETSDAAADETVLEALADDLN
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVAAVQALHALAHAANTDPSRLPAFAASAALLGVLPKKTEMDEAIVSAVDALVELRLEML
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAKNFAEADRLRDELSEKGIQLKDGKDKETGERTTTWELKR
HHCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SLRLKLHNTLTREKSEFAPIDADNVRMYVCGPTVYDFAHIGNARPVIVFDVLFRLLRHV
CEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHH
YGEDHVTYARNITDLDDKINARALRDYPHLPLNDAIHAVTKKTADQFHDDVAALGCLQPT
HCCCCEEHHCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEPRATDYIAEMIYLIERLIERGHAYKAGGEVLFDTRSMADYGQLSKRPLDEQQAGARVA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCCCHHHCCCEEE
VEAHKKNPGDFVLWKLSSESEPGWESPWGLGRPGWHIECSAMAGRYLGEVFDIHGGGLDL
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEE
IFPHHENEIAQSRCAHGTHVMANVWMHNGFVQVEGRKMSKSEGNFVTIYELLHTDKFGGR
EECCCCCHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCC
QWPGEVLRLAMLMTHYREPIDFSVKRLEEAERLLSKWPAAETSDAAADETVLEALADDLN
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVAAVQALHALAHAANTDPSRLPAFAASAALLGVLPKKTEMDEAIVSAVDALVELRLEML
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAKNFAEADRLRDELSEKGIQLKDGKDKETGERTTTWELKR
HHCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194