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The map label for this gene is fabI [H]

Identifier: 15888100

GI number: 15888100

Start: 754209

End: 755027

Strand: Reverse

Name: fabI [H]

Synonym: Atu0757

Alternate gene names: 15888100

Gene position: 755027-754209 (Counterclockwise)

Preceding gene: 159184453

Following gene: 15888099

Centisome position: 26.57

GC content: 58.73

Gene sequence:

>819_bases
ATGGCTCAGGCATCCGGCCTCATGGCTGGCAAACGCGGCCTCATCATGGGCGTCGCCAATAACCGTTCAATCGCTTGGGG
CATTGCAAAGGCCTGCGCGGATGCAGGTGCGGAACTCGCACTCACCTGGCAGGGTGACGCATTGAAGAAGCGCGTCGAGC
CTCTCGCGCAGGAACTCGGCGCTTTCATGGCCGGCCATTGCGACGTGACCGATCTCGAGACGATCGACTCCGTTTTTGCC
TCGCTGGAACAGCATTGGGGCAAGATCGACTTCGTCGTGCACGCCATTGCGTTCTCCGACAAAGACGAGCTGACCGGCCG
TTATCTCGATACCAGCCGCGACAACTTCAACCGCACCATGGATATTTCCGTGTTCTCGCTGGCCGCCGTTGCAAAGCGCG
CAGAGCCCATCATGAATGACGGCGGTTCGATCATCACGCTTACCTATTACGGCGCCGAAAAGGTCATGCCGAATTACAAC
GTGATGGGCGTGGCCAAGGCCGCTCTTGAAGCCAGCGTGCGTTATCTCGCCGTCGACCTCGGTAATCGCGGCATCCGCGT
CAACGCCGTTTCCGCCGGCCCGATCAAGACGCTTGCGGCTTCCGGCATCGGCGATTTCCGTTACATTCTGAAGTGGAACG
AATATAATGCGCCGCTGAAGCGCACCGTTACCATCGAGGAAGTCGGCAAGTCGGCGCTCTACCTGTTGTCCGACCTTTCG
ACGGCCGTGACCGGTGAAATCCACCATGTCGATTCCGGCTATCACACCATCGGCATGAAGGCAGTGGATGCGCCTGACAT
TTCGGTCGTTAAGGACTAA

Upstream 100 bases:

>100_bases
TTTGTTACGCTCCCTAACAGTTGATGATCTTTGGCAATCTTGAACAGGATTGCAGCCTATGCCATAGGCAGGACCGATCA
AAAGTATTAGGGAGCACAAA

Downstream 100 bases:

>100_bases
GCCTGCGACGTTTGAAAACGGGGTATCGCCTTGCTCGTCTATGTGATCCGACACGGACAAACGGACTGGAATGCGATACG
CCGGCTTCAGGGCCAGAAAG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MAQASGLMAGKRGLIMGVANNRSIAWGIAKACADAGAELALTWQGDALKKRVEPLAQELGAFMAGHCDVTDLETIDSVFA
SLEQHWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFNRTMDISVFSLAAVAKRAEPIMNDGGSIITLTYYGAEKVMPNYN
VMGVAKAALEASVRYLAVDLGNRGIRVNAVSAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVTIEEVGKSALYLLSDLS
TAVTGEIHHVDSGYHTIGMKAVDAPDISVVKD

Sequences:

>Translated_272_residues
MAQASGLMAGKRGLIMGVANNRSIAWGIAKACADAGAELALTWQGDALKKRVEPLAQELGAFMAGHCDVTDLETIDSVFA
SLEQHWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFNRTMDISVFSLAAVAKRAEPIMNDGGSIITLTYYGAEKVMPNYN
VMGVAKAALEASVRYLAVDLGNRGIRVNAVSAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVTIEEVGKSALYLLSDLS
TAVTGEIHHVDSGYHTIGMKAVDAPDISVVKD
>Mature_271_residues
AQASGLMAGKRGLIMGVANNRSIAWGIAKACADAGAELALTWQGDALKKRVEPLAQELGAFMAGHCDVTDLETIDSVFAS
LEQHWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFNRTMDISVFSLAAVAKRAEPIMNDGGSIITLTYYGAEKVMPNYNV
MGVAKAALEASVRYLAVDLGNRGIRVNAVSAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVTIEEVGKSALYLLSDLST
AVTGEIHHVDSGYHTIGMKAVDAPDISVVKD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=26.4591439688716, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI7705925, Length=251, Percent_Identity=23.5059760956175, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=27.3076923076923, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=50.1930501930502, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1789378, Length=262, Percent_Identity=29.0076335877863, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=29.277566539924, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=28.3400809716599, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=29.2490118577075, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=28.125, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=224, Percent_Identity=28.125, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29200; Mature: 29068

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQASGLMAGKRGLIMGVANNRSIAWGIAKACADAGAELALTWQGDALKKRVEPLAQELG
CCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
AFMAGHCDVTDLETIDSVFASLEQHWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFNRTM
HHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHCCCEEECCCCCCCCEEE
DISVFSLAAVAKRAEPIMNDGGSIITLTYYGAEKVMPNYNVMGVAKAALEASVRYLAVDL
EHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHCEEEEEEEE
GNRGIRVNAVSAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVTIEEVGKSALYLLSDLS
CCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCHHEEEEHHHHCCHHHHHHHHHH
TAVTGEIHHVDSGYHTIGMKAVDAPDISVVKD
HHHCCEEEEECCCCEEECEEEECCCCCEEECC
>Mature Secondary Structure 
AQASGLMAGKRGLIMGVANNRSIAWGIAKACADAGAELALTWQGDALKKRVEPLAQELG
CCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
AFMAGHCDVTDLETIDSVFASLEQHWGKIDFVVHAIAFSDKDELTGRYLDTSRDNFNRTM
HHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHCCCEEECCCCCCCCEEE
DISVFSLAAVAKRAEPIMNDGGSIITLTYYGAEKVMPNYNVMGVAKAALEASVRYLAVDL
EHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHCEEEEEEEE
GNRGIRVNAVSAGPIKTLAASGIGDFRYILKWNEYNAPLKRTVTIEEVGKSALYLLSDLS
CCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCHHEEEEHHHHCCHHHHHHHHHH
TAVTGEIHHVDSGYHTIGMKAVDAPDISVVKD
HHHCCEEEEECCCCEEECEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]