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The map label for this gene is potI [H]

Identifier: 15887957

GI number: 15887957

Start: 599651

End: 600463

Strand: Direct

Name: potI [H]

Synonym: Atu0612

Alternate gene names: 15887957

Gene position: 599651-600463 (Clockwise)

Preceding gene: 159184388

Following gene: 15887961

Centisome position: 21.1

GC content: 59.41

Gene sequence:

>813_bases
ATGAAGCGCGGAAAATTCGACATCACGGTCCTGACGCTCGGTTTTGCCTTTCTCTATATCCCGATCATCATCCTGATCAT
TTATTCCTTCAATGCCTCCAAGCTCGTCACCGTCTGGGGCGGCTTTTCGCTGCAATGGTATAAGTCCATGTGGTCGAACC
AGGGGCTGATGGATGCGGCCTGGGTGACGCTGCGTGTCGGTATCCTCAGCGCCACCATCGGCACCATCCTCGGAACGCTG
GCGGCTCTGTCTCTCACCCGTTTTGCCCGTTTCCCGGGCCGGGTGCTGTTTTCCGGCATGATCTATGCGCCGCTCGTCAT
GCCCGAGGTCATCACCGGCCTGTCGTTGCTGCTTTTGTTCGTGGCCGTTGGGGTGGATCGTGGTTTCTGGACTGTCGTCA
TCGCCCATACGACCTTCACCATGTGTTATGTGGCAATCGTCGTACAGTCGCGCCTCCTGACCTTCGACCGCAGTCTTGAG
GAGGCGGCGCTCGATCTTGGTTGCCCGCCGGTCAAGACCTTCTTCCGCATCACCCTGCCGCTGATTTTCCCGGCCGTGAT
TGCCGGCTGGATGCTCGCTTTCACCCTGTCGCTCGACGATCTCGTGATCGCCAGCTTTGCCACCGGCCCGGGTGCGACGA
CGCTGCCGATCAAGATTTATTCGCAGGTTCGTTTGGGCGTGACGCCGGAAATCAACGCCATCTGCACCATCCTGATCGGT
CTCGTGACCATCGGCGTCATTGCCGCTTCCGTCGCCTCCAAGCGCACCGAATTGCAGCGGATGCGCGACGAACATGCGGC
TGCGCGAAACTGA

Upstream 100 bases:

>100_bases
GACTGGCCCTTGGCATCCGCTGTCGCCGTGCTGTTGCTGATCCTGCTGGTGGTGCCCATCGCCATCTTCCAGAACCAGCA
GAAGAAGGTGGGGTGAGGCC

Downstream 100 bases:

>100_bases
ATAAACGCCGTCCCGATTTCTTCGCGTTACTATTGAAATCGCGATAAAATCAGGAAGCTGTAATTTAGTTTTGGCTCTTT
ACGGTCTCAGAATAGGAATT

Product: ABC transporter, membrane spanning protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKRGKFDITVLTLGFAFLYIPIIILIIYSFNASKLVTVWGGFSLQWYKSMWSNQGLMDAAWVTLRVGILSATIGTILGTL
AALSLTRFARFPGRVLFSGMIYAPLVMPEVITGLSLLLLFVAVGVDRGFWTVVIAHTTFTMCYVAIVVQSRLLTFDRSLE
EAALDLGCPPVKTFFRITLPLIFPAVIAGWMLAFTLSLDDLVIASFATGPGATTLPIKIYSQVRLGVTPEINAICTILIG
LVTIGVIAASVASKRTELQRMRDEHAAARN

Sequences:

>Translated_270_residues
MKRGKFDITVLTLGFAFLYIPIIILIIYSFNASKLVTVWGGFSLQWYKSMWSNQGLMDAAWVTLRVGILSATIGTILGTL
AALSLTRFARFPGRVLFSGMIYAPLVMPEVITGLSLLLLFVAVGVDRGFWTVVIAHTTFTMCYVAIVVQSRLLTFDRSLE
EAALDLGCPPVKTFFRITLPLIFPAVIAGWMLAFTLSLDDLVIASFATGPGATTLPIKIYSQVRLGVTPEINAICTILIG
LVTIGVIAASVASKRTELQRMRDEHAAARN
>Mature_270_residues
MKRGKFDITVLTLGFAFLYIPIIILIIYSFNASKLVTVWGGFSLQWYKSMWSNQGLMDAAWVTLRVGILSATIGTILGTL
AALSLTRFARFPGRVLFSGMIYAPLVMPEVITGLSLLLLFVAVGVDRGFWTVVIAHTTFTMCYVAIVVQSRLLTFDRSLE
EAALDLGCPPVKTFFRITLPLIFPAVIAGWMLAFTLSLDDLVIASFATGPGATTLPIKIYSQVRLGVTPEINAICTILIG
LVTIGVIAASVASKRTELQRMRDEHAAARN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=274, Percent_Identity=54.3795620437956, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1787368, Length=232, Percent_Identity=43.1034482758621, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787714, Length=239, Percent_Identity=33.0543933054393, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788764, Length=176, Percent_Identity=30.1136363636364, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29470; Mature: 29470

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGKFDITVLTLGFAFLYIPIIILIIYSFNASKLVTVWGGFSLQWYKSMWSNQGLMDAA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
WVTLRVGILSATIGTILGTLAALSLTRFARFPGRVLFSGMIYAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVGVDRGFWTVVIAHTTFTMCYVAIVVQSRLLTFDRSLEEAALDLGCPPVKTFFRITLP
HHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIFPAVIAGWMLAFTLSLDDLVIASFATGPGATTLPIKIYSQVRLGVTPEINAICTILIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEEHHHCCCCCCHHHHHHHHHH
LVTIGVIAASVASKRTELQRMRDEHAAARN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRGKFDITVLTLGFAFLYIPIIILIIYSFNASKLVTVWGGFSLQWYKSMWSNQGLMDAA
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
WVTLRVGILSATIGTILGTLAALSLTRFARFPGRVLFSGMIYAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAVGVDRGFWTVVIAHTTFTMCYVAIVVQSRLLTFDRSLEEAALDLGCPPVKTFFRITLP
HHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIFPAVIAGWMLAFTLSLDDLVIASFATGPGATTLPIKIYSQVRLGVTPEINAICTILIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEEEHHHCCCCCCHHHHHHHHHH
LVTIGVIAASVASKRTELQRMRDEHAAARN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]