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The map label for this gene is potB [H]

Identifier: 159184388

GI number: 159184388

Start: 598738

End: 599646

Strand: Direct

Name: potB [H]

Synonym: Atu0611

Alternate gene names: 159184388

Gene position: 598738-599646 (Clockwise)

Preceding gene: 15887955

Following gene: 15887957

Centisome position: 21.07

GC content: 58.53

Gene sequence:

>909_bases
ATGGCGATCACCACTCCCGAGAACCGGCAAAGCCCCTGGCGCTGGCTGGTCGTTGCGGTTCCCTATTTCTGGCTGCTGCT
GTTTTTCGCCGCACCCTTTCTCATCATCGTCAAGATTTCGCTGTCGGATACGGCGATCTCCATGCCGCCCTATACGCCTG
TCTTCGAAGGGTTTTCGAAGCTCGGCGAATTTTTCTCGCAGCTGGATTTCGAGAATTATCTCTTCCTGACGGAAGACCCG
CTCTACATTGAAGCCTATCTGTCGTCGCTGCGCATCGCCTTCATCTCTACCTTCCTACTGCTGCTGATCGGTTATCCCAT
GGCGCTGGCCATGGCGCGCGCACCGTCTTCCGTGCGCCCGACGCTGGTGATGCTGGTGATCCTGCCGTTCTGGACCTCGT
TCCTGATCCGCGTTTACGCCTGGATCGGCATTCTGAAGCCGGAAGGCCTGTTGACGGTGCTGTTTCAGTGGCTTGGGTTT
CTCGGACCAGACCAGCAGGTCAATATCTTCCGCACCGAGACGGCGATCTTCATCGGCATCGTTTATTCCTATCTGCCCTT
CATGGTCCTGCCGCTTTATTCGGCGCTGGAAAAACTCGACAATACGCTGCTTGAGGCGGCCGCCGATCTCGGTTGCCCGC
CCTGGAAGGCCTTCTGGAAAATCACTTTCCCGCTCTCCCTGCCGGGCGTGGTCGCCGGGGCGATGATCTGCTTCATCCCG
ATAACAGGCGAGTTCGTCATTCCCGATCTGCTGGGCGGCGCCCAGACGCTGATGATCGGCAAGACGCTGTGGACGGAATT
CTTCGGCAATCGCGACTGGCCCTTGGCATCCGCTGTCGCCGTGCTGTTGCTGATCCTGCTGGTGGTGCCCATCGCCATCT
TCCAGAACCAGCAGAAGAAGGTGGGGTGA

Upstream 100 bases:

>100_bases
TCTCGCGTCTCGTCGATCGCCCCATCACTTTCGGCGATATGGTGTGGCTGAACTGGAAACCGGATTCAGGGCTTGTCCTG
ACACGCTGAGGGAGGCTTCG

Downstream 100 bases:

>100_bases
GGCCATGAAGCGCGGAAAATTCGACATCACGGTCCTGACGCTCGGTTTTGCCTTTCTCTATATCCCGATCATCATCCTGA
TCATTTATTCCTTCAATGCC

Product: ABC transporter, membrane spanning protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MAITTPENRQSPWRWLVVAVPYFWLLLFFAAPFLIIVKISLSDTAISMPPYTPVFEGFSKLGEFFSQLDFENYLFLTEDP
LYIEAYLSSLRIAFISTFLLLLIGYPMALAMARAPSSVRPTLVMLVILPFWTSFLIRVYAWIGILKPEGLLTVLFQWLGF
LGPDQQVNIFRTETAIFIGIVYSYLPFMVLPLYSALEKLDNTLLEAAADLGCPPWKAFWKITFPLSLPGVVAGAMICFIP
ITGEFVIPDLLGGAQTLMIGKTLWTEFFGNRDWPLASAVAVLLLILLVVPIAIFQNQQKKVG

Sequences:

>Translated_302_residues
MAITTPENRQSPWRWLVVAVPYFWLLLFFAAPFLIIVKISLSDTAISMPPYTPVFEGFSKLGEFFSQLDFENYLFLTEDP
LYIEAYLSSLRIAFISTFLLLLIGYPMALAMARAPSSVRPTLVMLVILPFWTSFLIRVYAWIGILKPEGLLTVLFQWLGF
LGPDQQVNIFRTETAIFIGIVYSYLPFMVLPLYSALEKLDNTLLEAAADLGCPPWKAFWKITFPLSLPGVVAGAMICFIP
ITGEFVIPDLLGGAQTLMIGKTLWTEFFGNRDWPLASAVAVLLLILLVVPIAIFQNQQKKVG
>Mature_301_residues
AITTPENRQSPWRWLVVAVPYFWLLLFFAAPFLIIVKISLSDTAISMPPYTPVFEGFSKLGEFFSQLDFENYLFLTEDPL
YIEAYLSSLRIAFISTFLLLLIGYPMALAMARAPSSVRPTLVMLVILPFWTSFLIRVYAWIGILKPEGLLTVLFQWLGFL
GPDQQVNIFRTETAIFIGIVYSYLPFMVLPLYSALEKLDNTLLEAAADLGCPPWKAFWKITFPLSLPGVVAGAMICFIPI
TGEFVIPDLLGGAQTLMIGKTLWTEFFGNRDWPLASAVAVLLLILLVVPIAIFQNQQKKVG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=290, Percent_Identity=55.5172413793103, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=44.7004608294931, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787713, Length=228, Percent_Identity=31.140350877193, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33951; Mature: 33820

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITTPENRQSPWRWLVVAVPYFWLLLFFAAPFLIIVKISLSDTAISMPPYTPVFEGFSK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCHHHHHHHH
LGEFFSQLDFENYLFLTEDPLYIEAYLSSLRIAFISTFLLLLIGYPMALAMARAPSSVRP
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TLVMLVILPFWTSFLIRVYAWIGILKPEGLLTVLFQWLGFLGPDQQVNIFRTETAIFIGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHH
VYSYLPFMVLPLYSALEKLDNTLLEAAADLGCPPWKAFWKITFPLSLPGVVAGAMICFIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHEEE
ITGEFVIPDLLGGAQTLMIGKTLWTEFFGNRDWPLASAVAVLLLILLVVPIAIFQNQQKK
CCCCEECHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHC
VG
CC
>Mature Secondary Structure 
AITTPENRQSPWRWLVVAVPYFWLLLFFAAPFLIIVKISLSDTAISMPPYTPVFEGFSK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCHHHHHHHH
LGEFFSQLDFENYLFLTEDPLYIEAYLSSLRIAFISTFLLLLIGYPMALAMARAPSSVRP
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TLVMLVILPFWTSFLIRVYAWIGILKPEGLLTVLFQWLGFLGPDQQVNIFRTETAIFIGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHH
VYSYLPFMVLPLYSALEKLDNTLLEAAADLGCPPWKAFWKITFPLSLPGVVAGAMICFIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHEEE
ITGEFVIPDLLGGAQTLMIGKTLWTEFFGNRDWPLASAVAVLLLILLVVPIAIFQNQQKK
CCCCEECHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHC
VG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]