Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 15887721

GI number: 15887721

Start: 363738

End: 364934

Strand: Reverse

Name: dapE

Synonym: Atu0370

Alternate gene names: 15887721

Gene position: 364934-363738 (Counterclockwise)

Preceding gene: 159184277

Following gene: 15887720

Centisome position: 12.84

GC content: 62.49

Gene sequence:

>1197_bases
ATGACCACGACCGATCCCGTTGCCAATCTCGCCGCCCTCATCCGTTGCCCGTCGGTAACGCCTGCGGAAGGCGGCGCGCT
TTCCCTGCTCGACACCCTGCTTTCACCGCTTGGTTTTGCGGTGGAGCGCGTGATGGCGACAGAGGACGGCACGCCTGATG
TGGAGAACCTCTACGCCCGGCTCGGCACCGAAGGTCCGCATCTGATGTTTGCGGGCCATACGGATGTCGTTCCCGTCGGC
GACGAGGCCGCATGGAGCCATCCGCCCTTTTCGGCAGACATCGCCGGTGGCGAAATGTACGGTCGCGGCGCGGTGGACAT
GAAGGGCGGCATTGCCTGTTTCGTCGCCGCCATCGCCCGCCATATCGGAAAACATGGCAAACCGCAGGGCTCGGTTTCCT
TCCTGATCACCGGAGACGAGGAAGGCCCGTCGATCAACGGCACCTCCAAGCTTCTGGAATGGGCTGCCGCAAAGGGAGAG
ACGTGGGACGCCTGTGTCGTCGGCGAGCCTACCAATCCCGACCAGCTGGGCGACATGATCAAGATCGGCCGCCGGGGATC
GCTCTCGGGCCGGATCACCGTCCAAGGCGTTCAAGGCCACGCCGCCTATCCACATCTCGCCGACAACCCCATTCGTGGTC
TGCTGCAGCTCACCCATGCGCTGATGCATCCAGCCTTCGACCACGGCACGGATGATTTCCAGCCATCCAATCTGGAAGTA
ACGACCGTCGACACCGGCAATGCCGCGACCAACGTCATTCCGGCGCGGGCGACGGCAGCCTTCAACATTCGTTTCAACGA
TAGCTGGACGGCAGAGAGCCTGCGGGCGGAGATCATCCGCCGCCTCGATGCAGCGGCAAATGAGGGCGAGCTTCGCCCCG
ACCGGGCTCCGGTCAAATACGAGATCGTCTGGGCGGACCGTCCGAGCCATGTCTTCTTGACCCGCAACAATGCGCTCATC
TCGTCGCTGTCAGGCGCGATTGAAACCGTAACCGGCAAGGAGCCAAAGCTTTCGACCACCGGCGGCACCTCGGATGCACG
CTTCATCAAGGATTATTGCCCTGTCGTGGAATTCGGTCTCGTCGGGCAGACCATGCACATGGTCGATGAACGCGTGGCAG
TTGCCGATCTCGAGACGCTGACGCGCATTTACGAGACCTTCATCGAACGCTGGTTCGCCCATGCCGACGGCAAGTGA

Upstream 100 bases:

>100_bases
CGAGCCTTTATTGCGCCGTCATCGTCAAGCGCGTCGATGAAAAGACCCGCTCCAAGACCGGCATCAACGAGCTTCTGCGC
GACTGATAAAAGCTGATACC

Downstream 100 bases:

>100_bases
AGTAAGGCTCTATCTGACAGGGCTGTGGCTGCTGCTGCTCGGCGACCATAATGGCGCGCGGTATCTGGATCTTTCCGAGC
GCGGAATGTGGCGATCCTTC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTTTDPVANLAALIRCPSVTPAEGGALSLLDTLLSPLGFAVERVMATEDGTPDVENLYARLGTEGPHLMFAGHTDVVPVG
DEAAWSHPPFSADIAGGEMYGRGAVDMKGGIACFVAAIARHIGKHGKPQGSVSFLITGDEEGPSINGTSKLLEWAAAKGE
TWDACVVGEPTNPDQLGDMIKIGRRGSLSGRITVQGVQGHAAYPHLADNPIRGLLQLTHALMHPAFDHGTDDFQPSNLEV
TTVDTGNAATNVIPARATAAFNIRFNDSWTAESLRAEIIRRLDAAANEGELRPDRAPVKYEIVWADRPSHVFLTRNNALI
SSLSGAIETVTGKEPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVADLETLTRIYETFIERWFAHADGK

Sequences:

>Translated_398_residues
MTTTDPVANLAALIRCPSVTPAEGGALSLLDTLLSPLGFAVERVMATEDGTPDVENLYARLGTEGPHLMFAGHTDVVPVG
DEAAWSHPPFSADIAGGEMYGRGAVDMKGGIACFVAAIARHIGKHGKPQGSVSFLITGDEEGPSINGTSKLLEWAAAKGE
TWDACVVGEPTNPDQLGDMIKIGRRGSLSGRITVQGVQGHAAYPHLADNPIRGLLQLTHALMHPAFDHGTDDFQPSNLEV
TTVDTGNAATNVIPARATAAFNIRFNDSWTAESLRAEIIRRLDAAANEGELRPDRAPVKYEIVWADRPSHVFLTRNNALI
SSLSGAIETVTGKEPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVADLETLTRIYETFIERWFAHADGK
>Mature_397_residues
TTTDPVANLAALIRCPSVTPAEGGALSLLDTLLSPLGFAVERVMATEDGTPDVENLYARLGTEGPHLMFAGHTDVVPVGD
EAAWSHPPFSADIAGGEMYGRGAVDMKGGIACFVAAIARHIGKHGKPQGSVSFLITGDEEGPSINGTSKLLEWAAAKGET
WDACVVGEPTNPDQLGDMIKIGRRGSLSGRITVQGVQGHAAYPHLADNPIRGLLQLTHALMHPAFDHGTDDFQPSNLEVT
TVDTGNAATNVIPARATAAFNIRFNDSWTAESLRAEIIRRLDAAANEGELRPDRAPVKYEIVWADRPSHVFLTRNNALIS
SLSGAIETVTGKEPKLSTTGGTSDARFIKDYCPVVEFGLVGQTMHMVDERVAVADLETLTRIYETFIERWFAHADGK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=392, Percent_Identity=39.2857142857143, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1790395, Length=225, Percent_Identity=32, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_AGRT5 (A9CKC4)

Other databases:

- EMBL:   AE007869
- PIR:   AB2622
- PIR:   B97404
- RefSeq:   NP_353402.1
- ProteinModelPortal:   A9CKC4
- SMR:   A9CKC4
- STRING:   A9CKC4
- MEROPS:   M20.010
- GeneID:   1132408
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0370
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- PhylomeDB:   A9CKC4
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42547; Mature: 42416

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 75-75 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTDPVANLAALIRCPSVTPAEGGALSLLDTLLSPLGFAVERVMATEDGTPDVENLYAR
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LGTEGPHLMFAGHTDVVPVGDEAAWSHPPFSADIAGGEMYGRGAVDMKGGIACFVAAIAR
HCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
HIGKHGKPQGSVSFLITGDEEGPSINGTSKLLEWAAAKGETWDACVVGEPTNPDQLGDMI
HHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
KIGRRGSLSGRITVQGVQGHAAYPHLADNPIRGLLQLTHALMHPAFDHGTDDFQPSNLEV
HHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TTVDTGNAATNVIPARATAAFNIRFNDSWTAESLRAEIIRRLDAAANEGELRPDRAPVKY
EEEECCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
EIVWADRPSHVFLTRNNALISSLSGAIETVTGKEPKLSTTGGTSDARFIKDYCPVVEFGL
EEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
VGQTMHMVDERVAVADLETLTRIYETFIERWFAHADGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTDPVANLAALIRCPSVTPAEGGALSLLDTLLSPLGFAVERVMATEDGTPDVENLYAR
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LGTEGPHLMFAGHTDVVPVGDEAAWSHPPFSADIAGGEMYGRGAVDMKGGIACFVAAIAR
HCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHH
HIGKHGKPQGSVSFLITGDEEGPSINGTSKLLEWAAAKGETWDACVVGEPTNPDQLGDMI
HHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
KIGRRGSLSGRITVQGVQGHAAYPHLADNPIRGLLQLTHALMHPAFDHGTDDFQPSNLEV
HHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
TTVDTGNAATNVIPARATAAFNIRFNDSWTAESLRAEIIRRLDAAANEGELRPDRAPVKY
EEEECCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
EIVWADRPSHVFLTRNNALISSLSGAIETVTGKEPKLSTTGGTSDARFIKDYCPVVEFGL
EEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
VGQTMHMVDERVAVADLETLTRIYETFIERWFAHADGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743194; 11743193