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The map label for this gene is grpE

Identifier: 15887680

GI number: 15887680

Start: 323105

End: 323740

Strand: Direct

Name: grpE

Synonym: Atu0329

Alternate gene names: 15887680

Gene position: 323105-323740 (Clockwise)

Preceding gene: 15887679

Following gene: 15887687

Centisome position: 11.37

GC content: 60.06

Gene sequence:

>636_bases
ATGACCGACGATACAAAAAAGCCCGGACCTGACGCGGACGTCGCGGAAGAGTTTGTCGATCCTGCCCAGGCAGGTGAAGA
ACAGGCCGAAACGGCAGAGCCCGATCCGGTGGAACTGCTCAAGGCTGAGAACGCCGATCTGCGCGATAAATTCCTCCGCC
TTGCAGCCGAGATGGACAATCTTCGCCGCCGCACCGAGCGTGACGTCAAGGATGCCAAGGCCTATTCGCTGGCCGGTTTT
GCCCGCGACATGCTTGCCGTTTCCGATAATCTTCGCCGCGCGCTGGAAGCCATTCCGGACGAACTCAAGACCAATGGCGA
AGCCGGTCTCAACGGCCTGATCGAAGGCGTGGAAATGACCGAGCGCTCGATGCTGTCGACGCTGGAGCGCCACGGTGTGA
AGAAGATCGATGCCGAAGGCCAGAAATTCGACCCGAATTTCCATCAGGCCATGTTCGAAGTTCCGAACACGGCGGTTCCC
AACAATACCGTACTTCAGGTCATTCAGGCCGGTTTCACCATCGGTGACCGCGTTCTGCGCCCCGCGATGGTGGGTGTCGC
CAAGGGCGGCCCGAAGGCTGAACCCTCCGCATCGGCCGAGCCGGGCACGTCGTCTCTTAATGAAAAGGACGCGTGA

Upstream 100 bases:

>100_bases
ATCAGAGAATGCGGCAAGCTTTTTGCCGCAAGCCTTGATTTTTGCCCGTCAAAGCTCGATATCGGGCCCAACCCAATAAC
AGTCTAATCTGGAGAACGTC

Downstream 100 bases:

>100_bases
GGCATTTATCCTTCCGTCATAAAAAACGCGCCTTCAGGGCGCGTTTTTTATTGTCGTTAAACTGACAGGATAGGCCCGCG
ATCAGGCCGCCGTCGCCTGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTDDTKKPGPDADVAEEFVDPAQAGEEQAETAEPDPVELLKAENADLRDKFLRLAAEMDNLRRRTERDVKDAKAYSLAGF
ARDMLAVSDNLRRALEAIPDELKTNGEAGLNGLIEGVEMTERSMLSTLERHGVKKIDAEGQKFDPNFHQAMFEVPNTAVP
NNTVLQVIQAGFTIGDRVLRPAMVGVAKGGPKAEPSASAEPGTSSLNEKDA

Sequences:

>Translated_211_residues
MTDDTKKPGPDADVAEEFVDPAQAGEEQAETAEPDPVELLKAENADLRDKFLRLAAEMDNLRRRTERDVKDAKAYSLAGF
ARDMLAVSDNLRRALEAIPDELKTNGEAGLNGLIEGVEMTERSMLSTLERHGVKKIDAEGQKFDPNFHQAMFEVPNTAVP
NNTVLQVIQAGFTIGDRVLRPAMVGVAKGGPKAEPSASAEPGTSSLNEKDA
>Mature_210_residues
TDDTKKPGPDADVAEEFVDPAQAGEEQAETAEPDPVELLKAENADLRDKFLRLAAEMDNLRRRTERDVKDAKAYSLAGFA
RDMLAVSDNLRRALEAIPDELKTNGEAGLNGLIEGVEMTERSMLSTLERHGVKKIDAEGQKFDPNFHQAMFEVPNTAVPN
NTVLQVIQAGFTIGDRVLRPAMVGVAKGGPKAEPSASAEPGTSSLNEKDA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=140, Percent_Identity=37.1428571428571, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1788967, Length=142, Percent_Identity=40.1408450704225, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=181, Percent_Identity=37.0165745856354, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324806, Length=148, Percent_Identity=42.5675675675676, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24653432, Length=174, Percent_Identity=38.5057471264368, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_AGRT5 (P63187)

Other databases:

- EMBL:   AE007869
- PIR:   A97399
- PIR:   AI2616
- RefSeq:   NP_353361.1
- ProteinModelPortal:   P63187
- SMR:   P63187
- STRING:   P63187
- GeneID:   1132367
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0329
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- PhylomeDB:   P63187
- ProtClustDB:   PRK14141
- BioCyc:   ATUM176299-1:ATU0329-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22831; Mature: 22700

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDTKKPGPDADVAEEFVDPAQAGEEQAETAEPDPVELLKAENADLRDKFLRLAAEMDN
CCCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LRRRTERDVKDAKAYSLAGFARDMLAVSDNLRRALEAIPDELKTNGEAGLNGLIEGVEMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERSMLSTLERHGVKKIDAEGQKFDPNFHQAMFEVPNTAVPNNTVLQVIQAGFTIGDRVLR
HHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHH
PAMVGVAKGGPKAEPSASAEPGTSSLNEKDA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDDTKKPGPDADVAEEFVDPAQAGEEQAETAEPDPVELLKAENADLRDKFLRLAAEMDN
CCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LRRRTERDVKDAKAYSLAGFARDMLAVSDNLRRALEAIPDELKTNGEAGLNGLIEGVEMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERSMLSTLERHGVKKIDAEGQKFDPNFHQAMFEVPNTAVPNNTVLQVIQAGFTIGDRVLR
HHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHH
PAMVGVAKGGPKAEPSASAEPGTSSLNEKDA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194