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The map label for this gene is nusA [H]

Identifier: 15887443

GI number: 15887443

Start: 92292

End: 93908

Strand: Reverse

Name: nusA [H]

Synonym: Atu0089

Alternate gene names: 15887443

Gene position: 93908-92292 (Counterclockwise)

Preceding gene: 15887444

Following gene: 15887442

Centisome position: 3.3

GC content: 60.17

Gene sequence:

>1617_bases
ATGGCAGTGAGTGCTAACCGGCTTGAGCTTCTGCAGATCGCCGATGCTGTGGCGCGCGAAAAGGTCATCGACCGCGAAAT
CGTGCTTGCCGCAATGGCCGACGCTATCCAGAAAGCCGCCCGCTCGCGCTATGGTTCGGAAACCAACATCCGCGCCGACA
TCAACTCCAAGACCGGCGAAATCCGCCTGCAGCGTCTTCTGGAAGTGGTGGAAGCAGCCGAAGACTATTCGACCCAGATT
CCGCTCGAACTTGCCCGCGACCGCAACCCCGACGCCAAGCTCGGCGACTTCATCGCCGATCCGCTGCCGCCGATGGATTT
CGGCCGTATCGCTGCCCAGTCCGCCAAGCAGGTCATCGTGCAGAAGGTACGTGAAGCCGAGCGCGATCGTCAGTATGACG
AGTTCAAGGACCGCATCGGCGAAATCGTCAACGGCACCGTGAAGCGCGTCGAATATGGCAATGTCATCGTCGATCTCGGC
CGTGGCGAAGGCATCATCCGTCGCGACGAGATGATCCCGCGCGAAAACATGCGTTATGGCGACCGCGTTCGCGCCTATGT
CTACGACGTTCGCCGCGAACAGCGTGGCCCGCAGATTTTCCTGTCGCGTACCCATCCGCAGTTCATGGTGAAGCTGTTCA
CCATGGAAGTCCCGGAAATCTACGACGGCATCATCCAGATCAAGTCGGTTGCCCGTGACCCCGGTTCGCGCGCCAAAATC
GCCGTCATCTCGAACGATAGCTCGATCGATCCGGTCGGCGCCTGCGTCGGTATGCGCGGTTCGCGCGTTCAGGCCGTTGT
CGGCGAACTCCAGGGCGAGAAGATCGACATCATTCCGTGGAGCCAGGAACCGGCTTCCTTCATCGTCAACGCCCTGCAGC
CGGCGGAAGTCGCCAAAGTCGTTCTGGATGAAGAATCCGAGCGTATCGAAGTGGTTGTTCCCGACGAGCAGCTGTCGCTC
GCCATCGGCCGCCGCGGTCAGAACGTGCGTCTGGCATCGCAGCTGACCGGCTGGGATATCGACATCATGACCGAGCAGGA
AGAATCCGAGCGTCGCCAGAAGGAATTCAACGAGCGCACGGCACTCTTCATGGAAGCGCTGGATGTCGACGAAATGGTCG
GCCAGGTTCTGGCTTCGGAAGGGTTCGCGCAGGTTGAAGAACTGGCCTATGTCGATCTCGACGAAATTACCTCGATCGAT
GGCTTCGACGAAGACACTGCTGATGAAATCCAGACCCGCGCCCGCGAATATCTGGAACGGCTGGAAGCCGAAATGGACGC
CAAGCGCAAGGAACTCGGCGTTACCGACGAGCTGCGCCAGATCGATGGCCTGACCTCGCAGATGATGGTCGCGCTCGGTG
AAGACGGCATCAAGACCATCGAGGACTTTGCCGGCTGCGCAGCGGACGATCTGGTCGGCTGGAGCGAACGCAAGGACGGC
GAGACGAAGAAGTTCGAAGGCATCTTCTCCAAGCTCGATGTTTCGCGCGTCGAAGCCGAGAACATGGTGGTGCAGGCCCG
TCTTCTGGCAGGCTGGATCACCGCTGAAGAGCTTGCTTCCGAACAGGAAGTCGAGGCGGAAGCTGCCGAGGAGGCGGATA
CCGCCGAACAGGAATGA

Upstream 100 bases:

>100_bases
AAACCAGAACGACGAAAACGAAGCAGACGAAGACTAATTCCAACGTAACGCCCGGAAAACGGGCGATCCCCGCAATTATG
CGACTTACGGAGATAAAAAA

Downstream 100 bases:

>100_bases
CATCGCAGGCGCATGAGCCGGACGATCTGCCCGAGACTGACGATGACGGGCGGATCAAGGGCGACGTCAATGGACGTATG
TGCATTGTGACACGGCAGAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSETNIRADINSKTGEIRLQRLLEVVEAAEDYSTQI
PLELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRIGEIVNGTVKRVEYGNVIVDLG
RGEGIIRRDEMIPRENMRYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIQIKSVARDPGSRAKI
AVISNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSQEPASFIVNALQPAEVAKVVLDEESERIEVVVPDEQLSL
AIGRRGQNVRLASQLTGWDIDIMTEQEESERRQKEFNERTALFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEITSID
GFDEDTADEIQTRAREYLERLEAEMDAKRKELGVTDELRQIDGLTSQMMVALGEDGIKTIEDFAGCAADDLVGWSERKDG
ETKKFEGIFSKLDVSRVEAENMVVQARLLAGWITAEELASEQEVEAEAAEEADTAEQE

Sequences:

>Translated_538_residues
MAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSETNIRADINSKTGEIRLQRLLEVVEAAEDYSTQI
PLELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRIGEIVNGTVKRVEYGNVIVDLG
RGEGIIRRDEMIPRENMRYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIQIKSVARDPGSRAKI
AVISNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSQEPASFIVNALQPAEVAKVVLDEESERIEVVVPDEQLSL
AIGRRGQNVRLASQLTGWDIDIMTEQEESERRQKEFNERTALFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEITSID
GFDEDTADEIQTRAREYLERLEAEMDAKRKELGVTDELRQIDGLTSQMMVALGEDGIKTIEDFAGCAADDLVGWSERKDG
ETKKFEGIFSKLDVSRVEAENMVVQARLLAGWITAEELASEQEVEAEAAEEADTAEQE
>Mature_537_residues
AVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSETNIRADINSKTGEIRLQRLLEVVEAAEDYSTQIP
LELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEFKDRIGEIVNGTVKRVEYGNVIVDLGR
GEGIIRRDEMIPRENMRYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIQIKSVARDPGSRAKIA
VISNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSQEPASFIVNALQPAEVAKVVLDEESERIEVVVPDEQLSLA
IGRRGQNVRLASQLTGWDIDIMTEQEESERRQKEFNERTALFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEITSIDG
FDEDTADEIQTRAREYLERLEAEMDAKRKELGVTDELRQIDGLTSQMMVALGEDGIKTIEDFAGCAADDLVGWSERKDGE
TKKFEGIFSKLDVSRVEAENMVVQARLLAGWITAEELASEQEVEAEAAEEADTAEQE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=476, Percent_Identity=43.4873949579832, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 60261; Mature: 60129

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSETNIRADINSKTGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
IRLQRLLEVVEAAEDYSTQIPLELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
QKVREAERDRQYDEFKDRIGEIVNGTVKRVEYGNVIVDLGRGEGIIRRDEMIPRENMRYG
HHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEECCEEEECCCCCCEEECCCCCCCCCCCCH
DRVRAYVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIQIKSVARDPGSRAKI
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEE
AVISNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSQEPASFIVNALQPAEVAKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VLDEESERIEVVVPDEQLSLAIGRRGQNVRLASQLTGWDIDIMTEQEESERRQKEFNERT
HHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ALFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEITSIDGFDEDTADEIQTRAREYLER
HHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LEAEMDAKRKELGVTDELRQIDGLTSQMMVALGEDGIKTIEDFAGCAADDLVGWSERKDG
HHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCC
ETKKFEGIFSKLDVSRVEAENMVVQARLLAGWITAEELASEQEVEAEAAEEADTAEQE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVSANRLELLQIADAVAREKVIDREIVLAAMADAIQKAARSRYGSETNIRADINSKTGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
IRLQRLLEVVEAAEDYSTQIPLELARDRNPDAKLGDFIADPLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
QKVREAERDRQYDEFKDRIGEIVNGTVKRVEYGNVIVDLGRGEGIIRRDEMIPRENMRYG
HHHHHHHHCCHHHHHHHHHHHHHCCHHHHEEECCEEEECCCCCCEEECCCCCCCCCCCCH
DRVRAYVYDVRREQRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIQIKSVARDPGSRAKI
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEE
AVISNDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSQEPASFIVNALQPAEVAKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VLDEESERIEVVVPDEQLSLAIGRRGQNVRLASQLTGWDIDIMTEQEESERRQKEFNERT
HHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ALFMEALDVDEMVGQVLASEGFAQVEELAYVDLDEITSIDGFDEDTADEIQTRAREYLER
HHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LEAEMDAKRKELGVTDELRQIDGLTSQMMVALGEDGIKTIEDFAGCAADDLVGWSERKDG
HHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCC
ETKKFEGIFSKLDVSRVEAENMVVQARLLAGWITAEELASEQEVEAEAAEEADTAEQE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]