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The map label for this gene is livK [C]

Identifier: 158423733

GI number: 158423733

Start: 2425904

End: 2427127

Strand: Direct

Name: livK [C]

Synonym: AZC_2109

Alternate gene names: 158423733

Gene position: 2425904-2427127 (Clockwise)

Preceding gene: 158423732

Following gene: 158423734

Centisome position: 45.18

GC content: 67.97

Gene sequence:

>1224_bases
ATGACCATCTGGACGACGCCTCTCGGCCGTCGTGCGGTCCTGAAGGGCAGCACGGCAGCGCTTGCGACCCTCGCCATGCC
CGCCATCGTGCGCGGTGCTGAGCCCGCGCCCATCAAGGTGGGCGTGCTCCAGCCGGTCACCGGCGCGCTGGCCATGGATG
GTGAATATGGCCGCACCGGTGCCGAATTCGCCATCGCCGCCGTGAACGCGGCGGGCGGCATCAAGTCGCTCGGCGGCGCC
AAGCTTGAGATGGTGTTCGGCGATGCCCGATCGAACCCCGAGGCGGGCACGCAGGAAGTGGAGCGGATGCAGGGCGAGGG
CGTGGTGGCCGTCGTCGGCGGCTTCGCCAGCGGCATCTGCCTTGCCGCCAGCCAGACCGCTGCGCGCTATGACCTGCCCT
ATCTCGTGGACGTGGGCGTCTCGGATCAGATCGTCCAGCGCGGCCTCAAGAACACCTTCCGCTTCGGCCCCGGCTTCGGC
GTCTGCACACGGTCGGCCCTCGATAATCTCGCCCGCATCAACGAGGCCGCCGGCAAGCCGGCCAAGACCGTCGTGCTGGT
GCACGAGGATGGCTTGTTCGGCAGTGGCCTCGCCAAGGTGATGCAGGCGGAACTGCCCAAGCTCGGCTTCGAGATCCTCG
ACACCATTCCGGTGCCGACCCCCTCGCGTGATCTGTCCAACGTGGTGCTGCGCCTGCGCTCGCTGAACCCGGACATCGTG
ATGCCGAGCACCTACTACGGCGAGACGGTGCTGCTCGCCCGCACCATGCAGCAGCAGCGCGTGCGGCCGAAGGCGGTCTA
TGGCGTGCTGAACGGCGCCGCCTCCAACATGCGCTTCGTGAAGGAATTCCCCGAAGCCGCCGACAAGATCATGGACTGCA
ATCACTGGTACGACCCGCGCAAGGCAGGCGCCCAGGCGCTGCTGAAGGCGGTGGCGGATGCCGGCAAGGGCTGGAACTAC
AACCTGCCCATGAACTATTCCTGCGTCGGCCTGCTGGCGGACGCGCTGGAGCGCGCCGGCTCCACGGACCGCGCCAAGGT
GATCGACGCGCTTGCCGCCTCCACCTGGGCGGACCATGTGATGCCCTACGGCCCCACCAAGTTCGTGGACGGCCAGAACC
AGGGCGCGGCTCCGGTCTCGCTTCAGGTGCAGAACGGTGCCATCCGCGTCGTCTATCCGGAGGCCTTCTCCGACGCCAAG
GCCATCTTCCCGGTCCGGTCCTGA

Upstream 100 bases:

>100_bases
TCGCCGTTCCGCGCGGTCTCGAAACCAAGGCCGCAGCCAAAGCACCGGCCAATCAGAAAAGACGCCCGAAAAGGGCCGAT
CCTCAGAGGAGAACTGCGAG

Downstream 100 bases:

>100_bases
TCCCCGTGGCGCCGGAGCGATCCGGCGCCGTCCCCTCGATGCACCGGCCCATGGGCCGGTCAGCGAGCGCTGGATGATTT
GATGTATTCGCTCCCCATCC

Product: branched-chain amino acid transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTIWTTPLGRRAVLKGSTAALATLAMPAIVRGAEPAPIKVGVLQPVTGALAMDGEYGRTGAEFAIAAVNAAGGIKSLGGA
KLEMVFGDARSNPEAGTQEVERMQGEGVVAVVGGFASGICLAASQTAARYDLPYLVDVGVSDQIVQRGLKNTFRFGPGFG
VCTRSALDNLARINEAAGKPAKTVVLVHEDGLFGSGLAKVMQAELPKLGFEILDTIPVPTPSRDLSNVVLRLRSLNPDIV
MPSTYYGETVLLARTMQQQRVRPKAVYGVLNGAASNMRFVKEFPEAADKIMDCNHWYDPRKAGAQALLKAVADAGKGWNY
NLPMNYSCVGLLADALERAGSTDRAKVIDALAASTWADHVMPYGPTKFVDGQNQGAAPVSLQVQNGAIRVVYPEAFSDAK
AIFPVRS

Sequences:

>Translated_407_residues
MTIWTTPLGRRAVLKGSTAALATLAMPAIVRGAEPAPIKVGVLQPVTGALAMDGEYGRTGAEFAIAAVNAAGGIKSLGGA
KLEMVFGDARSNPEAGTQEVERMQGEGVVAVVGGFASGICLAASQTAARYDLPYLVDVGVSDQIVQRGLKNTFRFGPGFG
VCTRSALDNLARINEAAGKPAKTVVLVHEDGLFGSGLAKVMQAELPKLGFEILDTIPVPTPSRDLSNVVLRLRSLNPDIV
MPSTYYGETVLLARTMQQQRVRPKAVYGVLNGAASNMRFVKEFPEAADKIMDCNHWYDPRKAGAQALLKAVADAGKGWNY
NLPMNYSCVGLLADALERAGSTDRAKVIDALAASTWADHVMPYGPTKFVDGQNQGAAPVSLQVQNGAIRVVYPEAFSDAK
AIFPVRS
>Mature_406_residues
TIWTTPLGRRAVLKGSTAALATLAMPAIVRGAEPAPIKVGVLQPVTGALAMDGEYGRTGAEFAIAAVNAAGGIKSLGGAK
LEMVFGDARSNPEAGTQEVERMQGEGVVAVVGGFASGICLAASQTAARYDLPYLVDVGVSDQIVQRGLKNTFRFGPGFGV
CTRSALDNLARINEAAGKPAKTVVLVHEDGLFGSGLAKVMQAELPKLGFEILDTIPVPTPSRDLSNVVLRLRSLNPDIVM
PSTYYGETVLLARTMQQQRVRPKAVYGVLNGAASNMRFVKEFPEAADKIMDCNHWYDPRKAGAQALLKAVADAGKGWNYN
LPMNYSCVGLLADALERAGSTDRAKVIDALAASTWADHVMPYGPTKFVDGQNQGAAPVSLQVQNGAIRVVYPEAFSDAKA
IFPVRS

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=342, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994941, Length=245, Percent_Identity=27.3469387755102, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42992; Mature: 42861

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIWTTPLGRRAVLKGSTAALATLAMPAIVRGAEPAPIKVGVLQPVTGALAMDGEYGRTG
CEEECCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCC
AEFAIAAVNAAGGIKSLGGAKLEMVFGDARSNPEAGTQEVERMQGEGVVAVVGGFASGIC
CCEEEEEEECCCCHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCE
LAASQTAARYDLPYLVDVGVSDQIVQRGLKNTFRFGPGFGVCTRSALDNLARINEAAGKP
EECCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
AKTVVLVHEDGLFGSGLAKVMQAELPKLGFEILDTIPVPTPSRDLSNVVLRLRSLNPDIV
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
MPSTYYGETVLLARTMQQQRVRPKAVYGVLNGAASNMRFVKEFPEAADKIMDCNHWYDPR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
KAGAQALLKAVADAGKGWNYNLPMNYSCVGLLADALERAGSTDRAKVIDALAASTWADHV
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MPYGPTKFVDGQNQGAAPVSLQVQNGAIRVVYPEAFSDAKAIFPVRS
CCCCCCEEECCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIWTTPLGRRAVLKGSTAALATLAMPAIVRGAEPAPIKVGVLQPVTGALAMDGEYGRTG
EEECCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCC
AEFAIAAVNAAGGIKSLGGAKLEMVFGDARSNPEAGTQEVERMQGEGVVAVVGGFASGIC
CCEEEEEEECCCCHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCE
LAASQTAARYDLPYLVDVGVSDQIVQRGLKNTFRFGPGFGVCTRSALDNLARINEAAGKP
EECCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
AKTVVLVHEDGLFGSGLAKVMQAELPKLGFEILDTIPVPTPSRDLSNVVLRLRSLNPDIV
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
MPSTYYGETVLLARTMQQQRVRPKAVYGVLNGAASNMRFVKEFPEAADKIMDCNHWYDPR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
KAGAQALLKAVADAGKGWNYNLPMNYSCVGLLADALERAGSTDRAKVIDALAASTWADHV
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MPYGPTKFVDGQNQGAAPVSLQVQNGAIRVVYPEAFSDAKAIFPVRS
CCCCCCEEECCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA