BacMap

Definition	Shewanella pealeana ATCC 700345 chromosome, complete genome.
Accession	NC_009901
Length	5,174,581

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The map label for this gene is paaF [H]

Identifier: 157963381

GI number: 157963381

Start: 4354179

End: 4354916

Strand: Direct

Name: paaF [H]

Synonym: Spea_3567

Alternate gene names: 157963381

Gene position: 4354179-4354916 (Clockwise)

Preceding gene: 157963377

Following gene: 157963387

Centisome position: 84.15

GC content: 45.53

Gene sequence:

>738_bases
ATGAGCACGATTCAGGTTAAGGACGATAATGGGGTACGTATCATTACGTTTAATCGTCCGGATAAGCGTAACGCGCTTAA
CCTACAAATGTATACACAACTAACAGAATACCTTATCCAAGGTGAGTCGGACAATGAAATTCGAGCCTTTATGTTTCGTG
GCCAAGATGATTGTTTTACTTCAGGTAATGATGTCGCAGACTTCCTACAAAATAGTGGCTTAGGAGCCCAACATCCTGCT
TTTCGTTTCCTCTTCTGCCTACTCGATCTACAAAAGCCTGTTGTGGCCGCCGTCACTGGCGCCGCCGTCGGCATTGGCAC
CACCATGCTACTGCATTGCGATTTAGTTTATGCGGACCAGAGCGCCAAGTTTCAACTACCATTCGTGCAGCTTGCATTAG
TCCCTGAAGCGGGAGCTAGCCTATTGCTACCTCAGGTTATTGGTCAGGCAAAAGCCAGTGAGCTATTAATGTTAGGTGAG
AGCTTTAATGCTGTTCAAGCCCAAAGCTATGGACTTATTAATGACGTTATCGACAGCGAGACTATTTTTGAGTACGCCTT
GAGTCGTGCACATCAGTTAGCGACGCTGCCACCCATATCGCTACAAACTACCAAAAAATTAATTCGCTATAACAGGGAAG
AAGTGCGAGCGCAGATGAAGAAAGAGCTTATCGAATTTGAAGCCAGGCTGGCAAGTGACGAAGCCAAAGCAAGATTTGCT
GCGTTTTTAAAACGTTAA

Upstream 100 bases:

>100_bases
ATAAACCGCTTAATAGCGCCAAGTTAAACAGTTGTTTGAATATTAGCTTCAATGTCTTAAACTCCATGTAAACCCAATTC
GTACAACGAGGATATAACGA

Downstream 100 bases:

>100_bases
TACAGCCTCGTTAAATACAGTCCCATTAAAGACTGTCTCATTAAAGACATTGAGTGCGGATGCTAAGCCTTGCTTGACAG
CATCCGCATTTTATCGCGAT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSTIQVKDDNGVRIITFNRPDKRNALNLQMYTQLTEYLIQGESDNEIRAFMFRGQDDCFTSGNDVADFLQNSGLGAQHPA
FRFLFCLLDLQKPVVAAVTGAAVGIGTTMLLHCDLVYADQSAKFQLPFVQLALVPEAGASLLLPQVIGQAKASELLMLGE
SFNAVQAQSYGLINDVIDSETIFEYALSRAHQLATLPPISLQTTKKLIRYNREEVRAQMKKELIEFEARLASDEAKARFA
AFLKR

Sequences:

>Translated_245_residues
MSTIQVKDDNGVRIITFNRPDKRNALNLQMYTQLTEYLIQGESDNEIRAFMFRGQDDCFTSGNDVADFLQNSGLGAQHPA
FRFLFCLLDLQKPVVAAVTGAAVGIGTTMLLHCDLVYADQSAKFQLPFVQLALVPEAGASLLLPQVIGQAKASELLMLGE
SFNAVQAQSYGLINDVIDSETIFEYALSRAHQLATLPPISLQTTKKLIRYNREEVRAQMKKELIEFEARLASDEAKARFA
AFLKR
>Mature_244_residues
STIQVKDDNGVRIITFNRPDKRNALNLQMYTQLTEYLIQGESDNEIRAFMFRGQDDCFTSGNDVADFLQNSGLGAQHPAF
RFLFCLLDLQKPVVAAVTGAAVGIGTTMLLHCDLVYADQSAKFQLPFVQLALVPEAGASLLLPQVIGQAKASELLMLGES
FNAVQAQSYGLINDVIDSETIFEYALSRAHQLATLPPISLQTTKKLIRYNREEVRAQMKKELIEFEARLASDEAKARFAA
FLKR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=33.8645418326693, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=33.8645418326693, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=33.8645418326693, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI25453481, Length=212, Percent_Identity=27.8301886792453, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI51538573, Length=212, Percent_Identity=27.8301886792453, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=27.8301886792453, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=27.8301886792453, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=27.9352226720648, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4757968, Length=212, Percent_Identity=27.3584905660377, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI49355787, Length=212, Percent_Identity=27.3584905660377, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI221136756, Length=212, Percent_Identity=25, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI221136753, Length=212, Percent_Identity=25, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI221307494, Length=162, Percent_Identity=29.0123456790123, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=220, Percent_Identity=30, Blast_Score=94, Evalue=7e-20,
Organism=Homo sapiens, GI116235487, Length=214, Percent_Identity=26.1682242990654, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI31542718, Length=198, Percent_Identity=25.2525252525253, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=26.2222222222222, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=26.2222222222222, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=215, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=32.7935222672065, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787660, Length=209, Percent_Identity=38.2775119617225, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI221142681, Length=202, Percent_Identity=28.7128712871287, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=27.1739130434783, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788597, Length=213, Percent_Identity=26.2910798122066, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=214, Percent_Identity=24.7663551401869, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17549921, Length=246, Percent_Identity=30.0813008130081, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=28.4584980237154, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=25.5144032921811, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=33.6585365853659, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=27.7551020408163, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=240, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=233, Percent_Identity=24.4635193133047, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=186, Percent_Identity=25.2688172043011, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24654903, Length=205, Percent_Identity=31.219512195122, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=28.5123966942149, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=28.5123966942149, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653139, Length=207, Percent_Identity=30.9178743961353, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=26.1467889908257, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24650670, Length=252, Percent_Identity=24.2063492063492, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=26.4150943396226, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=183, Percent_Identity=23.4972677595628, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27241; Mature: 27109

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIQVKDDNGVRIITFNRPDKRNALNLQMYTQLTEYLIQGESDNEIRAFMFRGQDDCFT
CCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SGNDVADFLQNSGLGAQHPAFRFLFCLLDLQKPVVAAVTGAAVGIGTTMLLHCDLVYADQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEECC
SAKFQLPFVQLALVPEAGASLLLPQVIGQAKASELLMLGESFNAVQAQSYGLINDVIDSE
CCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCHHHHHHCHH
TIFEYALSRAHQLATLPPISLQTTKKLIRYNREEVRAQMKKELIEFEARLASDEAKARFA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKR
HHHCC
>Mature Secondary Structure 
STIQVKDDNGVRIITFNRPDKRNALNLQMYTQLTEYLIQGESDNEIRAFMFRGQDDCFT
CEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SGNDVADFLQNSGLGAQHPAFRFLFCLLDLQKPVVAAVTGAAVGIGTTMLLHCDLVYADQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEECC
SAKFQLPFVQLALVPEAGASLLLPQVIGQAKASELLMLGESFNAVQAQSYGLINDVIDSE
CCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCHHHHHHCHH
TIFEYALSRAHQLATLPPISLQTTKKLIRYNREEVRAQMKKELIEFEARLASDEAKARFA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AFLKR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]