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The map label for this gene is kefA [H]

Identifier: 157963205

GI number: 157963205

Start: 4143630

End: 4146215

Strand: Direct

Name: kefA [H]

Synonym: Spea_3390

Alternate gene names: 157963205

Gene position: 4143630-4146215 (Clockwise)

Preceding gene: 157963204

Following gene: 157963206

Centisome position: 80.08

GC content: 42.27

Gene sequence:

>2586_bases
TTGATTGAATTACAAGATAAATTAACCCGAGTCGAGCAGGCCTCAGTTAAAACACTCATTGAACAAGCGAGGCAGGTTAG
CGAGGAGGCGCTAGGAGATACGGAGGAGCAACACCAAGTAGAGCTAGTCAAAGCTTACACTTTAGAACTAGAAGAGGTAC
TGGATAAGATAGATAAAACGCGAATTATGTATCAAAAAGTTGAGTCTGAGCAACGCAGTTTAGCGGATGACCAACGCTTG
ATAAAAGATAACTTAGCTTGGTTACGCGATAGCACGGTTTTTGGTGCTTCCATCCGAGCACAGTTACAAAGGTTACCCTC
CAAAATAGACACGCAATATATTCCCGATGAAATTGCCAAAGCCCACATTCGAAAATACGAAATAGGCCAATTAAAAGATG
ATGCTGAGCAACCAAGTGCAACTTCTAGAGATAAAAAAAACGAAGAAGGTCCAGACAAGTTAGCCTCGTTATTAAATAAA
TTATTAGACCAATTAACGAAAGATTATGACAAGCTAATTTCAGCACTGAGTCAGCTAAAGTTGGTCAAGGATCAATATGC
TCAAACAGTGGAGAAGGCACGTAGTTTTTTAAAGCAGCAACAGCTATGGACACGCAGTAACGTGCCCATATGGCAACACC
TAACTGACTTCAATACTGTGACCTGGTTCGGCTCCAAGACACCAATTAAAAATATGTTAGATGAGCTCCCACAAGGACTG
TCTGTATCGCTGTTAACTGTATTTGCTTTGTACACGATTCTTTTTATCTATAGCACTAGTCGGCTGAATCAATATGCCGC
TCAGCGTAAGGATGAGCTTCAAAAAGTATTTGGTCACCCGCTGAAAGATCGATTTCATAATACTGTTTTGTTATTTTTAA
TCGCCATTATACGCGCGCTTATTCTGCCTATTTGGTATGTAATAACCGCTTGGGGACTGTATTGGTTTTGGCCAGTTACG
ACCTCAAATGAGCTCAGTACACTGATCTTTGCCAGCGCATCTGGCGTGTTTGTCGTCGAACTCATCCACGCCTTAAGCTA
TAAACAAGGTGTTCTCGCACTCCATCTAAACTGGCCAGAGCAAATTTGCTCATACCTACATAAAGGAAGTCAACGGATAC
TCTGGCCATTTGTCATTTTGCTACTAAGTATTTTTGCTGCAGAACTGGTTTCAGGGATCCATGAAGCGGAAATATCTCGC
GTATTATTCCTATTACTAATGGGAGTAATGATGGCTGTCTTTATTTCACTGCTGAAAGAAAAACATCTCCCCTCAACTTT
ACCGTCCCCACTTAATAGAGGCTTCTCCCTCTATCTATTTCGTTTCATCGTTATCGGTTCGTTTATTACTATTATCGTTA
TGGCCGTTATGGGGCTCTATATTGCTTCATGGATCTTACTTATCTATCAACAGTTAACCATTTACGTAGTGTTAGCAGTG
CTGTTTACCTATCAAATGGGAGAACGCTGGCTAAAGATAGAGCATCGGCAGCTCAGCTATCAGCGGCTGCTAGCGCGGCG
TGAAGAGCTCATTGCTCAACAACAAGAGCAAGCAGATGAGTCACCCGAAATTACTGAATTAAGGGAAACTATTCCTGATG
TAGAAGAGCGCAGTTTAGCGAGTGAACAAGTTAGTGAACAGTCACTAACACTGTTGCGTGGCTTGTCCTTAATTGGCTTA
GTGGCTGCAGTGTTAACCTTATGGTCAAGCGCCTTAGAAATGACCAGTTGGTTAGATGCCATTGTGGTGTGGCAAGTAAA
TGAAATGACTCAAACCGGCGTAACCCTGGTGGATGTGACTCTGCAATCATTGATTTATGCAGTGGTCACATTAATCGTAA
CCTTTGTTGGTGTACGAAACTTACCAGGTATTTTGGAACTGTTAATCCTGCGTCGTCTCGACTTAGCGCCTGGCGCCGGC
TATGCGGTTACCACTCTATTGCGCTATGTAATTTTAATGGCAGGCTTAATGGGTGCGTTTTCCATATTAGGCTTTCAATG
GTCTAAACTGCAATGGCTAGTTGCCGCATTCGGTGTAGGCTTAGGATTTGGCTTACAAGAAATTTTCGCCAACTTTATTT
CCGGTTTAATTTTACTGTTTGAACGACCTATCCGTATTGGGGACATTGTTACGATTAATGATTTATCGGGAACGGTGAGC
AAAATTCAAACACGCGCCACCACCATTATTGATTGGGACAACAAAGAAATCGTGGTGCCCAATAAAACCTTTATTACAGA
AAAGCTCATTAACTGGTCGCTAACTAGCCCTATTACCAGAATCGTGATCCCTATTGGTGTCGCTTATGGTTCTGATATTG
ATAAGGTGGAGAGCTTACTGTATCAAATCGCGGATGAGCACCCACTCGTACTACCTGATCCGCAACCTTCTGTGGTGTTC
TTATCTTTTGGAGCTAGCAGTCTCGACTTTGAATTAAGAGCCCATATCACTGCGATTGAACTACGCCTATCAACGGTTCA
TATCATCAACAAAAATATTAATCGCTTGTTTCGAGAACACGAGATTGAAATTGCCTTTCCGCAAATGGATGTGCATGTTC
GTGATTTGCCGCCAAAAGATACATGA

Upstream 100 bases:

>100_bases
AAGTGACCTCTGCTCAGTTACAGAGCTTAGATGAACGCACTGAATTACTGATTTAGAGCAGCAATTACTGAACCAAAAAA
TTCAATTAGCCTCGCCAATT

Downstream 100 bases:

>100_bases
GTTTAATGTTTAGTGATGAGCTATTAATTGGCGATATCGATTCTGCAGGGAGAAAGACTTAAACAAAAATAAGCCTGCTA
CTTGCTGGTGATTTTACAAT

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MIELQDKLTRVEQASVKTLIEQARQVSEEALGDTEEQHQVELVKAYTLELEEVLDKIDKTRIMYQKVESEQRSLADDQRL
IKDNLAWLRDSTVFGASIRAQLQRLPSKIDTQYIPDEIAKAHIRKYEIGQLKDDAEQPSATSRDKKNEEGPDKLASLLNK
LLDQLTKDYDKLISALSQLKLVKDQYAQTVEKARSFLKQQQLWTRSNVPIWQHLTDFNTVTWFGSKTPIKNMLDELPQGL
SVSLLTVFALYTILFIYSTSRLNQYAAQRKDELQKVFGHPLKDRFHNTVLLFLIAIIRALILPIWYVITAWGLYWFWPVT
TSNELSTLIFASASGVFVVELIHALSYKQGVLALHLNWPEQICSYLHKGSQRILWPFVILLLSIFAAELVSGIHEAEISR
VLFLLLMGVMMAVFISLLKEKHLPSTLPSPLNRGFSLYLFRFIVIGSFITIIVMAVMGLYIASWILLIYQQLTIYVVLAV
LFTYQMGERWLKIEHRQLSYQRLLARREELIAQQQEQADESPEITELRETIPDVEERSLASEQVSEQSLTLLRGLSLIGL
VAAVLTLWSSALEMTSWLDAIVVWQVNEMTQTGVTLVDVTLQSLIYAVVTLIVTFVGVRNLPGILELLILRRLDLAPGAG
YAVTTLLRYVILMAGLMGAFSILGFQWSKLQWLVAAFGVGLGFGLQEIFANFISGLILLFERPIRIGDIVTINDLSGTVS
KIQTRATTIIDWDNKEIVVPNKTFITEKLINWSLTSPITRIVIPIGVAYGSDIDKVESLLYQIADEHPLVLPDPQPSVVF
LSFGASSLDFELRAHITAIELRLSTVHIINKNINRLFREHEIEIAFPQMDVHVRDLPPKDT

Sequences:

>Translated_861_residues
MIELQDKLTRVEQASVKTLIEQARQVSEEALGDTEEQHQVELVKAYTLELEEVLDKIDKTRIMYQKVESEQRSLADDQRL
IKDNLAWLRDSTVFGASIRAQLQRLPSKIDTQYIPDEIAKAHIRKYEIGQLKDDAEQPSATSRDKKNEEGPDKLASLLNK
LLDQLTKDYDKLISALSQLKLVKDQYAQTVEKARSFLKQQQLWTRSNVPIWQHLTDFNTVTWFGSKTPIKNMLDELPQGL
SVSLLTVFALYTILFIYSTSRLNQYAAQRKDELQKVFGHPLKDRFHNTVLLFLIAIIRALILPIWYVITAWGLYWFWPVT
TSNELSTLIFASASGVFVVELIHALSYKQGVLALHLNWPEQICSYLHKGSQRILWPFVILLLSIFAAELVSGIHEAEISR
VLFLLLMGVMMAVFISLLKEKHLPSTLPSPLNRGFSLYLFRFIVIGSFITIIVMAVMGLYIASWILLIYQQLTIYVVLAV
LFTYQMGERWLKIEHRQLSYQRLLARREELIAQQQEQADESPEITELRETIPDVEERSLASEQVSEQSLTLLRGLSLIGL
VAAVLTLWSSALEMTSWLDAIVVWQVNEMTQTGVTLVDVTLQSLIYAVVTLIVTFVGVRNLPGILELLILRRLDLAPGAG
YAVTTLLRYVILMAGLMGAFSILGFQWSKLQWLVAAFGVGLGFGLQEIFANFISGLILLFERPIRIGDIVTINDLSGTVS
KIQTRATTIIDWDNKEIVVPNKTFITEKLINWSLTSPITRIVIPIGVAYGSDIDKVESLLYQIADEHPLVLPDPQPSVVF
LSFGASSLDFELRAHITAIELRLSTVHIINKNINRLFREHEIEIAFPQMDVHVRDLPPKDT
>Mature_861_residues
MIELQDKLTRVEQASVKTLIEQARQVSEEALGDTEEQHQVELVKAYTLELEEVLDKIDKTRIMYQKVESEQRSLADDQRL
IKDNLAWLRDSTVFGASIRAQLQRLPSKIDTQYIPDEIAKAHIRKYEIGQLKDDAEQPSATSRDKKNEEGPDKLASLLNK
LLDQLTKDYDKLISALSQLKLVKDQYAQTVEKARSFLKQQQLWTRSNVPIWQHLTDFNTVTWFGSKTPIKNMLDELPQGL
SVSLLTVFALYTILFIYSTSRLNQYAAQRKDELQKVFGHPLKDRFHNTVLLFLIAIIRALILPIWYVITAWGLYWFWPVT
TSNELSTLIFASASGVFVVELIHALSYKQGVLALHLNWPEQICSYLHKGSQRILWPFVILLLSIFAAELVSGIHEAEISR
VLFLLLMGVMMAVFISLLKEKHLPSTLPSPLNRGFSLYLFRFIVIGSFITIIVMAVMGLYIASWILLIYQQLTIYVVLAV
LFTYQMGERWLKIEHRQLSYQRLLARREELIAQQQEQADESPEITELRETIPDVEERSLASEQVSEQSLTLLRGLSLIGL
VAAVLTLWSSALEMTSWLDAIVVWQVNEMTQTGVTLVDVTLQSLIYAVVTLIVTFVGVRNLPGILELLILRRLDLAPGAG
YAVTTLLRYVILMAGLMGAFSILGFQWSKLQWLVAAFGVGLGFGLQEIFANFISGLILLFERPIRIGDIVTINDLSGTVS
KIQTRATTIIDWDNKEIVVPNKTFITEKLINWSLTSPITRIVIPIGVAYGSDIDKVESLLYQIADEHPLVLPDPQPSVVF
LSFGASSLDFELRAHITAIELRLSTVHIINKNINRLFREHEIEIAFPQMDVHVRDLPPKDT

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=826, Percent_Identity=34.6246973365617, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI2367355, Length=837, Percent_Identity=30.8243727598566, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789291, Length=253, Percent_Identity=31.6205533596838, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787591, Length=204, Percent_Identity=25.9803921568627, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 97866; Mature: 97866

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEECCCCCEEEECCCCCCC
>Mature Secondary Structure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CCEEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]