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The map label for this gene is pleD [H]

Identifier: 157963009

GI number: 157963009

Start: 3913143

End: 3915026

Strand: Direct

Name: pleD [H]

Synonym: Spea_3192

Alternate gene names: 157963009

Gene position: 3913143-3915026 (Clockwise)

Preceding gene: 157963003

Following gene: 157963010

Centisome position: 75.62

GC content: 44.43

Gene sequence:

>1884_bases
ATGCCTAAGCTAATTCAGTTATTACTCATCTCCCTAGCTTTTATTATGGCCTCGGTCAGCCATTATGCTGTTTCTGCCAC
AACGGATCTCCAACGTGCAGATGAGCTATTCGACTTGCTCGATAGTGGTGTTGTTGAGAAGAACCTCGAAAAGAAGCAAT
ACCTCGAAGAGCTGACCCCCTTAGTGCCAAAAAATGACCTAGCGAGGCAATTACGTTTAGCTCGAACCATCTGCTGGTCA
TATAACATGCAAGATGATGCTGAAATTGCTCAAGCCTTGTTATTTGCTAAACAAGCCTTAACGCGTCCGGAGTTAACCAA
TTTCCCTGATGATAAATTGGACTTAGAGCTTTGCTATGCTTGGTTTTCTGAGCAAAAAGGCGATGTCGATACAGCGCTGA
GTTATTACAATCGACTGGTCATTGACGCCTACAAGTTAGAAAATTTGCGCCTAATGGCTGACGTTCGAATGATGCGCGGC
TATTTAACTTCGTTCCAAGGCAACTATACCCAAGGCCTAGAGGACTTAATGACGGCGCAAACCCTCTATAAAAGTTTAAA
TGTCACCGTTTGGGTACAGCATAGCCTCTATGAAATCGCGACGATTTATCGCCGCTTAGGTGATCAAGAAAATGCTATCC
GTTATTTCAACCAGTTGCATAAGAGCCACATCGACAACCAAGAGTTTGACAAGGCTAACGTGGTGATCTCATCAATCGCT
ATTGCTGAAGAGGAGCGCAAGAGCTTTACTCAAGCTAAAAAACTTTTCTTAGAAAGTTACCACTACTGGAACACTAACCA
AAATGGGTTTAAGAAAGCCGGCGTAGCCGTGAACATGGCTGGAACCTTAATTAAACTTGGAGAAATCAACCAAGCCCTTA
AGTACCTAGATGAGTCAGTTCCTTTTATCACTCCATCTGCGGAAGGCTTCTATAACTATATGCAGCTGTTTTATGCCCAG
ATATACTTGGAGCTAGGGCAGTTCGAACAAGCCCACAAAAGCATAGCCTTAGCCCGCGCAGCATTTGAGCGAGTCAAGAG
TTTGCGCGGACTGGTGCAGGTGCAACTTGTAGAGAGCCAGATATTTCTTATGCAAGATAACTGGCAAGCAGCCTATGAGT
CTCTATATAAGTATATGGAGCTGCATATGGAGCTTGATAAAAAGCTATTCTCTCGCTACAACACCGAAATGCGAACCCGT
TTTAATGCCGAGCAGATAGAAGCCGAGAATCGCCACCTCGTTGAAAATCATAAGCTCAAAGAGCTTGAGCTAGCCATGCT
GGAGCAAAATAAAGTTCAGCAATGGATTATCATTTTTATGGGCAGCATAATCCTTATTATCATCTCGACTTTCACCTACA
AGCAGTCGCAGAAGAATAAATTACTGTCGGCGTTAGCCCTGACTGACGATCTCACTCAACTGCCTAATCGACGCTACATC
TACACCCAAGCGCAGGACTACTTTGAGAGCGCAATAAAAAACCAGCAGGCTTTATCCTGTATTAGTTTCGATGCTGACTA
TTTCAAACTCATTAATGACAGATATGGACACGAAGTTGGCGACGAGACCCTAAAGTTACTCGCTGATACCTGTAGAGCAG
TACTCGGAACTCAATATGAAGCGGCGCGAGTGGGGGGCGAAGAGTTTCTGATCTTACTGCCGCATACCGATAAGGCTCAA
GCCCTTAAGGTTGCCCAACACTTAGTTAATCAGGTTCGCAATGCTGACTTTAGCCCTTTCCCTGAAGGGTTCTCGATAAC
AATAAGTGCCGGTGTTTCAAGCCTTAGCGAAACTGACGATAAGCTTTCCCAACTGCTGAAACGAGCCGATGAGGCACTTT
ACTTAGCCAAAAAACAAGGCCGTGACCAAGTTAAGATGAATTAA

Upstream 100 bases:

>100_bases
ATTTTAAGCACTCAACATATAAAAACTTAGTTAACAAAAACCCTTATCGTGTATTCTAATGGCCGAATATAAAAAATTTT
GATACCAAAAATAAACGTAA

Downstream 100 bases:

>100_bases
TAGTCTGTCACTTTGGTTTTCAAGTCTGGCTTTCATTGCAGTAAATAGTCGGTTTTAGGCGAAAGATTTATAACTATCAG
CTTTGGCTATATTAAGGACC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MPKLIQLLLISLAFIMASVSHYAVSATTDLQRADELFDLLDSGVVEKNLEKKQYLEELTPLVPKNDLARQLRLARTICWS
YNMQDDAEIAQALLFAKQALTRPELTNFPDDKLDLELCYAWFSEQKGDVDTALSYYNRLVIDAYKLENLRLMADVRMMRG
YLTSFQGNYTQGLEDLMTAQTLYKSLNVTVWVQHSLYEIATIYRRLGDQENAIRYFNQLHKSHIDNQEFDKANVVISSIA
IAEEERKSFTQAKKLFLESYHYWNTNQNGFKKAGVAVNMAGTLIKLGEINQALKYLDESVPFITPSAEGFYNYMQLFYAQ
IYLELGQFEQAHKSIALARAAFERVKSLRGLVQVQLVESQIFLMQDNWQAAYESLYKYMELHMELDKKLFSRYNTEMRTR
FNAEQIEAENRHLVENHKLKELELAMLEQNKVQQWIIIFMGSIILIIISTFTYKQSQKNKLLSALALTDDLTQLPNRRYI
YTQAQDYFESAIKNQQALSCISFDADYFKLINDRYGHEVGDETLKLLADTCRAVLGTQYEAARVGGEEFLILLPHTDKAQ
ALKVAQHLVNQVRNADFSPFPEGFSITISAGVSSLSETDDKLSQLLKRADEALYLAKKQGRDQVKMN

Sequences:

>Translated_627_residues
MPKLIQLLLISLAFIMASVSHYAVSATTDLQRADELFDLLDSGVVEKNLEKKQYLEELTPLVPKNDLARQLRLARTICWS
YNMQDDAEIAQALLFAKQALTRPELTNFPDDKLDLELCYAWFSEQKGDVDTALSYYNRLVIDAYKLENLRLMADVRMMRG
YLTSFQGNYTQGLEDLMTAQTLYKSLNVTVWVQHSLYEIATIYRRLGDQENAIRYFNQLHKSHIDNQEFDKANVVISSIA
IAEEERKSFTQAKKLFLESYHYWNTNQNGFKKAGVAVNMAGTLIKLGEINQALKYLDESVPFITPSAEGFYNYMQLFYAQ
IYLELGQFEQAHKSIALARAAFERVKSLRGLVQVQLVESQIFLMQDNWQAAYESLYKYMELHMELDKKLFSRYNTEMRTR
FNAEQIEAENRHLVENHKLKELELAMLEQNKVQQWIIIFMGSIILIIISTFTYKQSQKNKLLSALALTDDLTQLPNRRYI
YTQAQDYFESAIKNQQALSCISFDADYFKLINDRYGHEVGDETLKLLADTCRAVLGTQYEAARVGGEEFLILLPHTDKAQ
ALKVAQHLVNQVRNADFSPFPEGFSITISAGVSSLSETDDKLSQLLKRADEALYLAKKQGRDQVKMN
>Mature_626_residues
PKLIQLLLISLAFIMASVSHYAVSATTDLQRADELFDLLDSGVVEKNLEKKQYLEELTPLVPKNDLARQLRLARTICWSY
NMQDDAEIAQALLFAKQALTRPELTNFPDDKLDLELCYAWFSEQKGDVDTALSYYNRLVIDAYKLENLRLMADVRMMRGY
LTSFQGNYTQGLEDLMTAQTLYKSLNVTVWVQHSLYEIATIYRRLGDQENAIRYFNQLHKSHIDNQEFDKANVVISSIAI
AEEERKSFTQAKKLFLESYHYWNTNQNGFKKAGVAVNMAGTLIKLGEINQALKYLDESVPFITPSAEGFYNYMQLFYAQI
YLELGQFEQAHKSIALARAAFERVKSLRGLVQVQLVESQIFLMQDNWQAAYESLYKYMELHMELDKKLFSRYNTEMRTRF
NAEQIEAENRHLVENHKLKELELAMLEQNKVQQWIIIFMGSIILIIISTFTYKQSQKNKLLSALALTDDLTQLPNRRYIY
TQAQDYFESAIKNQQALSCISFDADYFKLINDRYGHEVGDETLKLLADTCRAVLGTQYEAARVGGEEFLILLPHTDKAQA
LKVAQHLVNQVRNADFSPFPEGFSITISAGVSSLSETDDKLSQLLKRADEALYLAKKQGRDQVKMN

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=188, Percent_Identity=36.7021276595745, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=224, Percent_Identity=34.375, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.0578034682081, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=31.4465408805031, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=33.5329341317365, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787262, Length=270, Percent_Identity=27.4074074074074, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=216, Percent_Identity=28.2407407407407, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788085, Length=188, Percent_Identity=28.7234042553192, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=194, Percent_Identity=30.9278350515464, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=152, Percent_Identity=27.6315789473684, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 72234; Mature: 72103

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLIQLLLISLAFIMASVSHYAVSATTDLQRADELFDLLDSGVVEKNLEKKQYLEELTP
CHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCC
LVPKNDLARQLRLARTICWSYNMQDDAEIAQALLFAKQALTRPELTNFPDDKLDLELCYA
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
WFSEQKGDVDTALSYYNRLVIDAYKLENLRLMADVRMMRGYLTSFQGNYTQGLEDLMTAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLYKSLNVTVWVQHSLYEIATIYRRLGDQENAIRYFNQLHKSHIDNQEFDKANVVISSIA
HHHHHCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IAEEERKSFTQAKKLFLESYHYWNTNQNGFKKAGVAVNMAGTLIKLGEINQALKYLDESV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEECHHHHHHHHHHHHHHHHHHHCC
PFITPSAEGFYNYMQLFYAQIYLELGQFEQAHKSIALARAAFERVKSLRGLVQVQLVESQ
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCE
IFLMQDNWQAAYESLYKYMELHMELDKKLFSRYNTEMRTRFNAEQIEAENRHLVENHKLK
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHCCCCCHHHHCCCHH
ELELAMLEQNKVQQWIIIFMGSIILIIISTFTYKQSQKNKLLSALALTDDLTQLPNRRYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YTQAQDYFESAIKNQQALSCISFDADYFKLINDRYGHEVGDETLKLLADTCRAVLGTQYE
EEHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AARVGGEEFLILLPHTDKAQALKVAQHLVNQVRNADFSPFPEGFSITISAGVSSLSETDD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHH
KLSQLLKRADEALYLAKKQGRDQVKMN
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PKLIQLLLISLAFIMASVSHYAVSATTDLQRADELFDLLDSGVVEKNLEKKQYLEELTP
HHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCC
LVPKNDLARQLRLARTICWSYNMQDDAEIAQALLFAKQALTRPELTNFPDDKLDLELCYA
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
WFSEQKGDVDTALSYYNRLVIDAYKLENLRLMADVRMMRGYLTSFQGNYTQGLEDLMTAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLYKSLNVTVWVQHSLYEIATIYRRLGDQENAIRYFNQLHKSHIDNQEFDKANVVISSIA
HHHHHCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IAEEERKSFTQAKKLFLESYHYWNTNQNGFKKAGVAVNMAGTLIKLGEINQALKYLDESV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEECHHHHHHHHHHHHHHHHHHHCC
PFITPSAEGFYNYMQLFYAQIYLELGQFEQAHKSIALARAAFERVKSLRGLVQVQLVESQ
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCE
IFLMQDNWQAAYESLYKYMELHMELDKKLFSRYNTEMRTRFNAEQIEAENRHLVENHKLK
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHCCCCCHHHHCCCHH
ELELAMLEQNKVQQWIIIFMGSIILIIISTFTYKQSQKNKLLSALALTDDLTQLPNRRYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YTQAQDYFESAIKNQQALSCISFDADYFKLINDRYGHEVGDETLKLLADTCRAVLGTQYE
EEHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AARVGGEEFLILLPHTDKAQALKVAQHLVNQVRNADFSPFPEGFSITISAGVSSLSETDD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHH
KLSQLLKRADEALYLAKKQGRDQVKMN
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA