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The map label for this gene is pleD [H]

Identifier: 157963010

GI number: 157963010

Start: 3915263

End: 3917146

Strand: Direct

Name: pleD [H]

Synonym: Spea_3193

Alternate gene names: 157963010

Gene position: 3915263-3917146 (Clockwise)

Preceding gene: 157963009

Following gene: 157963011

Centisome position: 75.66

GC content: 43.63

Gene sequence:

>1884_bases
ATGCCTAAACTTTCCCTGTTAATACAAATTCTGCTATTTGTAAGCACTCTTACGGTGTGCAGTTTAGGCTTCGCCGAACA
AACTAACATCCAAAAGGCAGATAAAATTTTTGCCATTATCGATAGTGGCACCATTGACAATCCCGAAGCCTATAACCTGC
ATCTAACTCAATTAGAAAGCTTAATCTCAAAAGGGGATACCTCAAGACAACTGCGCCTACAACGAGCCCTGTGTTGGTCA
TTCAACCCTTATATAAAAGAGCAAATCCCAACAGCTTTAGCATTTGCCAAACACGTACTGACCAACCCTAAATTAGTAAA
CTTTCCAGACCATAAGCTCGACTTGGAGCTGTGCCACACTTGGTTTTCAGAGAAGCATGGCAACGTAGAAGCGGCGCTAA
AAGGTTATAACAAGATAATAGAAGAGGCCTATGCTCGAGAGAATTTACGCTTGGTGGCCGATGCCAGAAATATGCGTGGC
TTCTTACGATCTTACCAAGGTAACTTTACTCAAGGACTCGAAGACTTACTGATAGCCCATGCTTTGTATCAAAATTTAAA
CCTACCCGCTTGGGCTCATAGCAGCTTGTATGAAATCGCCACCAGTTATCGCCGCCTAGGCGAACATAAAAATGCTATTC
GTTACTTTAAACAAGTTCAAAACGGCCATATTAACAAGCAAAACTTTGATGGTGCCAATGCCATATCTGTCTCAATTGCC
ATTGCTGAAGAAGAACAAGGTAATCTCGAAGTCGCAAAGACATTGTTTGAAGAGAGTCATGACTATTGGAGAAACAACAA
AAAAGAGTTAGAGCAAGCTACTGTTGCAGTGAATATCGCGAGTACCTTGATAAAACTCGGTGAGTTTACTCAAGCTCACC
AATACCTAGATGAAGCCGAGCCGTTTATCCTGTCTACAGATACCGCGTTTTACAGCTTTTTACATCTATTTCGCGCACAA
GCGTACCTTGCAGAGAGTCAACTGTCTCAGGCTCACAAGAGTATTGCATACGCCCGCAGCGCTTTTAGCAGAGTGAAGAA
CATACGCGGGTTGGCACAATTGCAATTAGTCGAAAGCCAGGCATTTCTCAAGCAAAAAGATTGGAAACAAGCCTACTATG
CACTCTCTGAATATATGAAGTTGCATATCGAACTCGATAGTAAGCAACTTTCAAGCTACACCACAGAGATGCGTACCCGC
TTCAATGCCGATCAAATTGAACAAGAAAACCGTCATCTTATAGAAAATCAGCAGCTCAGAGAATTAGAGCTGGCCATGTT
AGAACAAAATGAACTACAGCAAGGGATCATTATCTTTCTAGGTGGCTTTATCATCTTGATTGTTTCGGTGTTTGCCTATA
AACAAATTCAAAAGAACAAACTATTATCGGCACTTGCGCTAACGGATGACCTCACCCAACTTCCCAACCGACGCTATATT
TATGCTAAGGCTCAGACCTACTTCGAACAGGCTCAAAGGACCGAGAGTCCATTATCGGTGATCACCTTCGATATCGACTA
TTTCAAGCTAATTAACGACAAGTTTGGTCACGAAGTCGGCGATCACGCCTTAAAGCTTCTGGCGGATACATGCCGCGAAG
TGCTCGCAACACAATATAAGACCGCCCGTATTGGCGGCGAAGAGTTTTTAGTTATCTTGCCTGATACCGATTTGGCACAG
GGTTATAAGATAGCTCAGGCCTTGGTGCATAGTGTCAGATACGGTGATTTTAGCCAACTACCATCAGGGTTTAGCATGAC
TATCAGTGCCGGTGTCGCAAGCCTCAGCACTGCGGACAGCAAGTTGTCGCTGCTCCTCAAAAAAGCCGACCAAGCCTTGT
ACATAGCTAAAACAGAAGGTCGCAACCAAGCTAGGATGGGTTAA

Upstream 100 bases:

>100_bases
CTCTATTACTCGGAACGACACTAAAGCTTGCTATACCTCATATTGCATAGTGTATAATTAGGCCTAAATATCAATAACTT
CCACTCGAAGTCGACTCTAA

Downstream 100 bases:

>100_bases
CTGTGCTACAGATGGGGATACCTATTCATCCCCAATAACTGCTATCATTAGCGGTTATTCTAAGCCGTTACTTTTCACCA
ATACTTTTAGCCAATACTTT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MPKLSLLIQILLFVSTLTVCSLGFAEQTNIQKADKIFAIIDSGTIDNPEAYNLHLTQLESLISKGDTSRQLRLQRALCWS
FNPYIKEQIPTALAFAKHVLTNPKLVNFPDHKLDLELCHTWFSEKHGNVEAALKGYNKIIEEAYARENLRLVADARNMRG
FLRSYQGNFTQGLEDLLIAHALYQNLNLPAWAHSSLYEIATSYRRLGEHKNAIRYFKQVQNGHINKQNFDGANAISVSIA
IAEEEQGNLEVAKTLFEESHDYWRNNKKELEQATVAVNIASTLIKLGEFTQAHQYLDEAEPFILSTDTAFYSFLHLFRAQ
AYLAESQLSQAHKSIAYARSAFSRVKNIRGLAQLQLVESQAFLKQKDWKQAYYALSEYMKLHIELDSKQLSSYTTEMRTR
FNADQIEQENRHLIENQQLRELELAMLEQNELQQGIIIFLGGFIILIVSVFAYKQIQKNKLLSALALTDDLTQLPNRRYI
YAKAQTYFEQAQRTESPLSVITFDIDYFKLINDKFGHEVGDHALKLLADTCREVLATQYKTARIGGEEFLVILPDTDLAQ
GYKIAQALVHSVRYGDFSQLPSGFSMTISAGVASLSTADSKLSLLLKKADQALYIAKTEGRNQARMG

Sequences:

>Translated_627_residues
MPKLSLLIQILLFVSTLTVCSLGFAEQTNIQKADKIFAIIDSGTIDNPEAYNLHLTQLESLISKGDTSRQLRLQRALCWS
FNPYIKEQIPTALAFAKHVLTNPKLVNFPDHKLDLELCHTWFSEKHGNVEAALKGYNKIIEEAYARENLRLVADARNMRG
FLRSYQGNFTQGLEDLLIAHALYQNLNLPAWAHSSLYEIATSYRRLGEHKNAIRYFKQVQNGHINKQNFDGANAISVSIA
IAEEEQGNLEVAKTLFEESHDYWRNNKKELEQATVAVNIASTLIKLGEFTQAHQYLDEAEPFILSTDTAFYSFLHLFRAQ
AYLAESQLSQAHKSIAYARSAFSRVKNIRGLAQLQLVESQAFLKQKDWKQAYYALSEYMKLHIELDSKQLSSYTTEMRTR
FNADQIEQENRHLIENQQLRELELAMLEQNELQQGIIIFLGGFIILIVSVFAYKQIQKNKLLSALALTDDLTQLPNRRYI
YAKAQTYFEQAQRTESPLSVITFDIDYFKLINDKFGHEVGDHALKLLADTCREVLATQYKTARIGGEEFLVILPDTDLAQ
GYKIAQALVHSVRYGDFSQLPSGFSMTISAGVASLSTADSKLSLLLKKADQALYIAKTEGRNQARMG
>Mature_626_residues
PKLSLLIQILLFVSTLTVCSLGFAEQTNIQKADKIFAIIDSGTIDNPEAYNLHLTQLESLISKGDTSRQLRLQRALCWSF
NPYIKEQIPTALAFAKHVLTNPKLVNFPDHKLDLELCHTWFSEKHGNVEAALKGYNKIIEEAYARENLRLVADARNMRGF
LRSYQGNFTQGLEDLLIAHALYQNLNLPAWAHSSLYEIATSYRRLGEHKNAIRYFKQVQNGHINKQNFDGANAISVSIAI
AEEEQGNLEVAKTLFEESHDYWRNNKKELEQATVAVNIASTLIKLGEFTQAHQYLDEAEPFILSTDTAFYSFLHLFRAQA
YLAESQLSQAHKSIAYARSAFSRVKNIRGLAQLQLVESQAFLKQKDWKQAYYALSEYMKLHIELDSKQLSSYTTEMRTRF
NADQIEQENRHLIENQQLRELELAMLEQNELQQGIIIFLGGFIILIVSVFAYKQIQKNKLLSALALTDDLTQLPNRRYIY
AKAQTYFEQAQRTESPLSVITFDIDYFKLINDKFGHEVGDHALKLLADTCREVLATQYKTARIGGEEFLVILPDTDLAQG
YKIAQALVHSVRYGDFSQLPSGFSMTISAGVASLSTADSKLSLLLKKADQALYIAKTEGRNQARMG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=189, Percent_Identity=34.3915343915344, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=34.6820809248555, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787262, Length=189, Percent_Identity=32.2751322751323, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=33.5227272727273, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=32.8042328042328, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=30.8176100628931, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=208, Percent_Identity=30.2884615384615, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=33.1578947368421, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=32.1212121212121, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=27.3224043715847, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=29.940119760479, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 71250; Mature: 71119

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLSLLIQILLFVSTLTVCSLGFAEQTNIQKADKIFAIIDSGTIDNPEAYNLHLTQLES
CCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHEEEEEECCCCCCCCCCEEEHHHHHH
LISKGDTSRQLRLQRALCWSFNPYIKEQIPTALAFAKHVLTNPKLVNFPDHKLDLELCHT
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH
WFSEKHGNVEAALKGYNKIIEEAYARENLRLVADARNMRGFLRSYQGNFTQGLEDLLIAH
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALYQNLNLPAWAHSSLYEIATSYRRLGEHKNAIRYFKQVQNGHINKQNFDGANAISVSIA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
IAEEEQGNLEVAKTLFEESHDYWRNNKKELEQATVAVNIASTLIKLGEFTQAHQYLDEAE
EECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFILSTDTAFYSFLHLFRAQAYLAESQLSQAHKSIAYARSAFSRVKNIRGLAQLQLVESQ
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLKQKDWKQAYYALSEYMKLHIELDSKQLSSYTTEMRTRFNADQIEQENRHLIENQQLR
HHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
ELELAMLEQNELQQGIIIFLGGFIILIVSVFAYKQIQKNKLLSALALTDDLTQLPNRRYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YAKAQTYFEQAQRTESPLSVITFDIDYFKLINDKFGHEVGDHALKLLADTCREVLATQYK
EEHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
TARIGGEEFLVILPDTDLAQGYKIAQALVHSVRYGDFSQLPSGFSMTISAGVASLSTADS
HHEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHHHHHCCHH
KLSLLLKKADQALYIAKTEGRNQARMG
HHHHHHHHCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PKLSLLIQILLFVSTLTVCSLGFAEQTNIQKADKIFAIIDSGTIDNPEAYNLHLTQLES
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHEEEEEECCCCCCCCCCEEEHHHHHH
LISKGDTSRQLRLQRALCWSFNPYIKEQIPTALAFAKHVLTNPKLVNFPDHKLDLELCHT
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHH
WFSEKHGNVEAALKGYNKIIEEAYARENLRLVADARNMRGFLRSYQGNFTQGLEDLLIAH
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALYQNLNLPAWAHSSLYEIATSYRRLGEHKNAIRYFKQVQNGHINKQNFDGANAISVSIA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
IAEEEQGNLEVAKTLFEESHDYWRNNKKELEQATVAVNIASTLIKLGEFTQAHQYLDEAE
EECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFILSTDTAFYSFLHLFRAQAYLAESQLSQAHKSIAYARSAFSRVKNIRGLAQLQLVESQ
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFLKQKDWKQAYYALSEYMKLHIELDSKQLSSYTTEMRTRFNADQIEQENRHLIENQQLR
HHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
ELELAMLEQNELQQGIIIFLGGFIILIVSVFAYKQIQKNKLLSALALTDDLTQLPNRRYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YAKAQTYFEQAQRTESPLSVITFDIDYFKLINDKFGHEVGDHALKLLADTCREVLATQYK
EEHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
TARIGGEEFLVILPDTDLAQGYKIAQALVHSVRYGDFSQLPSGFSMTISAGVASLSTADS
HHEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHHHHHCCHH
KLSLLLKKADQALYIAKTEGRNQARMG
HHHHHHHHCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA