Click here to switch to the map view.

The map label for this gene is nrfB [C]

Identifier: 157962520

GI number: 157962520

Start: 3296698

End: 3297684

Strand: Direct

Name: nrfB [C]

Synonym: Spea_2699

Alternate gene names: 157962520

Gene position: 3296698-3297684 (Clockwise)

Preceding gene: 157962519

Following gene: 157962521

Centisome position: 63.71

GC content: 49.04

Gene sequence:

>987_bases
ATGAAGATCACAAAAAGATTAAAAACCTTACTACCGGCCTTAATGGCGTCGGCGGTACTGTCTCTTGGTTTTGCTCAAAC
CGCCACTGCTTCTAAGTGGGATGCCAAGATGACTCCTGATGAAGTCGAAGCTACGTTAGATCAGAAATTTGCTGAAGGTA
AGTACTCACCAAAAGGTGCAGACTCTTGCCTTATGTGTCACCGCAAGTCTGAAAAAGTGATGGACCTGTTCAAAGGCGTT
CACGGCGCTGTTGACTCAAGCAAGAGCCCAATGGCTGGCCTTCAATGTGAAGCGTGTCACGGCCCTATGGGTAAACATAA
CCGCGGCGGTAACGAGCCGATGATCGCTTTTGGTCCAGACTCAACTCTGGCTCCAGAGCTGCAAAACAGTGTTTGTATGA
GCTGTCATAAAGACGACAAGCGTATGGATTGGAATGGTGGACACCATGACAATGCAGATGTGGCTTGTGCTTCTTGTCAC
TCTGTACACACAGCTAAAGATCCTGTGCTGTCTAAAAACACTGAAATGGAAGTTTGTACTAGCTGTCATACTCAGCAAAA
AGCTGACATGAACAAGCGTTCAAGCCACCCAATGAAGTGGGCGCAAATGGTGTGTAGCGATTGTCATAATCCACACGGCA
CCATGAGCGACGCCGATCTTGTTAAGCCTAGCGTTAACGAAACTTGCTATTCATGCCATGCGGAGAAGCGCGGCCCAAAA
CTGTGGGAGCATGCACCCGTTACTGAAGACTGCGTGACATGCCACAACCCACACGGCAGCGTTAACGAAGGCATGCTAAA
AACTCGTGCTCCACAGCTTTGTCAACAGTGTCACTCTTCTGCGCATAGCGGTCAGGCACTATTTGGCAACACTGAACAAG
GTTCTTCTGTTGGCGGCAATGCCATGACTGGCGGCCGTAGCTGTTTGAACTGTCACAACCAGATCCATGGTTCTAACCAT
CCATCTGGCAAGCTACTTCAGCGCTAA

Upstream 100 bases:

>100_bases
TGGACTCAAGTGTTAACCACCTGAGTCCAAGAGTGTTGAAAAAAGGGGGAGGCCTCTCCCCCACTTTCTCTTCAAAATTG
TGCAAGTTTAGGAAGCTATT

Downstream 100 bases:

>100_bases
GGAGACGAGAAGATGAAATTCAAACTAAATTTGGTCACGCTCGCTTTGCTAACTAACGCCGGAATGTTATCTGGCGCGGC
TATCGCTGCTGACGGTTACG

Product: cytochrome C family protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Decaheme C-Type Cytochrome DmsE Family; Decaheme Cytochrome C MtrA; Multiheme Cytochrome; Decaheme Cytochrome C; Nitrate/TMAO Reductase; Doubled CXXCH Domain-Containing Protein; Multiheme C-Type Cytochrome; Cytochrome; Lipoprotein Cytochrome C; Decaheme Cytochrome C MtrD; Multiheme Cytochrome Family Protein; Double CXXCH Motif-Containing Protein; Cytochrome C; Cytochrome C-Type Protein NrfB; Cytochrome C Nitrite Reductase Pentaheme Subunit

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKITKRLKTLLPALMASAVLSLGFAQTATASKWDAKMTPDEVEATLDQKFAEGKYSPKGADSCLMCHRKSEKVMDLFKGV
HGAVDSSKSPMAGLQCEACHGPMGKHNRGGNEPMIAFGPDSTLAPELQNSVCMSCHKDDKRMDWNGGHHDNADVACASCH
SVHTAKDPVLSKNTEMEVCTSCHTQQKADMNKRSSHPMKWAQMVCSDCHNPHGTMSDADLVKPSVNETCYSCHAEKRGPK
LWEHAPVTEDCVTCHNPHGSVNEGMLKTRAPQLCQQCHSSAHSGQALFGNTEQGSSVGGNAMTGGRSCLNCHNQIHGSNH
PSGKLLQR

Sequences:

>Translated_328_residues
MKITKRLKTLLPALMASAVLSLGFAQTATASKWDAKMTPDEVEATLDQKFAEGKYSPKGADSCLMCHRKSEKVMDLFKGV
HGAVDSSKSPMAGLQCEACHGPMGKHNRGGNEPMIAFGPDSTLAPELQNSVCMSCHKDDKRMDWNGGHHDNADVACASCH
SVHTAKDPVLSKNTEMEVCTSCHTQQKADMNKRSSHPMKWAQMVCSDCHNPHGTMSDADLVKPSVNETCYSCHAEKRGPK
LWEHAPVTEDCVTCHNPHGSVNEGMLKTRAPQLCQQCHSSAHSGQALFGNTEQGSSVGGNAMTGGRSCLNCHNQIHGSNH
PSGKLLQR
>Mature_328_residues
MKITKRLKTLLPALMASAVLSLGFAQTATASKWDAKMTPDEVEATLDQKFAEGKYSPKGADSCLMCHRKSEKVMDLFKGV
HGAVDSSKSPMAGLQCEACHGPMGKHNRGGNEPMIAFGPDSTLAPELQNSVCMSCHKDDKRMDWNGGHHDNADVACASCH
SVHTAKDPVLSKNTEMEVCTSCHTQQKADMNKRSSHPMKWAQMVCSDCHNPHGTMSDADLVKPSVNETCYSCHAEKRGPK
LWEHAPVTEDCVTCHNPHGSVNEGMLKTRAPQLCQQCHSSAHSGQALFGNTEQGSSVGGNAMTGGRSCLNCHNQIHGSNH
PSGKLLQR

Specific function: Plays A Role In Nitrite Reduction. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=199, Percent_Identity=33.1658291457286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35418; Mature: 35418

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
11.3 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
11.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKRLKTLLPALMASAVLSLGFAQTATASKWDAKMTPDEVEATLDQKFAEGKYSPKGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DSCLMCHRKSEKVMDLFKGVHGAVDSSKSPMAGLQCEACHGPMGKHNRGGNEPMIAFGPD
CHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCCCCCCCCCCCEEEECCC
STLAPELQNSVCMSCHKDDKRMDWNGGHHDNADVACASCHSVHTAKDPVLSKNTEMEVCT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
SCHTQQKADMNKRSSHPMKWAQMVCSDCHNPHGTMSDADLVKPSVNETCYSCHAEKRGPK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCC
LWEHAPVTEDCVTCHNPHGSVNEGMLKTRAPQLCQQCHSSAHSGQALFGNTEQGSSVGGN
CHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
AMTGGRSCLNCHNQIHGSNHPSGKLLQR
CCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKRLKTLLPALMASAVLSLGFAQTATASKWDAKMTPDEVEATLDQKFAEGKYSPKGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DSCLMCHRKSEKVMDLFKGVHGAVDSSKSPMAGLQCEACHGPMGKHNRGGNEPMIAFGPD
CHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCCCCCCCCCCCEEEECCC
STLAPELQNSVCMSCHKDDKRMDWNGGHHDNADVACASCHSVHTAKDPVLSKNTEMEVCT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHH
SCHTQQKADMNKRSSHPMKWAQMVCSDCHNPHGTMSDADLVKPSVNETCYSCHAEKRGPK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCC
LWEHAPVTEDCVTCHNPHGSVNEGMLKTRAPQLCQQCHSSAHSGQALFGNTEQGSSVGGN
CHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
AMTGGRSCLNCHNQIHGSNHPSGKLLQR
CCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA