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The map label for this gene is tig [H]

Identifier: 157962503

GI number: 157962503

Start: 3268300

End: 3269604

Strand: Reverse

Name: tig [H]

Synonym: Spea_2682

Alternate gene names: 157962503

Gene position: 3269604-3268300 (Counterclockwise)

Preceding gene: 157962505

Following gene: 157962502

Centisome position: 63.19

GC content: 45.13

Gene sequence:

>1305_bases
ATGCAAGTTTCTGTTGAAACCACACAAGGCCTAGAGCGTCGCCTAACAATTTCTGTACCTGCTGAGCAGATCGAGAACAC
TGTTAAAGAAGCATTAAAAAGCGAAGCTAAGCGTGCCCGTATCCCAGGTTTCCGTCCAGGAAAAGTACCTGTAAGCGTAA
TTAATAAGCGTTATGGTAGTGCAATTCGTCAAGATATCATGGGTGAAGTGATGCAGCGTAACTTCGTTGAAGCAATCATT
GCTGAGAAGTTAAACCCAGCTGGCGCACCTACATTAACTCCTGGTTCAACTGAAGGCGAAAACTTTGAGTTTGTTGCTAC
TTTTGAAATCTACCCAGAAGTAGAGCTAAAAGGTCTTGAGTCAATCACTGTTGAACAGCCTACTGCTGAAGTAACAGAAG
CTGACGTTGACGCTATGATTGAAACGCTACGTAACCAACACGCTACTTTCGAAGTAGCAGACCGCGCAGCGGCTGAAGGC
GACAAAGCTAAAATCAACTTCGTAGGTTCAATCGATGGCGAAGAATTCGAAGGCGGTAAAGCTGACGATTTCGAACTACA
ACTAGGCAGCGGCCGTATGATCCCAGGCTTCGAGTCTGGCGTTGAAGGTCACAAAGCTGGTGAAGAGTTCAACATCGAAG
TGACTTTCCCAGAAGATTACCACGCTGAAAACCTAAAGGGTAAAGTAGCGACTTTCGCTATCACTCTGAACGAAGTTCAA
GCGGCTAACCTTCCAGAAGTTAACGACGAGTTCGCTACACTATTCGGTATTACTGAAGGTGGCATCGACGCACTTCGCGC
TGAAATCAGCAAGAACATGAGCCGTGAACTTGAGCAAGCGCTTAAAGCTAACGTTAAAGAGCAAGTTCTTAATGGTCTAG
TTGAGCAAAACGACATCGAATTGCCAACAGCACTTATCAACGGTGAAGTAGAAGTATTGCGTAAGCAAGCTATGCAACGT
TTCGGCGACCAAGCTGCAAACATGCCTGAGCTTCCAGCTGACCTGTTCACTGAACAAGCTGCACGTCGCGTTAAAGTAGG
TCTACTACTTGGCGAAGTGATCAAGACTAACGAGCTAAAAGCTGAAGATGAACGCGTTCAAGGTTTGATCGCTTCAATGG
CTTCTGCTTACGAAGATCCAAGTGAAGTTGTAGCTTACTACAATGGTAACGAAGAACTTATGCAAAACATGCGCAACGTT
GCTCTAGAAGAGCAAGCAGTTGAAGCTTTGTTAAAAACTGCAACTTTGACTGAAAAAGCAGTCAACTTTGAAGAATTTAT
GAACAAGGCTACAGGTCGCGCGTAA

Upstream 100 bases:

>100_bases
ATGGGCATTTTGGTGAAATTTACGGATCAAGAAACGAATACCTGAGTAGTTGATTTTTCGACTATTGCAAAGGACGATAG
ACATATTTCGAGGTAAAAAA

Downstream 100 bases:

>100_bases
CCATCGCTTGACTTAAATGGCTATTAGCCATTTATAATGGCTCGTATGAGGTTCCTCATGCGGGCCATTTTTATTTAGGG
AAGTTAATCATGCACAAAGC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGSAIRQDIMGEVMQRNFVEAII
AEKLNPAGAPTLTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDAMIETLRNQHATFEVADRAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRAEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPTALINGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKTNELKAEDERVQGLIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA

Sequences:

>Translated_434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGSAIRQDIMGEVMQRNFVEAII
AEKLNPAGAPTLTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDAMIETLRNQHATFEVADRAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRAEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPTALINGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKTNELKAEDERVQGLIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA
>Mature_434_residues
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGSAIRQDIMGEVMQRNFVEAII
AEKLNPAGAPTLTPGSTEGENFEFVATFEIYPEVELKGLESITVEQPTAEVTEADVDAMIETLRNQHATFEVADRAAAEG
DKAKINFVGSIDGEEFEGGKADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
AANLPEVNDEFATLFGITEGGIDALRAEISKNMSRELEQALKANVKEQVLNGLVEQNDIELPTALINGEVEVLRKQAMQR
FGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKTNELKAEDERVQGLIASMASAYEDPSEVVAYYNGNEELMQNMRNV
ALEEQAVEALLKTATLTEKAVNFEEFMNKATGRA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=430, Percent_Identity=60.4651162790698, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 47543; Mature: 47543

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGS
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AIRQDIMGEVMQRNFVEAIIAEKLNPAGAPTLTPGSTEGENFEFVATFEIYPEVELKGLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
SITVEQPTAEVTEADVDAMIETLRNQHATFEVADRAAAEGDKAKINFVGSIDGEEFEGGK
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCCCC
ADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
CCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHC
AANLPEVNDEFATLFGITEGGIDALRAEISKNMSRELEQALKANVKEQVLNGLVEQNDIE
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LPTALINGEVEVLRKQAMQRFGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKTNELK
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEDERVQGLIASMASAYEDPSEVVAYYNGNEELMQNMRNVALEEQAVEALLKTATLTEKA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNFEEFMNKATGRA
CCHHHHHHHHCCCC
>Mature Secondary Structure
MQVSVETTQGLERRLTISVPAEQIENTVKEALKSEAKRARIPGFRPGKVPVSVINKRYGS
CEEECCHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AIRQDIMGEVMQRNFVEAIIAEKLNPAGAPTLTPGSTEGENFEFVATFEIYPEVELKGLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEECCCC
SITVEQPTAEVTEADVDAMIETLRNQHATFEVADRAAAEGDKAKINFVGSIDGEEFEGGK
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCCCC
ADDFELQLGSGRMIPGFESGVEGHKAGEEFNIEVTFPEDYHAENLKGKVATFAITLNEVQ
CCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHC
AANLPEVNDEFATLFGITEGGIDALRAEISKNMSRELEQALKANVKEQVLNGLVEQNDIE
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LPTALINGEVEVLRKQAMQRFGDQAANMPELPADLFTEQAARRVKVGLLLGEVIKTNELK
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AEDERVQGLIASMASAYEDPSEVVAYYNGNEELMQNMRNVALEEQAVEALLKTATLTEKA
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNFEEFMNKATGRA
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA