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The map label for this gene is purN [H]

Identifier: 157962399

GI number: 157962399

Start: 3137475

End: 3138119

Strand: Direct

Name: purN [H]

Synonym: Spea_2578

Alternate gene names: 157962399

Gene position: 3137475-3138119 (Clockwise)

Preceding gene: 157962398

Following gene: 157962402

Centisome position: 60.63

GC content: 47.6

Gene sequence:

>645_bases
ATGCCCCAGAGTTGTCGCGTTTTAGTATTAATTTCAGGTAACGGCAGTAACCTACAAGCCATTATCGATGGTTGTGACGA
TAACCTCCAAGCCGAAGTTGTTGGCGTGATCAGTAATAAGCCCGATGCCTATGGATTGGTTCGTGCACACCAAAATGAGA
TTGATACCAGCTGCGTGATAGCTCACACAGGCGAAACAAGACAAGAGTACGACGCTAGACTACTCAATGCGATAGAAAAG
TATCAGCCAGATCTTGTGGTATTAGCTGGCTTTATGCGCATTCTAAGCGATGAGTTCGTACAGCGTTTTGAAGGTAAGAT
GCTCAACATCCACCCTTCTCTCTTGCCTAAATATACAGGTTTACACACCCATCAACGTGCCATTGACGCTAATGACACCG
AGCACGGCGCTAGCGTACATTTTGTGACGCCAGAACTCGATGCAGGTCCGGTGATCTTACAGGCGAAAGTGCCAGTTTAT
GCAGATGATACTGCCGACACATTGGCAGAGCGCGTTCATGAACAGGAACATGCTATTTACCCACTCGTGGTTAAATGGTT
CAGCCAACAACGTCTAGCAATGGTAGACGGTATTGCCATGCTTGATGGTCAAGCGCTTCCAGCTGACGGCTACGCTGCTG
ACTAA

Upstream 100 bases:

>100_bases
ATTCGGCCTTAGAACTGCTAACAGCAGAAGGCGAGAACGCTTGGTTAATCGGTAACATTGCCAAGCGTAACGGCGAAGAA
GAGCAAGTGGAGATCCTGTA

Downstream 100 bases:

>100_bases
ACAAATGTTTAGATAGCAAAAATACAAAGGCACCTGACGGTGCCTTTTTTATTCCATTTATAAAACCTTGCTAATACCAA
TCAGTATAAACATTTAGTCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEK
YQPDLVVLAGFMRILSDEFVQRFEGKMLNIHPSLLPKYTGLHTHQRAIDANDTEHGASVHFVTPELDAGPVILQAKVPVY
ADDTADTLAERVHEQEHAIYPLVVKWFSQQRLAMVDGIAMLDGQALPADGYAAD

Sequences:

>Translated_214_residues
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEK
YQPDLVVLAGFMRILSDEFVQRFEGKMLNIHPSLLPKYTGLHTHQRAIDANDTEHGASVHFVTPELDAGPVILQAKVPVY
ADDTADTLAERVHEQEHAIYPLVVKWFSQQRLAMVDGIAMLDGQALPADGYAAD
>Mature_213_residues
PQSCRVLVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHQNEIDTSCVIAHTGETRQEYDARLLNAIEKY
QPDLVVLAGFMRILSDEFVQRFEGKMLNIHPSLLPKYTGLHTHQRAIDANDTEHGASVHFVTPELDAGPVILQAKVPVYA
DDTADTLAERVHEQEHAIYPLVVKWFSQQRLAMVDGIAMLDGQALPADGYAAD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=47.7777777777778, Blast_Score=161, Evalue=3e-40,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=47.7777777777778, Blast_Score=161, Evalue=3e-40,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=47.7777777777778, Blast_Score=161, Evalue=3e-40,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=60.7655502392344, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1787483, Length=164, Percent_Identity=34.1463414634146, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=38.4180790960452, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=31.9796954314721, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24582400, Length=202, Percent_Identity=47.5247524752475, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23486; Mature: 23355

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSCRVLVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHQNEIDTSCVI
CCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEEE
AHTGETRQEYDARLLNAIEKYQPDLVVLAGFMRILSDEFVQRFEGKMLNIHPSLLPKYTG
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCC
LHTHQRAIDANDTEHGASVHFVTPELDAGPVILQAKVPVYADDTADTLAERVHEQEHAIY
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHHCCHH
PLVVKWFSQQRLAMVDGIAMLDGQALPADGYAAD
HHHHHHHCCCHHHHHCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
PQSCRVLVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHQNEIDTSCVI
CCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEEE
AHTGETRQEYDARLLNAIEKYQPDLVVLAGFMRILSDEFVQRFEGKMLNIHPSLLPKYTG
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCC
LHTHQRAIDANDTEHGASVHFVTPELDAGPVILQAKVPVYADDTADTLAERVHEQEHAIY
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHHCCHH
PLVVKWFSQQRLAMVDGIAMLDGQALPADGYAAD
HHHHHHHCCCHHHHHCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]