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The map label for this gene is garR [C]

Identifier: 157962310

GI number: 157962310

Start: 3035681

End: 3036556

Strand: Reverse

Name: garR [C]

Synonym: Spea_2489

Alternate gene names: 157962310

Gene position: 3036556-3035681 (Counterclockwise)

Preceding gene: 157962311

Following gene: 157962307

Centisome position: 58.68

GC content: 48.4

Gene sequence:

>876_bases
ATGGCTAAAGTTGCATTTATTGGTTTGGGCGTGATGGGTTACCCCATGGCCGGGCATCTTGTTAAACATGGGCATCAAGT
CACGGTTTATAACCGCACAGAGGCGAAAGCTCAAGCTTGGGCAAAAGAGCATGGCGGCGAATATCAACTCACTCCAAGAG
CGGCGGCCGAAGGACAAGAGATTGTTTTTGTATGTGTCGGAAATGATGACGATTTACGTCAGGTAACCTTAGGCGATACC
GGTGCTTTGGCTGGTATGGACTCTGAAAGTATTTTAGTTGATCACACTACAGCTTCTGCCGATGTTGCCCGAGAAATTAA
CGCGGTAGCGCTGTCATTAGATATTGGTTTTATGGACGCTCCTGTATCAGGCGGACAAGCTGGCGCTGAAAACGGTGTCT
TGACTGTGATGGCTGGCGGCGAACAATCAACATTCGATACGGCAAAGCCTGTCATTGACAGTTATGCTCGCTGCGTAGAG
TTGCTAGGTGAGGTTGGAGCTGGACAACTGACTAAGATGGTTAACCAAATTTGTATCGCTGGTGTGGTCCAAGGTCTAGC
TGAAGGCCTGCATTTTGCTCGAAGCGCAGGCTTAGATGGTGAAAAGGTCGTCGAAGTGATCAGTAAAGGAGCGGCACAAT
CCTGGCAGATGGAAAACCGTTATCAAACCATGTGGCAGGGCGAGTATAACCTTGGTTTTGCAGTTGATTGGATGCGTAAA
GACTTAGGAATTGCGCTCACTGAAGCGCGCCGCAACGGCTCTCACTTGCCGTTAACGGCTTTAGTCGATCAATTTTATTC
AGAAGTGCAGGGTCTTGGTGGTTCTCGTTGGGATACATCGAGTCTTTTAGCAAAACTGGAAAAGAATCGTGGTTAG

Upstream 100 bases:

>100_bases
GGCTGGCTTTTTTATTGCCTGTTTTTTCTGTTTTATTTATGGACTAGCCTTTAGAATGAGTAAAAATTCTAATTACCTCA
CTCTACAAGTAGGAAGTAAA

Downstream 100 bases:

>100_bases
GCTTTTAGTTTAACCCAACAAAAAGGACCACATTAGTGGTCCTTTTTCGTCTAGACTATTTCTAAAAATAGTCAGCTTTT
ACTCTGGCTCAACCACAATA

Product: NAD-binding 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFIGLGVMGYPMAGHLVKHGHQVTVYNRTEAKAQAWAKEHGGEYQLTPRAAAEGQEIVFVCVGNDDDLRQVTLGDT
GALAGMDSESILVDHTTASADVAREINAVALSLDIGFMDAPVSGGQAGAENGVLTVMAGGEQSTFDTAKPVIDSYARCVE
LLGEVGAGQLTKMVNQICIAGVVQGLAEGLHFARSAGLDGEKVVEVISKGAAQSWQMENRYQTMWQGEYNLGFAVDWMRK
DLGIALTEARRNGSHLPLTALVDQFYSEVQGLGGSRWDTSSLLAKLEKNRG

Sequences:

>Translated_291_residues
MAKVAFIGLGVMGYPMAGHLVKHGHQVTVYNRTEAKAQAWAKEHGGEYQLTPRAAAEGQEIVFVCVGNDDDLRQVTLGDT
GALAGMDSESILVDHTTASADVAREINAVALSLDIGFMDAPVSGGQAGAENGVLTVMAGGEQSTFDTAKPVIDSYARCVE
LLGEVGAGQLTKMVNQICIAGVVQGLAEGLHFARSAGLDGEKVVEVISKGAAQSWQMENRYQTMWQGEYNLGFAVDWMRK
DLGIALTEARRNGSHLPLTALVDQFYSEVQGLGGSRWDTSSLLAKLEKNRG
>Mature_290_residues
AKVAFIGLGVMGYPMAGHLVKHGHQVTVYNRTEAKAQAWAKEHGGEYQLTPRAAAEGQEIVFVCVGNDDDLRQVTLGDTG
ALAGMDSESILVDHTTASADVAREINAVALSLDIGFMDAPVSGGQAGAENGVLTVMAGGEQSTFDTAKPVIDSYARCVEL
LGEVGAGQLTKMVNQICIAGVVQGLAEGLHFARSAGLDGEKVVEVISKGAAQSWQMENRYQTMWQGEYNLGFAVDWMRKD
LGIALTEARRNGSHLPLTALVDQFYSEVQGLGGSRWDTSSLLAKLEKNRG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=27.8571428571429, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=29.4520547945205, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI145693186, Length=295, Percent_Identity=33.8983050847458, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=32.5259515570934, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790315, Length=296, Percent_Identity=28.7162162162162, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=32.9824561403509, Blast_Score=103, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=27.3722627737226, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=25.2669039145907, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=27.9461279461279, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=27.9461279461279, Blast_Score=120, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30959; Mature: 30828

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGYPMAGHLVKHGHQVTVYNRTEAKAQAWAKEHGGEYQLTPRAAAEGQE
CCEEEEEECCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEECCHHHCCCCE
IVFVCVGNDDDLRQVTLGDTGALAGMDSESILVDHTTASADVAREINAVALSLDIGFMDA
EEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHCEEEEEEECCEEEC
PVSGGQAGAENGVLTVMAGGEQSTFDTAKPVIDSYARCVELLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GVVQGLAEGLHFARSAGLDGEKVVEVISKGAAQSWQMENRYQTMWQGEYNLGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
DLGIALTEARRNGSHLPLTALVDQFYSEVQGLGGSRWDTSSLLAKLEKNRG
HCCEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVAFIGLGVMGYPMAGHLVKHGHQVTVYNRTEAKAQAWAKEHGGEYQLTPRAAAEGQE
CEEEEEECCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEECCHHHCCCCE
IVFVCVGNDDDLRQVTLGDTGALAGMDSESILVDHTTASADVAREINAVALSLDIGFMDA
EEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHCEEEEEEECCEEEC
PVSGGQAGAENGVLTVMAGGEQSTFDTAKPVIDSYARCVELLGEVGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GVVQGLAEGLHFARSAGLDGEKVVEVISKGAAQSWQMENRYQTMWQGEYNLGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
DLGIALTEARRNGSHLPLTALVDQFYSEVQGLGGSRWDTSSLLAKLEKNRG
HCCEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA