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The map label for this gene is insF1 [C]

Identifier: 157962287

GI number: 157962287

Start: 3008771

End: 3009523

Strand: Direct

Name: insF1 [C]

Synonym: Spea_2466

Alternate gene names: 157962287

Gene position: 3008771-3009523 (Clockwise)

Preceding gene: 157962286

Following gene: 157962292

Centisome position: 58.15

GC content: 39.71

Gene sequence:

>753_bases
ATGGAGTTAGCCCCTGTAAGAGCGATGATACTAGATATCCGTCGGTTTATGCCTCGGATTGGTGGCAAGAAACTCTACTT
TTTACTTAAACCTAAGTTCATCGAGAAAGGGATTAAACTTGGGCGCGATAACTTCTTTAGTTACTTGAAAAGTGAAGGCT
TGTTAGTCAAACCTAAGCGTAATTATACCAAGACGACTAACAGTAAACATTGGATGAAGAAACATCCAAATTTACTGAAA
GAGTTAGTGCCAACAGCACCTGAAGAGGTGTTTGTTAGTGATATAACATATGTGCAATCAGAGCAAGGCATACATTATCT
ATCATTGGTAACTGACGCGTTCAGTCGTAAAATAATGGGATATGAATTAAGTAATGAAATGAAAGCTACAGACGTAGTCA
AAGCGTTAGAAATGACGATAAGTAATCGGCAGTATCAACATCGAGCAGTGCATCACTCAGACAGAGGGTTACAGTATTGC
TCTGCCGTTTATCAGTTAGCGTTGCAAAGAAGTGACATCCGCGCATCAATGACCGATGGATATGACTGCTACCAAAACGC
ACTAGCAGAACGTATAAATGGGATATTAAAGCAAGAGTTTCTATTATCTCCTTGCTGCAACCTTAATGAGTTAAAGCAAC
TCGTTGAGGAGTCAATTTTTATATACAATGAGCTGAGACCGCACTTAAGCTTGGGTATGAAAACACCTAATCAAGTGCAT
AAAAAAGACCAGCAGCAGAAGCTACTGGTCTAG

Upstream 100 bases:

>100_bases
TCAAAAACAGAAAGTAACGAGCTTAAATCGCGCTTGTAAGCTTCTGGGTATAACAAGACAAGCTATCTATCAAAGAGAAC
GAAGAGCGAATTGTAGAGCA

Downstream 100 bases:

>100_bases
TAAAAACCGTCAACCTATTTCAGGACGGGACATCAATATGTACTGCCCTAAAACCCAAAACCAGACAAGTTATCGGCTGC
AACAATTTATTGAAAAGACT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MELAPVRAMILDIRRFMPRIGGKKLYFLLKPKFIEKGIKLGRDNFFSYLKSEGLLVKPKRNYTKTTNSKHWMKKHPNLLK
ELVPTAPEEVFVSDITYVQSEQGIHYLSLVTDAFSRKIMGYELSNEMKATDVVKALEMTISNRQYQHRAVHHSDRGLQYC
SAVYQLALQRSDIRASMTDGYDCYQNALAERINGILKQEFLLSPCCNLNELKQLVEESIFIYNELRPHLSLGMKTPNQVH
KKDQQQKLLV

Sequences:

>Translated_250_residues
MELAPVRAMILDIRRFMPRIGGKKLYFLLKPKFIEKGIKLGRDNFFSYLKSEGLLVKPKRNYTKTTNSKHWMKKHPNLLK
ELVPTAPEEVFVSDITYVQSEQGIHYLSLVTDAFSRKIMGYELSNEMKATDVVKALEMTISNRQYQHRAVHHSDRGLQYC
SAVYQLALQRSDIRASMTDGYDCYQNALAERINGILKQEFLLSPCCNLNELKQLVEESIFIYNELRPHLSLGMKTPNQVH
KKDQQQKLLV
>Mature_250_residues
MELAPVRAMILDIRRFMPRIGGKKLYFLLKPKFIEKGIKLGRDNFFSYLKSEGLLVKPKRNYTKTTNSKHWMKKHPNLLK
ELVPTAPEEVFVSDITYVQSEQGIHYLSLVTDAFSRKIMGYELSNEMKATDVVKALEMTISNRQYQHRAVHHSDRGLQYC
SAVYQLALQRSDIRASMTDGYDCYQNALAERINGILKQEFLLSPCCNLNELKQLVEESIFIYNELRPHLSLGMKTPNQVH
KKDQQQKLLV

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=210, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788405, Length=203, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787263, Length=203, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786753, Length=203, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786570, Length=203, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786490, Length=203, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789981, Length=163, Percent_Identity=34.9693251533742, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29028; Mature: 29028

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAPVRAMILDIRRFMPRIGGKKLYFLLKPKFIEKGIKLGRDNFFSYLKSEGLLVKPKR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHCCCEEECCCC
NYTKTTNSKHWMKKHPNLLKELVPTAPEEVFVSDITYVQSEQGIHYLSLVTDAFSRKIMG
CCCCCCCCHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
YELSNEMKATDVVKALEMTISNRQYQHRAVHHSDRGLQYCSAVYQLALQRSDIRASMTDG
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
YDCYQNALAERINGILKQEFLLSPCCNLNELKQLVEESIFIYNELRPHLSLGMKTPNQVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHH
KKDQQQKLLV
HHHHHHCCCC
>Mature Secondary Structure
MELAPVRAMILDIRRFMPRIGGKKLYFLLKPKFIEKGIKLGRDNFFSYLKSEGLLVKPKR
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHCCCEEECCCC
NYTKTTNSKHWMKKHPNLLKELVPTAPEEVFVSDITYVQSEQGIHYLSLVTDAFSRKIMG
CCCCCCCCHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
YELSNEMKATDVVKALEMTISNRQYQHRAVHHSDRGLQYCSAVYQLALQRSDIRASMTDG
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
YDCYQNALAERINGILKQEFLLSPCCNLNELKQLVEESIFIYNELRPHLSLGMKTPNQVH
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHH
KKDQQQKLLV
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]