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The map label for this gene is ddl

Identifier: 157962243

GI number: 157962243

Start: 2960862

End: 2961866

Strand: Direct

Name: ddl

Synonym: Spea_2422

Alternate gene names: 157962243

Gene position: 2960862-2961866 (Clockwise)

Preceding gene: 157962242

Following gene: 157962257

Centisome position: 57.22

GC content: 44.08

Gene sequence:

>1005_bases
ATGACCCAAAAGAATTTATTATTGTTGTGTGGTGGTGGCGGTGATGAGCACAGTATCTCACTCTTATCGGCCAACTTTTT
TGAAGCGCAACTCGCCACTTTGCCACACTTTAATGTGCTGCGCGTTGAACTCAATGCTAAAGGTCAATATCACACCCAAG
CGGGCGAGCTTTGCGAGCTAACCAATAGCCAGCAGCTCCGCTTTGATGATGAGACCAAAGCCCCATGGAATGTGGACTAT
GTGATCCCTTGTATTCACGGCTACCCAGGTGAGACAGGTGACATCCAGTCCTATTTTGAATTGATTAACCTACCCTATTT
TGGCTGTGACTCAGAATCAAGCAGCAACTGCTTCAATAAAGTCACCGCTAAGATGTGGTTTAGCGCTCTTGGGATCCCCA
ATACGCCTTATATTTTCTTAAGCGAATTTAATCAGCAATCTTTGGAACAAGCCGAGGCTGCATTGGCAAAATGGGGGTCT
ATCTTTATTAAAGCCGCCTCACAGGGCTCATCTGTCGGTTGTTACCGCGTCGATAGCATAGAGCAGCTAGCAAGCTCATT
AGAGGAGGCATTTACCTTCTCGCCATATGTGGTGATCGAGAAGACAATTACCGCCCGTGAATTAGAGGTAGCCGCCTATG
AAGCCGATGGTGAAATCATCGCGACCAAACCGGGTGAAATTATCTGCGCTAGCAATACTTTCTATAGCTTCGATGAGAAG
TATGCAGAAAACAGCCAAGCACAGACTGTGATTGAAGCTGATGTTGATGAGGCAATTGCCAAGCAAATTCAAGAATATGC
GATTAAAGCGTTTAAGGGGATGAAGTTACGTCACCTATCGAGAATCGACTTCTTCTTAACCGAAGACAATGAAATTTTGC
TTAATGAGATCAACACATTCCCAGGTCAAACGCAGATATCTATGTTCCCTAAGATGCTACAAAATCATGGACATAATTTT
GCTCAGTATCTTTCAGGTAATATTTTGGCGCAGTTAAAAAGTTAA

Upstream 100 bases:

>100_bases
CAGCATTTAAGCTAAATCGGCTAGGCAATACTTGCATTAAAAAGTGATTACGCCATAGTGATCCGGCTGACTTTTATCAT
GGGTAACAGGTGGAGACAAA

Downstream 100 bases:

>100_bases
TGTAAAAAGCCTAGATATAAAGCTTAGAGTAAAGACTAACGTCTTTAGCCTTGTCCCGTCCTGAAATAGGTTGACGGTTT
TTACTAGACCAGTAGCTTCT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTQKNLLLLCGGGGDEHSISLLSANFFEAQLATLPHFNVLRVELNAKGQYHTQAGELCELTNSQQLRFDDETKAPWNVDY
VIPCIHGYPGETGDIQSYFELINLPYFGCDSESSSNCFNKVTAKMWFSALGIPNTPYIFLSEFNQQSLEQAEAALAKWGS
IFIKAASQGSSVGCYRVDSIEQLASSLEEAFTFSPYVVIEKTITARELEVAAYEADGEIIATKPGEIICASNTFYSFDEK
YAENSQAQTVIEADVDEAIAKQIQEYAIKAFKGMKLRHLSRIDFFLTEDNEILLNEINTFPGQTQISMFPKMLQNHGHNF
AQYLSGNILAQLKS

Sequences:

>Translated_334_residues
MTQKNLLLLCGGGGDEHSISLLSANFFEAQLATLPHFNVLRVELNAKGQYHTQAGELCELTNSQQLRFDDETKAPWNVDY
VIPCIHGYPGETGDIQSYFELINLPYFGCDSESSSNCFNKVTAKMWFSALGIPNTPYIFLSEFNQQSLEQAEAALAKWGS
IFIKAASQGSSVGCYRVDSIEQLASSLEEAFTFSPYVVIEKTITARELEVAAYEADGEIIATKPGEIICASNTFYSFDEK
YAENSQAQTVIEADVDEAIAKQIQEYAIKAFKGMKLRHLSRIDFFLTEDNEILLNEINTFPGQTQISMFPKMLQNHGHNF
AQYLSGNILAQLKS
>Mature_333_residues
TQKNLLLLCGGGGDEHSISLLSANFFEAQLATLPHFNVLRVELNAKGQYHTQAGELCELTNSQQLRFDDETKAPWNVDYV
IPCIHGYPGETGDIQSYFELINLPYFGCDSESSSNCFNKVTAKMWFSALGIPNTPYIFLSEFNQQSLEQAEAALAKWGSI
FIKAASQGSSVGCYRVDSIEQLASSLEEAFTFSPYVVIEKTITARELEVAAYEADGEIIATKPGEIICASNTFYSFDEKY
AENSQAQTVIEADVDEAIAKQIQEYAIKAFKGMKLRHLSRIDFFLTEDNEILLNEINTFPGQTQISMFPKMLQNHGHNFA
QYLSGNILAQLKS

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=31.3218390804598, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1786280, Length=247, Percent_Identity=30.3643724696356, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SHEPA (A8H5A5)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502277.1
- ProteinModelPortal:   A8H5A5
- SMR:   A8H5A5
- STRING:   A8H5A5
- GeneID:   5662815
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2422
- NMPDR:   fig|398579.3.peg.2086
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   APWPVDY
- ProtClustDB:   PRK01966
- BioCyc:   SPEA398579:SPEA_2422-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 37223; Mature: 37092

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKNLLLLCGGGGDEHSISLLSANFFEAQLATLPHFNVLRVELNAKGQYHTQAGELCEL
CCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHC
TNSQQLRFDDETKAPWNVDYVIPCIHGYPGETGDIQSYFELINLPYFGCDSESSSNCFNK
CCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHH
VTAKMWFSALGIPNTPYIFLSEFNQQSLEQAEAALAKWGSIFIKAASQGSSVGCYRVDSI
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECHHH
EQLASSLEEAFTFSPYVVIEKTITARELEVAAYEADGEIIATKPGEIICASNTFYSFDEK
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCEEEECCCCCCCHHH
YAENSQAQTVIEADVDEAIAKQIQEYAIKAFKGMKLRHLSRIDFFLTEDNEILLNEINTF
HCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHCCC
PGQTQISMFPKMLQNHGHNFAQYLSGNILAQLKS
CCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEECC
>Mature Secondary Structure 
TQKNLLLLCGGGGDEHSISLLSANFFEAQLATLPHFNVLRVELNAKGQYHTQAGELCEL
CCCCEEEEECCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHC
TNSQQLRFDDETKAPWNVDYVIPCIHGYPGETGDIQSYFELINLPYFGCDSESSSNCFNK
CCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHH
VTAKMWFSALGIPNTPYIFLSEFNQQSLEQAEAALAKWGSIFIKAASQGSSVGCYRVDSI
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECHHH
EQLASSLEEAFTFSPYVVIEKTITARELEVAAYEADGEIIATKPGEIICASNTFYSFDEK
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCCCEEEECCCCCCCHHH
YAENSQAQTVIEADVDEAIAKQIQEYAIKAFKGMKLRHLSRIDFFLTEDNEILLNEINTF
HCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEEHHCCC
PGQTQISMFPKMLQNHGHNFAQYLSGNILAQLKS
CCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA