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The map label for this gene is nadE

Identifier: 157961582

GI number: 157961582

Start: 2137233

End: 2138063

Strand: Direct

Name: nadE

Synonym: Spea_1758

Alternate gene names: 157961582

Gene position: 2137233-2138063 (Clockwise)

Preceding gene: 157961580

Following gene: 157961584

Centisome position: 41.3

GC content: 46.69

Gene sequence:

>831_bases
GTGAAAGGACAGATCCTAAGAGAAATGAAGGTGCTTAACGCTATTGACCCTGGTTTCGAAGTTCAAAGGCGCGTCGCATT
TATTAAGTCAAAGCTTAAGCAATCAAGTACCTCCACTTTAGTCTTGGGTATTAGTGGTGGTGTAGACTCTTCTTTAGCGG
GACGCTTGTGTCAACTAGCCGTTGATGAGCTCAATAGCGACGCCAGCGGTAACCATTACCAATTTATCGCGGTTCGTTTA
CCTTACGATGTGCAAAAGGATGAAGATGAAGCTCAGTTGGCTTGTCAGTTTATCCAACCTTCAAAGCAAGTCACTGTGAA
TGTGAAGCAGGGGGTGGATGGTGTTCATTGTGAAACACTAGCGGCTGTTGAAGCTGCGGGTATCGCGTTACCCGAGGCCG
ATAAAATCGACTTTGTTAAAGGCAACGTAAAGGCAAGAATGCGTATGGTTGCCCAATATGAGATTGCGGGTCTAGTTGCA
GGTTTGGTGGTGGGAACCGACCATAGTGCCGAAAATATTACCGGTTTTTATACCAAGTGGGGCGATGGCGCCTGTGATTT
AGCGCCTTTGTTCGGTCTTAATAAGCGTCAGGTTCGCCAGCTTGCCGCATTCTTAGGTGCGCCAGATAAGTTGGTTATCA
AGGCGCCTACAGCAGATTTAGAAGAGAACAAGCCTCAGTTAGAAGATGAGGTTGCCCTGGGGTTAACCTACGAGCAGATT
GATGACTTTTTAGAGGGTAAAGAGGTCAGCGAATTTGTTAATGACAAACTGGTTGGGATCTACCGCGCCACTCAGCATAA
GCGTGAGCCAATTCCAACGATTTACGATTAA

Upstream 100 bases:

>100_bases
GAAATTAACACGATTGCTATCATTCAGCCCGTGAATTGTCGCAAGAATTCACCCATAATATAAACCGATTTTTCTGCCAA
CTACCATCGGAGTGTAGAAC

Downstream 100 bases:

>100_bases
CATTTCCTACTTCTACAATAAATGGCGCTTCGGCGCCTTTTTCATTTTCAGCCATAAACGCCTTTTTGCAGTAGGTGGAT
GCTTACTTTTAGCCTTTACT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKGQILREMKVLNAIDPGFEVQRRVAFIKSKLKQSSTSTLVLGISGGVDSSLAGRLCQLAVDELNSDASGNHYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKQGVDGVHCETLAAVEAAGIALPEADKIDFVKGNVKARMRMVAQYEIAGLVA
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAFLGAPDKLVIKAPTADLEENKPQLEDEVALGLTYEQI
DDFLEGKEVSEFVNDKLVGIYRATQHKREPIPTIYD

Sequences:

>Translated_276_residues
MKGQILREMKVLNAIDPGFEVQRRVAFIKSKLKQSSTSTLVLGISGGVDSSLAGRLCQLAVDELNSDASGNHYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKQGVDGVHCETLAAVEAAGIALPEADKIDFVKGNVKARMRMVAQYEIAGLVA
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAFLGAPDKLVIKAPTADLEENKPQLEDEVALGLTYEQI
DDFLEGKEVSEFVNDKLVGIYRATQHKREPIPTIYD
>Mature_276_residues
MKGQILREMKVLNAIDPGFEVQRRVAFIKSKLKQSSTSTLVLGISGGVDSSLAGRLCQLAVDELNSDASGNHYQFIAVRL
PYDVQKDEDEAQLACQFIQPSKQVTVNVKQGVDGVHCETLAAVEAAGIALPEADKIDFVKGNVKARMRMVAQYEIAGLVA
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAFLGAPDKLVIKAPTADLEENKPQLEDEVALGLTYEQI
DDFLEGKEVSEFVNDKLVGIYRATQHKREPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=50.9025270758123, Blast_Score=264, Evalue=4e-72,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_SHEPA (A8H3E4)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501616.1
- ProteinModelPortal:   A8H3E4
- SMR:   A8H3E4
- STRING:   A8H3E4
- GeneID:   5662153
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1758
- NMPDR:   fig|398579.3.peg.1514
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   SPEA398579:SPEA_1758-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30122; Mature: 30122

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGQILREMKVLNAIDPGFEVQRRVAFIKSKLKQSSTSTLVLGISGGVDSSLAGRLCQLA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
VDELNSDASGNHYQFIAVRLPYDVQKDEDEAQLACQFIQPSKQVTVNVKQGVDGVHCETL
HHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
AAVEAAGIALPEADKIDFVKGNVKARMRMVAQYEIAGLVAGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAFLGAPDKLVIKAPTADLEENKPQLEDEVALGLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHH
DDFLEGKEVSEFVNDKLVGIYRATQHKREPIPTIYD
HHHHCCHHHHHHHCCHHEEEHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKGQILREMKVLNAIDPGFEVQRRVAFIKSKLKQSSTSTLVLGISGGVDSSLAGRLCQLA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
VDELNSDASGNHYQFIAVRLPYDVQKDEDEAQLACQFIQPSKQVTVNVKQGVDGVHCETL
HHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
AAVEAAGIALPEADKIDFVKGNVKARMRMVAQYEIAGLVAGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAFLGAPDKLVIKAPTADLEENKPQLEDEVALGLTYEQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHH
DDFLEGKEVSEFVNDKLVGIYRATQHKREPIPTIYD
HHHHCCHHHHHHHCCHHEEEHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA