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The map label for this gene is glnG [H]

Identifier: 157961580

GI number: 157961580

Start: 2134969

End: 2136321

Strand: Direct

Name: glnG [H]

Synonym: Spea_1756

Alternate gene names: 157961580

Gene position: 2134969-2136321 (Clockwise)

Preceding gene: 157961579

Following gene: 157961582

Centisome position: 41.26

GC content: 48.19

Gene sequence:

>1353_bases
ATGAATAAAGCGCCGTTACAGATCTTAGTGGTGGAAGACGAGCCCGATCAACGAGAGTTAATTTGTCAAATACTTGCCTC
GAATCAATATCAGATCACCAGCGCTGAAAGCGTTGAAGAGGCGATTTTATCAATAAAGTCGAGTGTACCGGATCTGGTTT
TTTCTGATTGGAAGCTTGGACAGTTAACAGGGATGGATCTGCTGACATACGTAAGACGAGAGCACCCCGATATGGGATTT
ATTATCGCCACGGCTTACGGCACTATTACCCACGCGGTGGACGCCATGCAAGCAGGGGCAGATGATTACCTGTCAAAACC
TTATCAACGTCAGTCGTTACTGATGGCTATAGATAAGGTCGCTAAATCGTTAACGTTAAAACAGCAAAATCGACGTCTAG
CATCAGAGCTATCTCAGCAACAGGAGCTCGTGGGGCTAGTGGGTAAGGCGCCGTGTATGCAGAAGGTTTATGCTCGAGTA
CAAAGAGTGAGTGCCACCGACGCAACGGTGCTAATTGGCGGCGAGAGTGGCACAGGTAAAGAGCTAGCGGCTCGGGCCTT
ATATCAATTATCGAGTCGTCAACATAAGCCGTTTGTCGCTATCAATTGCGGCGCCATTCCCGAAACTCTTGCGGAATCAG
AGCTCTTTGGCGCAGAGAAAGGGGCATTTACTGGTGCCAATACGCTGAAAATCGGTAAGCTTGAGGCGGCTAATGGCGGT
ACTATCTTTCTCGATGAAATAGGCGAGTTACCTCTGTTACAGCAGACAAAGTTGCTCAGGTTTTTACAAGAAGGCGTGAT
TTCTAGGCTAGGGCAAAACGGTGAAATTAAGTTGGATGTTAGGGTCATTGCCGCGACCCACCGCAACCTTAAAGAGGAAG
TCGAGAAGGGCAACTTTAGAGAAGATCTATTCTATAGGCTCAACGTGGTCCCCATCGATATGCCACCACTGAGAGAGCGC
AAAGAAGATATTGGCCGGCTCATAGAGCATTTCTTAAAAATTCATGCAGGTCAGTATGGCGTCGAGGCAGTTAAGTTAAG
TGCGGAAACCATGAAGTCTCTGCTCGATTATCCTTGGCCGGGCAATGTGCGAGAACTCTCTAATCGAGTTGAACGTTTCG
TATTACTGGCAGATGAGCAAGAGCTGGTGGCCGAACTGGCGTCAGGACTGGTCGCGATTAATCCGAGTCAGTTTGTGCTT
CCTGAGGCGGGATTTGAGTGGGAAGCGTTTGAGAAAGACTGCTTACAGCAAGCGATGTCGCGCCATGAGGGGAATCGAAC
CCAAGCGGCAAAGCAGCTAGGCTTGAGTTATAAAGCGTTTTTGTATCGCTTAGAGAAGTATCAACTAGTGTAA

Upstream 100 bases:

>100_bases
TTTGTCGTGGTGAACCAGGGCCTGACTCAAGCGCCAAATCAAACATAGACTCAAACTCTGACTCAAACAGAAAGCCTTCG
TATCAAGGTAAGGTGAAACC

Downstream 100 bases:

>100_bases
CCTTTAGGTATGAGCCGCTTATCATAGCGGCTTGTACTAAAAGTTAGTGCTAAAGGTTAGTGCGATGTCACGAGGCCAGA
GATAATCATCAGGGCCACCC

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNKAPLQILVVEDEPDQRELICQILASNQYQITSAESVEEAILSIKSSVPDLVFSDWKLGQLTGMDLLTYVRREHPDMGF
IIATAYGTITHAVDAMQAGADDYLSKPYQRQSLLMAIDKVAKSLTLKQQNRRLASELSQQQELVGLVGKAPCMQKVYARV
QRVSATDATVLIGGESGTGKELAARALYQLSSRQHKPFVAINCGAIPETLAESELFGAEKGAFTGANTLKIGKLEAANGG
TIFLDEIGELPLLQQTKLLRFLQEGVISRLGQNGEIKLDVRVIAATHRNLKEEVEKGNFREDLFYRLNVVPIDMPPLRER
KEDIGRLIEHFLKIHAGQYGVEAVKLSAETMKSLLDYPWPGNVRELSNRVERFVLLADEQELVAELASGLVAINPSQFVL
PEAGFEWEAFEKDCLQQAMSRHEGNRTQAAKQLGLSYKAFLYRLEKYQLV

Sequences:

>Translated_450_residues
MNKAPLQILVVEDEPDQRELICQILASNQYQITSAESVEEAILSIKSSVPDLVFSDWKLGQLTGMDLLTYVRREHPDMGF
IIATAYGTITHAVDAMQAGADDYLSKPYQRQSLLMAIDKVAKSLTLKQQNRRLASELSQQQELVGLVGKAPCMQKVYARV
QRVSATDATVLIGGESGTGKELAARALYQLSSRQHKPFVAINCGAIPETLAESELFGAEKGAFTGANTLKIGKLEAANGG
TIFLDEIGELPLLQQTKLLRFLQEGVISRLGQNGEIKLDVRVIAATHRNLKEEVEKGNFREDLFYRLNVVPIDMPPLRER
KEDIGRLIEHFLKIHAGQYGVEAVKLSAETMKSLLDYPWPGNVRELSNRVERFVLLADEQELVAELASGLVAINPSQFVL
PEAGFEWEAFEKDCLQQAMSRHEGNRTQAAKQLGLSYKAFLYRLEKYQLV
>Mature_450_residues
MNKAPLQILVVEDEPDQRELICQILASNQYQITSAESVEEAILSIKSSVPDLVFSDWKLGQLTGMDLLTYVRREHPDMGF
IIATAYGTITHAVDAMQAGADDYLSKPYQRQSLLMAIDKVAKSLTLKQQNRRLASELSQQQELVGLVGKAPCMQKVYARV
QRVSATDATVLIGGESGTGKELAARALYQLSSRQHKPFVAINCGAIPETLAESELFGAEKGAFTGANTLKIGKLEAANGG
TIFLDEIGELPLLQQTKLLRFLQEGVISRLGQNGEIKLDVRVIAATHRNLKEEVEKGNFREDLFYRLNVVPIDMPPLRER
KEDIGRLIEHFLKIHAGQYGVEAVKLSAETMKSLLDYPWPGNVRELSNRVERFVLLADEQELVAELASGLVAINPSQFVL
PEAGFEWEAFEKDCLQQAMSRHEGNRTQAAKQLGLSYKAFLYRLEKYQLV

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=39.8286937901499, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=40.6181015452539, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.4955752212389, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=38.7527839643653, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789233, Length=356, Percent_Identity=39.3258426966292, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082117, Length=253, Percent_Identity=50.5928853754941, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1789087, Length=258, Percent_Identity=48.4496124031008, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=42.0731707317073, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786524, Length=360, Percent_Identity=41.9444444444444, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=40.8945686900958, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=36.8421052631579, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789828, Length=258, Percent_Identity=34.8837209302326, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87081858, Length=209, Percent_Identity=33.4928229665072, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=33.0578512396694, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50118; Mature: 50118

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAPLQILVVEDEPDQRELICQILASNQYQITSAESVEEAILSIKSSVPDLVFSDWKLG
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHCCCCCC
QLTGMDLLTYVRREHPDMGFIIATAYGTITHAVDAMQAGADDYLSKPYQRQSLLMAIDKV
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
AKSLTLKQQNRRLASELSQQQELVGLVGKAPCMQKVYARVQRVSATDATVLIGGESGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELAARALYQLSSRQHKPFVAINCGAIPETLAESELFGAEKGAFTGANTLKIGKLEAANGG
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
TIFLDEIGELPLLQQTKLLRFLQEGVISRLGQNGEIKLDVRVIAATHRNLKEEVEKGNFR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHCCCCH
EDLFYRLNVVPIDMPPLRERKEDIGRLIEHFLKIHAGQYGVEAVKLSAETMKSLLDYPWP
HHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCC
GNVRELSNRVERFVLLADEQELVAELASGLVAINPSQFVLPEAGFEWEAFEKDCLQQAMS
CCHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHH
RHEGNRTQAAKQLGLSYKAFLYRLEKYQLV
HCCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKAPLQILVVEDEPDQRELICQILASNQYQITSAESVEEAILSIKSSVPDLVFSDWKLG
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCHHHCCCCCC
QLTGMDLLTYVRREHPDMGFIIATAYGTITHAVDAMQAGADDYLSKPYQRQSLLMAIDKV
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
AKSLTLKQQNRRLASELSQQQELVGLVGKAPCMQKVYARVQRVSATDATVLIGGESGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELAARALYQLSSRQHKPFVAINCGAIPETLAESELFGAEKGAFTGANTLKIGKLEAANGG
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
TIFLDEIGELPLLQQTKLLRFLQEGVISRLGQNGEIKLDVRVIAATHRNLKEEVEKGNFR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHCCCCH
EDLFYRLNVVPIDMPPLRERKEDIGRLIEHFLKIHAGQYGVEAVKLSAETMKSLLDYPWP
HHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCC
GNVRELSNRVERFVLLADEQELVAELASGLVAINPSQFVLPEAGFEWEAFEKDCLQQAMS
CCHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHH
RHEGNRTQAAKQLGLSYKAFLYRLEKYQLV
HCCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]