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The map label for this gene is cca

Identifier: 157960799

GI number: 157960799

Start: 1181112

End: 1182350

Strand: Reverse

Name: cca

Synonym: Spea_0971

Alternate gene names: 157960799

Gene position: 1182350-1181112 (Counterclockwise)

Preceding gene: 157960802

Following gene: 157960798

Centisome position: 22.85

GC content: 48.1

Gene sequence:

>1239_bases
GTGAAAATTTACCTAGTTGGCGGCGCAGTCCGCGATAAATTACTCAAGCTTCCGGTTAAGGACCATGACTACATGGTCGT
TGGCGCAACACCTGAACAGATGCTTAGCCTCGGCTACAACCAGGTTGGTAAAGACTTCCCTGTATTTTTACACCCTAAAA
CAGGGCAAGAGTATGCGCTTGCACGCACCGAACGCAAAACGGCGGCGGGCTATGGTGGCTTTAGTGTCGATGCGGCGCCA
ACTGTCACCCTAGAGCAAGATTTGCTCCGCAGAGATTTAACCATTAACGCCATCGCACAAGATGACGCGGGTAATCTTTA
CGATCCCTATAATGGCATTGCCGATCTTGAGAAGCGCATCCTAAGACATGTCTCTGATGCCTTTGTTGAAGATCCTCTAC
GCGTCCTTAGAGTGGCTCGCTTTGCAGCCCGTTTCCATGGGTTGAAGTTTACCATTGCACCTGAAACCCTTTCACTAATG
ACAACTCTCAGTCGAAGTGGTGAACTAGAGGCATTAACCGCCGAACGCGTCTATTTGGAACTCGATAAGGCGCTTTCTAC
AGATAACCCACAGGTATTCTTTCAAGTATTAAAAGAGTGCGGCGCACTTGCGGTGCTTTTCCCTGAGATTGAGGCCTTGT
TTGGTGTACCTCAGCCAGAAAACTGGCATCCTGAGATAGATACAGGCATTCATACCATGATGGTGCTTGAGCAAGCAGCT
AAGCTAAGCGACGATAACTCGGTACGCTTTGCCGCACTGGTTCATGATCTAGGTAAGGCTTTAAGCCCTAAAGAACATCT
GCCTAAACATCATGGACACGGTCAAAAGGGCTTGCCTTTAATCAAGTCTCTGTGTGAACGCTTTAGGGTGCCCAACGAAT
ATCGTGATCTCGCGCTACTTGTCTGCGACCAGCACCAAAACATCCACAAGATCACCGAACTTAGAGCCGATACTTTAGTT
AAGCTGTTTGATAAAGCTGACTTTTGGCGTAAGCCACAACGACTCGAACAGCTATTAATTGCCTGTGAAGCCGACAGTAA
AGGTCGCAAGGGGCTGGAACAAACCGCCTATCCCCAAGCTGATTATCTTAAGCAATGTTTTGCAGCGGCTTCCGCCGTGG
AGGTTAAGTCTATTATCGCGCAGGGATATAAAGGCGCCGAAATCCGTACACAGTTAAATGTTAAACGGATCGCTGAAGTT
CAGGCTGTAAAAGGCCTGCAGCCAATAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
TTTGCCGCAAGAGAGAACAAGAATTCACCTTATAGCACTATGCTTCTAACCCTATGCTTCCATGCTACACTCGCTTCTAA
GTTATTAAGTGAGAATCCCT

Downstream 100 bases:

>100_bases
AAAACCAAAAAAAATTCACATATTGAGCTAACATTATAGATACCCTAATCATGGTTAATTTGTACTTAGCTTAAAAGTGC
TCAAATTTTCACTAAGTTAT

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKIYLVGGAVRDKLLKLPVKDHDYMVVGATPEQMLSLGYNQVGKDFPVFLHPKTGQEYALARTERKTAAGYGGFSVDAAP
TVTLEQDLLRRDLTINAIAQDDAGNLYDPYNGIADLEKRILRHVSDAFVEDPLRVLRVARFAARFHGLKFTIAPETLSLM
TTLSRSGELEALTAERVYLELDKALSTDNPQVFFQVLKECGALAVLFPEIEALFGVPQPENWHPEIDTGIHTMMVLEQAA
KLSDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVCDQHQNIHKITELRADTLV
KLFDKADFWRKPQRLEQLLIACEADSKGRKGLEQTAYPQADYLKQCFAAASAVEVKSIIAQGYKGAEIRTQLNVKRIAEV
QAVKGLQPIEAK

Sequences:

>Translated_412_residues
MKIYLVGGAVRDKLLKLPVKDHDYMVVGATPEQMLSLGYNQVGKDFPVFLHPKTGQEYALARTERKTAAGYGGFSVDAAP
TVTLEQDLLRRDLTINAIAQDDAGNLYDPYNGIADLEKRILRHVSDAFVEDPLRVLRVARFAARFHGLKFTIAPETLSLM
TTLSRSGELEALTAERVYLELDKALSTDNPQVFFQVLKECGALAVLFPEIEALFGVPQPENWHPEIDTGIHTMMVLEQAA
KLSDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVCDQHQNIHKITELRADTLV
KLFDKADFWRKPQRLEQLLIACEADSKGRKGLEQTAYPQADYLKQCFAAASAVEVKSIIAQGYKGAEIRTQLNVKRIAEV
QAVKGLQPIEAK
>Mature_412_residues
MKIYLVGGAVRDKLLKLPVKDHDYMVVGATPEQMLSLGYNQVGKDFPVFLHPKTGQEYALARTERKTAAGYGGFSVDAAP
TVTLEQDLLRRDLTINAIAQDDAGNLYDPYNGIADLEKRILRHVSDAFVEDPLRVLRVARFAARFHGLKFTIAPETLSLM
TTLSRSGELEALTAERVYLELDKALSTDNPQVFFQVLKECGALAVLFPEIEALFGVPQPENWHPEIDTGIHTMMVLEQAA
KLSDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALLVCDQHQNIHKITELRADTLV
KLFDKADFWRKPQRLEQLLIACEADSKGRKGLEQTAYPQADYLKQCFAAASAVEVKSIIAQGYKGAEIRTQLNVKRIAEV
QAVKGLQPIEAK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=62.6237623762376, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87081691, Length=227, Percent_Identity=30.3964757709251, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=235, Percent_Identity=27.2340425531915, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321016, Length=236, Percent_Identity=29.6610169491525, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_SHEPA (A8H161)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001500833.1
- ProteinModelPortal:   A8H161
- SMR:   A8H161
- STRING:   A8H161
- GeneID:   5661370
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_0971
- NMPDR:   fig|398579.3.peg.847
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   CLSK2401638
- BioCyc:   SPEA398579:SPEA_0971-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45970; Mature: 45970

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDKLLKLPVKDHDYMVVGATPEQMLSLGYNQVGKDFPVFLHPKTGQEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCHHHCCCCCCEEEECCCCCHHHH
ARTERKTAAGYGGFSVDAAPTVTLEQDLLRRDLTINAIAQDDAGNLYDPYNGIADLEKRI
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHVSDAFVEDPLRVLRVARFAARFHGLKFTIAPETLSLMTTLSRSGELEALTAERVYLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCEEEEHHHHHHHH
LDKALSTDNPQVFFQVLKECGALAVLFPEIEALFGVPQPENWHPEIDTGIHTMMVLEQAA
HHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KLSDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALL
HCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEEE
VCDQHQNIHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSKGRKGLEQTAYPQA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCH
DYLKQCFAAASAVEVKSIIAQGYKGAEIRTQLNVKRIAEVQAVKGLQPIEAK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDKLLKLPVKDHDYMVVGATPEQMLSLGYNQVGKDFPVFLHPKTGQEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCHHHCCCCCCEEEECCCCCHHHH
ARTERKTAAGYGGFSVDAAPTVTLEQDLLRRDLTINAIAQDDAGNLYDPYNGIADLEKRI
HHHHHHHHCCCCCEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHVSDAFVEDPLRVLRVARFAARFHGLKFTIAPETLSLMTTLSRSGELEALTAERVYLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCEEEEHHHHHHHH
LDKALSTDNPQVFFQVLKECGALAVLFPEIEALFGVPQPENWHPEIDTGIHTMMVLEQAA
HHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KLSDDNSVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIKSLCERFRVPNEYRDLALL
HCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEEE
VCDQHQNIHKITELRADTLVKLFDKADFWRKPQRLEQLLIACEADSKGRKGLEQTAYPQA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCH
DYLKQCFAAASAVEVKSIIAQGYKGAEIRTQLNVKRIAEVQAVKGLQPIEAK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA