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The map label for this gene is hemL [H]

Identifier: 157960802

GI number: 157960802

Start: 1185605

End: 1186894

Strand: Reverse

Name: hemL [H]

Synonym: Spea_0974

Alternate gene names: 157960802

Gene position: 1186894-1185605 (Counterclockwise)

Preceding gene: 157960803

Following gene: 157960799

Centisome position: 22.94

GC content: 47.36

Gene sequence:

>1290_bases
ATGACTCGTTCTGACGAACTATTTGAACAGGCTAAAAAGACCATCCCAGGTGGCGTTAACTCTCCAGTACGTGCATTCTC
TGGTGTAGGTGGCTCACCACGTTTTATCGAGAAAGCTGATGGCGCTTATATCTTCGATGCCGATGGCAAGAAGTATATCG
ACTATGTAGGTTCTTGGGGCCCGATGATCTTAGGTCACAACCACCCAAAAATTCGTGAAGCGGTATTAAAAGCAGTTGAA
AATGGCCTGTCTTTTGGCGCACCAACAGAACTTGAAATCACTATGGCTGAAAAAGTCATCGAGATGGTTCCGTCAATTGA
ACAAGTTCGTATGGTAAGTTCAGGTACTGAAGCTACCATGAGTGCTATCCGTTTAGCACGTGGCTTCACTAACCGTGACA
AAATCCTTAAGTTTGAAGGTTGCTACCACGGCCACGCTGACTGTTTGCTTGTTAAAGCGGGTTCTGGCGCACTAACACTT
GGCCAGCCAAGCTCTCCAGGTATTCCTGAAGATTTCGCTAAGCACACCCTAACAGCGACCTACAACGATTTAGACTCTGT
TAAGGCTATCTTCGAGCAGTACCCAGAAGAGATCTCTTGTATCATCATAGAGCCAGTTGCTGGCAACATGAACTGTATCC
CGCCAATCGACGGTTTCTTACAAGGTCTACGTGCTATCTGTGATCAGTACGGTGCACTGATGATTATCGATGAAGTCATG
ACAGGTTTCCGCGTATCTAAGAGCGGCGCACAGGGACACTACGGCGTAACACCTGATCTTACTACTCTAGGCAAAGTTAT
CGGTGGCGGCATGCCAGTGGGCGCTTTCGGTGGTCGTAAAGATGTGATGCAGTTTATTGCACCAACTGGCCCTGTTTACC
AAGCGGGTACGCTATCGGGTAACCCAATTGCAATGTCTGCTGGTCTTGCACAGATGGAAGCGCTTTGTGAGGAAGGTGTT
TACGAGCAACTAGCTGCTAAGACTCAGTACATTGCTGAAGGCTTTAAAGCTGCTGCTAACAAGCACGGCATTCCAATGGC
TATCAACTACGTTGGCGGCATGTTCGGTTTCTTCTTCACAAGCGAAGAGACTGTAACTAACTTCGCGCAAGTGACTAAGT
GTGACACTGCACTATTTGCTGAATTCTACCACATGATGTTAGATGAAGGTGTTTACCTTGCTCCTAGCGCCTATGAAGCA
GGTTTCCTATCACTTGCTCACGGTGAAGAAGAGCTTGAAGCAACACTCGCTGCTGCTGATCGCGTATTTGCTAAGCTTGC
AAAGGCTTAA

Upstream 100 bases:

>100_bases
GTTAGGTGTTGACTCTCAGCTTGAGCAATACGAACGTCCAGTTAATTGGTATTCACGAAAAGCTGATGTGTAAATCGGCT
ATAGACTTTAAGGATTAAAA

Downstream 100 bases:

>100_bases
TCTTATACCAATCAGTATAAGCCGCTAAGCGTTAACGCTATCGACAAAAAAGGAACACCTAGGTGTTCCTTTTTTCATTT
GTAAATATGCTCAGCTTAAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLKAVE
NGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGV
YEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTSEETVTNFAQVTKCDTALFAEFYHMMLDEGVYLAPSAYEA
GFLSLAHGEEELEATLAAADRVFAKLAKA

Sequences:

>Translated_429_residues
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLKAVE
NGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGV
YEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTSEETVTNFAQVTKCDTALFAEFYHMMLDEGVYLAPSAYEA
GFLSLAHGEEELEATLAAADRVFAKLAKA
>Mature_428_residues
TRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWGPMILGHNHPKIREAVLKAVEN
GLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATMSAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLG
QPSSPGIPEDFAKHTLTATYNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVMT
GFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSGNPIAMSAGLAQMEALCEEGVY
EQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFTSEETVTNFAQVTKCDTALFAEFYHMMLDEGVYLAPSAYEAG
FLSLAHGEEELEATLAAADRVFAKLAKA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=303, Percent_Identity=28.3828382838284, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI284507298, Length=222, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=70.4225352112676, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=29.5673076923077, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788044, Length=334, Percent_Identity=29.0419161676647, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=30.421686746988, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693181, Length=342, Percent_Identity=28.6549707602339, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787560, Length=304, Percent_Identity=30.5921052631579, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786991, Length=359, Percent_Identity=27.8551532033426, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=391, Percent_Identity=26.8542199488491, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=302, Percent_Identity=28.1456953642384, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=349, Percent_Identity=26.9340974212034, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=150, Percent_Identity=34, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=342, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=327, Percent_Identity=25.3822629969419, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=28.8590604026846, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356575, Length=326, Percent_Identity=27.3006134969325, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45857; Mature: 45726

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHCCCCCCEEECCCCCHHHHHHCCCC
PMILGHNHPKIREAVLKAVENGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSG
HCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGVYEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
SEETVTNFAQVTKCDTALFAEFYHMMLDEGVYLAPSAYEAGFLSLAHGEEELEATLAAAD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHCCHHHHHHHHHHHH
RVFAKLAKA
HHHHHHHCC
>Mature Secondary Structure 
TRSDELFEQAKKTIPGGVNSPVRAFSGVGGSPRFIEKADGAYIFDADGKKYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHCCCCCCEEECCCCCHHHHHHCCCC
PMILGHNHPKIREAVLKAVENGLSFGAPTELEITMAEKVIEMVPSIEQVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTNRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLDSVKAIFEQYPEEISCIIIEPVAGNMNCIPPIDGFLQGLRAICDQYGALMIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSKSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGRKDVMQFIAPTGPVYQAGTLSG
HCCEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMSAGLAQMEALCEEGVYEQLAAKTQYIAEGFKAAANKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
SEETVTNFAQVTKCDTALFAEFYHMMLDEGVYLAPSAYEAGFLSLAHGEEELEATLAAAD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHHHCCHHHHHHHHHHHH
RVFAKLAKA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA