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The map label for this gene is puuB [H]

Identifier: 157960778

GI number: 157960778

Start: 1158843

End: 1160165

Strand: Direct

Name: puuB [H]

Synonym: Spea_0950

Alternate gene names: 157960778

Gene position: 1158843-1160165 (Clockwise)

Preceding gene: 157960777

Following gene: 157960779

Centisome position: 22.39

GC content: 49.28

Gene sequence:

>1323_bases
ATGTCAATAATAATCAATGAAAGACAGGCGGAAACCATGATTACTACACCCCATGCAGATTCATACTATGCGGCGTCGGC
TAACGACAAGACCGAGCGTCCGATGCTACAAGATAATATCGACACCGATATCTGTATTATTGGTGCCGGTTACACTGGAT
TGTCGACCGCACTGCACCTACTCGAGATGGGCTACAAGGTGACAGTGCTTGAGGCGGCGCGCATCGGCTGGGGCGCATCG
GGGCGTAATGGCGGCCAGATAGTTAACAGCTTTAGCCGTGATATTGACTCCATTGAGAAAACCGTTGGCACCGAGGCGGG
TAAGCTGTTTGGCGAGATGGCCTTCGAAGGTGCGCGCATTATTAAAGAGCGAATTAAAAAATATAATATTGACTGCGATC
TGCAAGATGGCGGGGTGTTTGCCGCACTAAATGATAAGCAGATGGGCCACCTGCAAGCGCAAAAATCGCTGTGGGAAAAA
CATGGTCATATGAACCACCTTGAGTTGCTGGATAAAAACGACATCCGTAAAGTAGTGAATACTGAGTCCTATGTTGGCGG
GTTACTCGACAAGAGTGGCGGCCATATTCACCCGCTCAACTTGGCGCTAGGTGAGGCCCAAGCGGTCGAATCACTAGGTG
GTCAAATATTTGAAGGCTCGGCGGTGATTAAGGTCGAAGAGGGCGAAAGTCCAGTGGTTCATACCAGCCATGGCAGTGTT
AAGTCTAAGTTTGTTGTGGTTGCGGGTAATGCCTACCTTGGCGGCTTGATACCTAAGCTACAAGCGAAATCTATGCCTTG
CGGCACGCAAGTGATCACCACTGAACCTTTAGGTGAAGAGCTGGCTAAAAGCCTATTGCCACAAAACTACTGCGTCGAAG
ATTGTAATTACCTGCTCGATTACTTCCGTCTATCTGGTGATAACCGCCTGATTTATGGCGGCGGCGTGGTATATGGCGCT
AGGGATCCAGCTGACATTAAGTCGATCATCGGGCCCAAGATGCTCAAGACCTTTCCGCAACTTAAAGATGTGAAGATTGA
TTACACTTGGACGGGTAATTTCCTGCTGACTCTGTCTCGACTTCCACAAGTAGGGCGCATTAGTAGCAATATTTACTATT
CACAAGGCTGTAGTGGCCATGGCGTTACTTACACTCATTTGGCGGGTAAGATCTTAGCCGAGGCGATAAACGGTCAGGCG
ACTAGATTCGATGCCTTTGCGGGCTTGCCACACTACCCATTCCCTGGCGGGCATATGTTCAAGGTGCCTTTTAGTGCCAT
TGGCGCTTGGTATTACACCATGCGTGACAAGTTAGGTGTGTAA

Upstream 100 bases:

>100_bases
CGTTATCCTTATTTGCTGAAGAGGCGGACCCTGCAACAGGTTATCTCATCGAGAACTAACGATCCGCAGCTTAAGCGTAA
AGCACAAAAGGACAGAAGTT

Downstream 100 bases:

>100_bases
CCTTATCGAGAGTATAGGGAAACCAATCACGTTTACAGCAGTCGAAAGGCGATAAGCGTTTAAGCAAATTAAGGAGTCAC
CCGTGCAGTTAAATGATATG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSIIINERQAETMITTPHADSYYAASANDKTERPMLQDNIDTDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGAS
GRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERIKKYNIDCDLQDGGVFAALNDKQMGHLQAQKSLWEK
HGHMNHLELLDKNDIRKVVNTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGESPVVHTSHGSV
KSKFVVVAGNAYLGGLIPKLQAKSMPCGTQVITTEPLGEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGA
RDPADIKSIIGPKMLKTFPQLKDVKIDYTWTGNFLLTLSRLPQVGRISSNIYYSQGCSGHGVTYTHLAGKILAEAINGQA
TRFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV

Sequences:

>Translated_440_residues
MSIIINERQAETMITTPHADSYYAASANDKTERPMLQDNIDTDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGAS
GRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERIKKYNIDCDLQDGGVFAALNDKQMGHLQAQKSLWEK
HGHMNHLELLDKNDIRKVVNTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGESPVVHTSHGSV
KSKFVVVAGNAYLGGLIPKLQAKSMPCGTQVITTEPLGEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGA
RDPADIKSIIGPKMLKTFPQLKDVKIDYTWTGNFLLTLSRLPQVGRISSNIYYSQGCSGHGVTYTHLAGKILAEAINGQA
TRFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV
>Mature_439_residues
SIIINERQAETMITTPHADSYYAASANDKTERPMLQDNIDTDICIIGAGYTGLSTALHLLEMGYKVTVLEAARIGWGASG
RNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARIIKERIKKYNIDCDLQDGGVFAALNDKQMGHLQAQKSLWEKH
GHMNHLELLDKNDIRKVVNTESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGESPVVHTSHGSVK
SKFVVVAGNAYLGGLIPKLQAKSMPCGTQVITTEPLGEELAKSLLPQNYCVEDCNYLLDYFRLSGDNRLIYGGGVVYGAR
DPADIKSIIGPKMLKTFPQLKDVKIDYTWTGNFLLTLSRLPQVGRISSNIYYSQGCSGHGVTYTHLAGKILAEAINGQAT
RFDAFAGLPHYPFPGGHMFKVPFSAIGAWYYTMRDKLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=62.9107981220657, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47897; Mature: 47766

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIINERQAETMITTPHADSYYAASANDKTERPMLQDNIDTDICIIGAGYTGLSTALHL
CEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHH
LEMGYKVTVLEAARIGWGASGRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARI
HHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKERIKKYNIDCDLQDGGVFAALNDKQMGHLQAQKSLWEKHGHMNHLELLDKNDIRKVVN
HHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHC
TESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGESPVVHTSHGSV
CHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCEEECCCEEEEEECCCCCEEECCCCCC
KSKFVVVAGNAYLGGLIPKLQAKSMPCGTQVITTEPLGEELAKSLLPQNYCVEDCNYLLD
CEEEEEEECCHHHCCCCCCHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
YFRLSGDNRLIYGGGVVYGARDPADIKSIIGPKMLKTFPQLKDVKIDYTWTGNFLLTLSR
HHHCCCCCEEEECCEEEECCCCCHHHHHHHCHHHHHHCCCHHCEEEEEEECCCEEEEEHH
LPQVGRISSNIYYSQGCSGHGVTYTHLAGKILAEAINGQATRFDAFAGLPHYPFPGGHMF
CCCCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCEE
KVPFSAIGAWYYTMRDKLGV
ECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIIINERQAETMITTPHADSYYAASANDKTERPMLQDNIDTDICIIGAGYTGLSTALHL
EEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHH
LEMGYKVTVLEAARIGWGASGRNGGQIVNSFSRDIDSIEKTVGTEAGKLFGEMAFEGARI
HHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKERIKKYNIDCDLQDGGVFAALNDKQMGHLQAQKSLWEKHGHMNHLELLDKNDIRKVVN
HHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHC
TESYVGGLLDKSGGHIHPLNLALGEAQAVESLGGQIFEGSAVIKVEEGESPVVHTSHGSV
CHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCEEECCCEEEEEECCCCCEEECCCCCC
KSKFVVVAGNAYLGGLIPKLQAKSMPCGTQVITTEPLGEELAKSLLPQNYCVEDCNYLLD
CEEEEEEECCHHHCCCCCCHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
YFRLSGDNRLIYGGGVVYGARDPADIKSIIGPKMLKTFPQLKDVKIDYTWTGNFLLTLSR
HHHCCCCCEEEECCEEEECCCCCHHHHHHHCHHHHHHCCCHHCEEEEEEECCCEEEEEHH
LPQVGRISSNIYYSQGCSGHGVTYTHLAGKILAEAINGQATRFDAFAGLPHYPFPGGHMF
CCCCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCEE
KVPFSAIGAWYYTMRDKLGV
ECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]