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The map label for this gene is potI [H]

Identifier: 157960777

GI number: 157960777

Start: 1157865

End: 1158680

Strand: Direct

Name: potI [H]

Synonym: Spea_0949

Alternate gene names: 157960777

Gene position: 1157865-1158680 (Clockwise)

Preceding gene: 157960776

Following gene: 157960778

Centisome position: 22.38

GC content: 48.53

Gene sequence:

>816_bases
ATGAAGAAGTTAAATTTCTCTACCGTTATGCTTTGGGCTGGGATGTTTTTCCTGTATGCGCCAATGTTTATCTTGGTTAT
TTACTCATTTAACGAATCTAAGCTGGTTACCGTTTGGGCTGGATTTTCGACTAAATGGTATGGCGAACTGTTTCGCGATC
AGCAGATCCTCGATGCGGTTTGGACCAGTTTACAGGTGGCGTTTTACGCTTCGACCATGGCGGTGATCTTGGGTACCATG
GCCGCTTTTGTAATGACTAGATTTGCCCGGGGCTGGGGTAAGTTAACCCTATCGAATATGATCACCGCGCCTCTGGTTAT
GCCTGAGGTGATCACAGGTCTGTCTTTGCTACTGCTATTTGTACATATGGCTGACTTCTTTGGCTGGCCTGCTGAGCGTG
GTATGTTGACTGTATGGATAGCCCATTCAACCTTCTGCGCGGCCTATGTGGCGGTAGTCGTATCTTCGCGCTTACGTGAG
ATTGACCGCTCGGTCGAAGAAGCGGCTCAGGATCTTGGCGCGACGCCATTTAAAACCTTCTTCGCCATTACGGTACCTAT
GATCTCGCCGGCCTTGGTGGCTGGTTGGTTATTGTCATTTAGTCTATCTCTTGACGACTTAGTGATCGCCAGCTTTGCAT
CGGGCCCAGGTGCGACAACTTTACCTATGGTGGTGTTCTCGTCTGTGCGCATGGGGGTCTCTCCTAAAATTAATGCACTG
GCTACTTTGATCATTCTTATCGTGTCACTCATCGCATTTATCTCTTGGTACTTCGCACGTCGTGCGGATATTAAGCAGCG
CTCTAGTTCAGCTTAA

Upstream 100 bases:

>100_bases
GGCCGGTGGCCTCCGCGCTTGCGATTGTGATGTTAGGGCTACTGATTATCCCGATAACCCTGTTCCACCGCTATCAGGCT
AAAGAGATGGAGAAGACAGC

Downstream 100 bases:

>100_bases
CCTCATCAGTGTCATTCGGGTATGAAAGGGCTATTGCGCGCTTTCATACCCGTTAGCTCTATCGTTATCCTTATTTGCTG
AAGAGGCGGACCCTGCAACA

Product: ornithine carbamoyltransferase

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKLNFSTVMLWAGMFFLYAPMFILVIYSFNESKLVTVWAGFSTKWYGELFRDQQILDAVWTSLQVAFYASTMAVILGTM
AAFVMTRFARGWGKLTLSNMITAPLVMPEVITGLSLLLLFVHMADFFGWPAERGMLTVWIAHSTFCAAYVAVVVSSRLRE
IDRSVEEAAQDLGATPFKTFFAITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRMGVSPKINAL
ATLIILIVSLIAFISWYFARRADIKQRSSSA

Sequences:

>Translated_271_residues
MKKLNFSTVMLWAGMFFLYAPMFILVIYSFNESKLVTVWAGFSTKWYGELFRDQQILDAVWTSLQVAFYASTMAVILGTM
AAFVMTRFARGWGKLTLSNMITAPLVMPEVITGLSLLLLFVHMADFFGWPAERGMLTVWIAHSTFCAAYVAVVVSSRLRE
IDRSVEEAAQDLGATPFKTFFAITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRMGVSPKINAL
ATLIILIVSLIAFISWYFARRADIKQRSSSA
>Mature_271_residues
MKKLNFSTVMLWAGMFFLYAPMFILVIYSFNESKLVTVWAGFSTKWYGELFRDQQILDAVWTSLQVAFYASTMAVILGTM
AAFVMTRFARGWGKLTLSNMITAPLVMPEVITGLSLLLLFVHMADFFGWPAERGMLTVWIAHSTFCAAYVAVVVSSRLRE
IDRSVEEAAQDLGATPFKTFFAITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRMGVSPKINAL
ATLIILIVSLIAFISWYFARRADIKQRSSSA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=259, Percent_Identity=69.1119691119691, Blast_Score=326, Evalue=9e-91,
Organism=Escherichia coli, GI1787368, Length=244, Percent_Identity=37.7049180327869, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787714, Length=258, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788764, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29942; Mature: 29942

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNFSTVMLWAGMFFLYAPMFILVIYSFNESKLVTVWAGFSTKWYGELFRDQQILDAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
WTSLQVAFYASTMAVILGTMAAFVMTRFARGWGKLTLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADFFGWPAERGMLTVWIAHSTFCAAYVAVVVSSRLREIDRSVEEAAQDLGATPFKTF
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FAITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRMGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILIVSLIAFISWYFARRADIKQRSSSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLNFSTVMLWAGMFFLYAPMFILVIYSFNESKLVTVWAGFSTKWYGELFRDQQILDAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
WTSLQVAFYASTMAVILGTMAAFVMTRFARGWGKLTLSNMITAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHMADFFGWPAERGMLTVWIAHSTFCAAYVAVVVSSRLREIDRSVEEAAQDLGATPFKTF
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FAITVPMISPALVAGWLLSFSLSLDDLVIASFASGPGATTLPMVVFSSVRMGVSPKINAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
ATLIILIVSLIAFISWYFARRADIKQRSSSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]