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The map label for this gene is potH [H]

Identifier: 157960776

GI number: 157960776

Start: 1156963

End: 1157868

Strand: Direct

Name: potH [H]

Synonym: Spea_0948

Alternate gene names: 157960776

Gene position: 1156963-1157868 (Clockwise)

Preceding gene: 157960775

Following gene: 157960777

Centisome position: 22.36

GC content: 46.25

Gene sequence:

>906_bases
ATGAAAAAGCCGTTAAAATTTCGATTACCCAAAGGCCAATGTTGGACCATAGGTGTCCCATACTTTTGGCTACTGATGTT
CTTCGCACTACCTTTCGCTATTGTACTTAAGATCAGTTTCTCAACCCCTATCATCTCGATACCGCCTTACGAGCCATTGT
TTCAGTATGCAGAAGAATCGCTGCAGATCATGCTCAACTTGGGCAATTACTTGCTCATTCTCGATGATTCCTTGTACTAC
AACGCTTATCTAAGTTCACTTAAGATGGCGACTATCTCAACCATAGGCTGTTTACTCATAGGCTATCCGATGGCCTATGC
CATTGCTCGTGCGCCTAAGCGTAACCAAACCATGTTAATCCTGTTGGTGATGTTACCTTCTTGGACCTCTTTCCTTATCC
GGGTTTACGCCTGGATGGGCATATTGAGCAACAATGGTGTGATCAATAACTTGTTGATGTGGACCGGCATTATCTCCGAG
CCTTTGCAGATCTTAAATACCAATACCGCGGTCTATATAGGCATTATCTACAGCTATTTGCCGTTTATGATCTTGCCGCT
GTACGCCAACCTGTCTCAACTCGATGGTAGCTTGCTAGAGGCGGCTGCAGATCTCGGTTCTCGCAGTCTCAATACTTTCT
GGAAGGTGACGCTACCGTTATCGAAAGGCGGGGTGATTGCTGGCTCTATGTTGGTGTTTATTCCTGTGGTAGGTGAGTTT
GTGATCCCAGAGTTACTCGGTGGCCCAGACTCATTGATGATAGGTAAGATTCTTTGGCAAGAGTTCTTCAATAATCGTGA
CTGGCCGGTGGCCTCCGCGCTTGCGATTGTGATGTTAGGGCTACTGATTATCCCGATAACCCTGTTCCACCGCTATCAGG
CTAAAGAGATGGAGAAGACAGCATGA

Upstream 100 bases:

>100_bases
AACCGAGAACGTCGCTCCGATCCACCAACGTGGGAAGAGGCGGTTTTCATTAGCTGGGAGGCCACCAGCGGAGTGGTCCT
TAGAGCATAAGGATTCAATC

Downstream 100 bases:

>100_bases
AGAAGTTAAATTTCTCTACCGTTATGCTTTGGGCTGGGATGTTTTTCCTGTATGCGCCAATGTTTATCTTGGTTATTTAC
TCATTTAACGAATCTAAGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKKPLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISFSTPIISIPPYEPLFQYAEESLQIMLNLGNYLLILDDSLYY
NAYLSSLKMATISTIGCLLIGYPMAYAIARAPKRNQTMLILLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWTGIISE
PLQILNTNTAVYIGIIYSYLPFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
VIPELLGGPDSLMIGKILWQEFFNNRDWPVASALAIVMLGLLIIPITLFHRYQAKEMEKTA

Sequences:

>Translated_301_residues
MKKPLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISFSTPIISIPPYEPLFQYAEESLQIMLNLGNYLLILDDSLYY
NAYLSSLKMATISTIGCLLIGYPMAYAIARAPKRNQTMLILLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWTGIISE
PLQILNTNTAVYIGIIYSYLPFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
VIPELLGGPDSLMIGKILWQEFFNNRDWPVASALAIVMLGLLIIPITLFHRYQAKEMEKTA
>Mature_301_residues
MKKPLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISFSTPIISIPPYEPLFQYAEESLQIMLNLGNYLLILDDSLYY
NAYLSSLKMATISTIGCLLIGYPMAYAIARAPKRNQTMLILLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWTGIISE
PLQILNTNTAVYIGIIYSYLPFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
VIPELLGGPDSLMIGKILWQEFFNNRDWPVASALAIVMLGLLIIPITLFHRYQAKEMEKTA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=294, Percent_Identity=61.5646258503401, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=37.6344086021505, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=32.4675324675325, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33795; Mature: 33795

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISFSTPIISIPPYEPLFQYAEES
CCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCHHHHHHHHHH
LQIMLNLGNYLLILDDSLYYNAYLSSLKMATISTIGCLLIGYPMAYAIARAPKRNQTMLI
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWTGIISEPLQILNTNTAVYIGIIYSYL
EEEHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
VIPELLGGPDSLMIGKILWQEFFNNRDWPVASALAIVMLGLLIIPITLFHRYQAKEMEKT
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MKKPLKFRLPKGQCWTIGVPYFWLLMFFALPFAIVLKISFSTPIISIPPYEPLFQYAEES
CCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCHHHHHHHHHH
LQIMLNLGNYLLILDDSLYYNAYLSSLKMATISTIGCLLIGYPMAYAIARAPKRNQTMLI
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LLVMLPSWTSFLIRVYAWMGILSNNGVINNLLMWTGIISEPLQILNTNTAVYIGIIYSYL
EEEHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PFMILPLYANLSQLDGSLLEAAADLGSRSLNTFWKVTLPLSKGGVIAGSMLVFIPVVGEF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
VIPELLGGPDSLMIGKILWQEFFNNRDWPVASALAIVMLGLLIIPITLFHRYQAKEMEKT
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]