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The map label for this gene is puuA [H]

Identifier: 157960773

GI number: 157960773

Start: 1153008

End: 1154357

Strand: Direct

Name: puuA [H]

Synonym: Spea_0945

Alternate gene names: 157960773

Gene position: 1153008-1154357 (Clockwise)

Preceding gene: 157960772

Following gene: 157960774

Centisome position: 22.28

GC content: 49.41

Gene sequence:

>1350_bases
ATGAAAAAGTTAGTCAGTTATTTAAAAGAACACAAAATTACCGAAGTTGAATGTGTCGTCTGCGATATGACAGGCATTGC
ACGTGGCAAGATTGCGCCTGTTGGCAAGTTTATTGACGAGAAAGGCATGCGTTTGCCAGAAAGTGTGTTGTTGCAGACGG
TAACAGGCGACTATGTCGATGACGAATCTTATGATGCGCTACTCGATGAAGCGGATATCGATTTTGTCTGTGTCCCTGAT
GAAAACGCCGTGTTTAAGCTGCCATGGACCATAGAAGCAACCGCTCAGGTGATCCACGATACCTATGACAAGATGGGTAA
CCCTATCGAGTTGTCTCCTCGTAACCTGCTAAAAAAAGTGCTTAAACTTTATGACGATAAAGGTTGGAAGCCAGTGGTTG
CACCTGAGATGGAGTTTTATTTAACGCGCATTAATGAAGATCCTGATCAACCACTTATTCCGCCAGTTGGTCGCTCGGGC
CGCCAGGAGTCGGGTCGCCAGTCTTTTTCTATCGACGCAGCGAATGAGTACGACCCGCTATTTGAAGACATGTATGACTG
GTGTGAAGAGCAGGGCTTAGATATCGACACCTTAATCCATGAAGAGGGCACCGCGCAGATGGAGATTAACTTCTCCCATG
GTGATGCACTGTCTCTTGCTGACCAAGTCTTTGTATTCAAGCGTACCCTACGCGAAGCCGCGCTTAAGCATAATGTTTGC
GCCACCTTTATGGCGAAACCTGTAACTAATGAGCCCGGCAGTGCGATGCATCTGCATCAGAGTATTGTCGACACTAAATC
GGGCAATAACATCTTCTCCAATGAGGATGGCACTAAGAGCCCGCTGTTTTATAGCTATATTGGCGGTCTGCAAAAATTCA
TTCCTGAGTTATTGCCACTATTTGCGCCAAACGTGAACTCCTTCCGCCGCTTCCTACCGGGCGCTTCGGCTCCGGTTAAC
CTTGAGTGGGGCGAAGAAAACCGCACTTGTGGTCTGCGAATTCCAGAGTCTTCACCGCAAAACTTGCGTATTGAAAACCG
TATCGCAGGCGCCGATGCCAACAGCTACTTGTCGATAGCGGCGAGTCTGTTATGTGGTTACATGGGCATGGTCGATGATG
TTAAGCCATCGACGCCAGTGCAAGGCCGTGCAAATGAGACCCGCGGCGGCGCGGCACTGCCATTAACCCTTGAAGAGGCG
TTAGCCATCATGGCCGAAAGCTCGGCTTGTCGAGAATACTTGGGCGATATCTTCACCAATGGTTATATCGCGGTGAAACA
GGCCGATCTTGAGAACTACCGCCGCGTGATCAGCTCATGGGAGCGTGAATTCCTGCTGCTGACGGTGTAG

Upstream 100 bases:

>100_bases
ATCCGGAGTGGAAGGCACTTGATAGTCCTTTTTATACCGCGATCTTTGAAGCTTTTAATAAAGCATGCCAGCGCCGTGCA
GCAAGCAGAGTGAAATAAAT

Downstream 100 bases:

>100_bases
GTATGAGTTGCAGCGAAGCGTTTTGGGGACGTGTCGCTGCAGTTGAAACTGATTGCAATATGTAAAGTAAGTTATTCATA
CAGATAGCATTTTAATGAGT

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKLVSYLKEHKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDESYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLKLYDDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEEQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHLHQSIVDTKSGNNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLFAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRIAGADANSYLSIAASLLCGYMGMVDDVKPSTPVQGRANETRGGAALPLTLEEA
LAIMAESSACREYLGDIFTNGYIAVKQADLENYRRVISSWEREFLLLTV

Sequences:

>Translated_449_residues
MKKLVSYLKEHKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDESYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLKLYDDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEEQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHLHQSIVDTKSGNNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLFAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRIAGADANSYLSIAASLLCGYMGMVDDVKPSTPVQGRANETRGGAALPLTLEEA
LAIMAESSACREYLGDIFTNGYIAVKQADLENYRRVISSWEREFLLLTV
>Mature_449_residues
MKKLVSYLKEHKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVDDESYDALLDEADIDFVCVPD
ENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKVLKLYDDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSG
RQESGRQSFSIDAANEYDPLFEDMYDWCEEQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTNEPGSAMHLHQSIVDTKSGNNIFSNEDGTKSPLFYSYIGGLQKFIPELLPLFAPNVNSFRRFLPGASAPVN
LEWGEENRTCGLRIPESSPQNLRIENRIAGADANSYLSIAASLLCGYMGMVDDVKPSTPVQGRANETRGGAALPLTLEEA
LAIMAESSACREYLGDIFTNGYIAVKQADLENYRRVISSWEREFLLLTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=447, Percent_Identity=26.1744966442953, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=33.4075723830735, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790301, Length=261, Percent_Identity=32.9501915708812, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50066; Mature: 50066

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVSYLKEHKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVD
CHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DESYDALLDEADIDFVCVPDENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKV
CCCHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LKLYDDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSGRQESGRQSFSIDAANEYDPL
HHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
FEDMYDWCEEQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHHCCCCHHHEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTNEPGSAMHLHQSIVDTKSGNNIFSNEDGTKSPLFYSYIGGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FAPNVNSFRRFLPGASAPVNLEWGEENRTCGLRIPESSPQNLRIENRIAGADANSYLSIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECEECCCCCHHHHHHH
ASLLCGYMGMVDDVKPSTPVQGRANETRGGAALPLTLEEALAIMAESSACREYLGDIFTN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEHHHHHHHHHHHHHHHHHHHHHHCC
GYIAVKQADLENYRRVISSWEREFLLLTV
CEEEEEECCHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKKLVSYLKEHKITEVECVVCDMTGIARGKIAPVGKFIDEKGMRLPESVLLQTVTGDYVD
CHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCC
DESYDALLDEADIDFVCVPDENAVFKLPWTIEATAQVIHDTYDKMGNPIELSPRNLLKKV
CCCHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LKLYDDKGWKPVVAPEMEFYLTRINEDPDQPLIPPVGRSGRQESGRQSFSIDAANEYDPL
HHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
FEDMYDWCEEQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHNVC
HHHHHHHHHHCCCCHHHEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTNEPGSAMHLHQSIVDTKSGNNIFSNEDGTKSPLFYSYIGGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FAPNVNSFRRFLPGASAPVNLEWGEENRTCGLRIPESSPQNLRIENRIAGADANSYLSIA
HCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECEECCCCCHHHHHHH
ASLLCGYMGMVDDVKPSTPVQGRANETRGGAALPLTLEEALAIMAESSACREYLGDIFTN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEHHHHHHHHHHHHHHHHHHHHHHCC
GYIAVKQADLENYRRVISSWEREFLLLTV
CEEEEEECCHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]