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The map label for this gene is puuD [H]

Identifier: 157960772

GI number: 157960772

Start: 1152244

End: 1153005

Strand: Direct

Name: puuD [H]

Synonym: Spea_0944

Alternate gene names: 157960772

Gene position: 1152244-1153005 (Clockwise)

Preceding gene: 157960771

Following gene: 157960773

Centisome position: 22.27

GC content: 48.82

Gene sequence:

>762_bases
ATGTCAGAAGTTGGGCTCGCAGTTGTTGGCGTGACAGCATGTAATATGCAGTTGGGCTTACACCCCTTTAATATAGTGGG
TGAAAAGTATCTTCTAGCAATCGCAGATGCGACACAATCTTGGCCATTGGTTATTCCAGCTTTAGGGCATTGCCCTGCCG
AGGTTGTACTTTCTCGTTTGGACGGAATTTTATTCACCGGCTCCCCCTCAAATATCGAACCGCATCATTTTGACGGCCCC
GCAAGTGATGAGGGAACACATCATGATCCTAAGCGCGATGCGACAACCTTACCTCTGTTAAAAGCGGCAATTGATGCTGG
CGTGCCAGTGCTTGCGATCTGCCGTGGCTTTCAAGAGATGAATGTTGTGTTTGGTGGCAGTTTGCATCAAAAGTTGCATG
AGCTGGGCGGGTTTATTGAGCATAGAGAAGACAAAACGAAATCCGTAGAAGTGCAATATGGTATTTCCCACGAGGTTCAA
ATAGAACCTGGGGGACTGCTTCACGATGCATGGGGCCGCAGCTCTGCAGAGGTGAACTCTGTCCATACCCAAGGCGTAGA
CCGCCTTGGGGTTGGGTTGCGGCCAGAAGCATATGCAACCGATGGTCTGATAGAAGCCTTTTCTGTCAGAAACGCAAAGA
ATTTTGCACTAGGTGTGCAGTGGCATCCGGAGTGGAAGGCACTTGATAGTCCTTTTTATACCGCGATCTTTGAAGCTTTT
AATAAAGCATGCCAGCGCCGTGCAGCAAGCAGAGTGAAATAA

Upstream 100 bases:

>100_bases
TTGAGTGGTAACTGTTCATTATTCAAAATTTCGTAAATCAAGATAAAAACTATACAAAAATAATTCTAAAAATTACGAAG
CAAAGGATTGAAGGTGTTTT

Downstream 100 bases:

>100_bases
ATATGAAAAAGTTAGTCAGTTATTTAAAAGAACACAAAATTACCGAAGTTGAATGTGTCGTCTGCGATATGACAGGCATT
GCACGTGGCAAGATTGCGCC

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSEVGLAVVGVTACNMQLGLHPFNIVGEKYLLAIADATQSWPLVIPALGHCPAEVVLSRLDGILFTGSPSNIEPHHFDGP
ASDEGTHHDPKRDATTLPLLKAAIDAGVPVLAICRGFQEMNVVFGGSLHQKLHELGGFIEHREDKTKSVEVQYGISHEVQ
IEPGGLLHDAWGRSSAEVNSVHTQGVDRLGVGLRPEAYATDGLIEAFSVRNAKNFALGVQWHPEWKALDSPFYTAIFEAF
NKACQRRAASRVK

Sequences:

>Translated_253_residues
MSEVGLAVVGVTACNMQLGLHPFNIVGEKYLLAIADATQSWPLVIPALGHCPAEVVLSRLDGILFTGSPSNIEPHHFDGP
ASDEGTHHDPKRDATTLPLLKAAIDAGVPVLAICRGFQEMNVVFGGSLHQKLHELGGFIEHREDKTKSVEVQYGISHEVQ
IEPGGLLHDAWGRSSAEVNSVHTQGVDRLGVGLRPEAYATDGLIEAFSVRNAKNFALGVQWHPEWKALDSPFYTAIFEAF
NKACQRRAASRVK
>Mature_252_residues
SEVGLAVVGVTACNMQLGLHPFNIVGEKYLLAIADATQSWPLVIPALGHCPAEVVLSRLDGILFTGSPSNIEPHHFDGPA
SDEGTHHDPKRDATTLPLLKAAIDAGVPVLAICRGFQEMNVVFGGSLHQKLHELGGFIEHREDKTKSVEVQYGISHEVQI
EPGGLLHDAWGRSSAEVNSVHTQGVDRLGVGLRPEAYATDGLIEAFSVRNAKNFALGVQWHPEWKALDSPFYTAIFEAFN
KACQRRAASRVK

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=249, Percent_Identity=44.578313253012, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27414; Mature: 27283

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVGLAVVGVTACNMQLGLHPFNIVGEKYLLAIADATQSWPLVIPALGHCPAEVVLSRL
CCCCCEEEEEEEEECEEECCCCHHHCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHH
DGILFTGSPSNIEPHHFDGPASDEGTHHDPKRDATTLPLLKAAIDAGVPVLAICRGFQEM
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHH
NVVFGGSLHQKLHELGGFIEHREDKTKSVEVQYGISHEVQIEPGGLLHDAWGRSSAEVNS
HEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCEEECCCCCCCCHHHH
VHTQGVDRLGVGLRPEAYATDGLIEAFSVRNAKNFALGVQWHPEWKALDSPFYTAIFEAF
HHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHCCHHHHHHHHHH
NKACQRRAASRVK
HHHHHHHHHHCCC
>Mature Secondary Structure 
SEVGLAVVGVTACNMQLGLHPFNIVGEKYLLAIADATQSWPLVIPALGHCPAEVVLSRL
CCCCEEEEEEEEECEEECCCCHHHCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHH
DGILFTGSPSNIEPHHFDGPASDEGTHHDPKRDATTLPLLKAAIDAGVPVLAICRGFQEM
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHH
NVVFGGSLHQKLHELGGFIEHREDKTKSVEVQYGISHEVQIEPGGLLHDAWGRSSAEVNS
HEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCEEECCCCCCCCHHHH
VHTQGVDRLGVGLRPEAYATDGLIEAFSVRNAKNFALGVQWHPEWKALDSPFYTAIFEAF
HHHCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHHHCCHHHHHHHHHH
NKACQRRAASRVK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]