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The map label for this gene is potF [H]

Identifier: 157960774

GI number: 157960774

Start: 1154615

End: 1155715

Strand: Direct

Name: potF [H]

Synonym: Spea_0946

Alternate gene names: 157960774

Gene position: 1154615-1155715 (Clockwise)

Preceding gene: 157960773

Following gene: 157960775

Centisome position: 22.31

GC content: 44.14

Gene sequence:

>1101_bases
ATGAAACTTTTTAAAAAGGCGACCACACTTGCCCTCGTCACCGCAAGTGTAATGGCTTCGACTACCGCATACGCTGAGGA
AGTTGTTCGAGTTTATAACTGGTCAGATTATATTGCCGAAGATACCTTAGAGAACTTCCACAAAGAGACTGGCATCCGTG
TTGTTTATGATGTGTTTGACAGTAATGAAGTGGTTGAAGCTAAGCTACTTTCAGGTCGCTCTGGTTACGATATCGTTGTT
CCATCTAATAGTTTTCTCGCTAAGCAAATTAAAGCGGGTGCCTTCCAGAAGTTAGATTCTAATCAACTACCAAATCATAA
GAATTTAAGCCCAGAGCTAATGACTCAGTTAGAGTCAGCCGATCCGGGTAATCAATACTCTGTACCATACCTTTGGGGAA
CCAATGGTATCGGTTATAACGTCGAAAAAGTTAAAGCTGTACTGGGGGAAGATGCGCCTGTTGATTCTTTAGAGCTTATC
TTCAACCCTAAGTATGCCGAGAAGGTGTCTCAGTGTGGATTGTCTTTGCTTGATAGTGCCGATGAGATGATCCCGATGGC
GCTTATCTATCTTGGGCTGGATCCAAACAGCACTAAAGCGGATGACTTTAAGAAGGCCGGTGAAGTGCTTGCTAAGGTGC
GCCCTTATATCACTTACTTCCACTCATCACGTTATATCACTGACTTAGCGAATGGCGATATCTGTGTGGCATTTGGTTAT
TCTGGCGATATTTTCCAGGCTGCGGATCGCGCCGATGAAGCTGAAAATGGTAATACCATCGAATATTCAATCCCTAAAGA
GGGCTCAAACCTTTGGTTTGATATGTTAGCCATTCCTGCTGATGCGGCTAACGTTAAAAACGCTCACACCTTTATCAATT
ATCTGCTGCGCCCAGAGGTGATTGCGCCTATCACTAACTATGTGGCTTATGCGAACCCGAACGTGCCAGCGTTGCCGTTA
GTGGATGAAGATGTGCGTACTAACACTTCTATCTACCCAACTAAAGAGGTGTTAGATCGTTTATATGTTGGCGATGTGCG
TCCAATGAAATCACAGCGTTCATTAACGCGAGTTTGGACCAAAGTGAAATCAGGCTACTAA

Upstream 100 bases:

>100_bases
TAGCATTGGTGAACAATAAGAACTAAAGCCGACAATACGGCAACACAATGTATGGACAGCAGAGGTCGATTTACTGCTGC
TACGCTACCAAGGAGATAGC

Downstream 100 bases:

>100_bases
GTAGAATAGGGCAAGGTATCGTCTTGCCCTTTTTGATCGGAAAGAAACAGATAAATAGATACAAAGGGCGGAGGTTATCG
CCCTTAGTTGGAGAAGTAAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLFKKATTLALVTASVMASTTAYAEEVVRVYNWSDYIAEDTLENFHKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMTQLESADPGNQYSVPYLWGTNGIGYNVEKVKAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPTKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY

Sequences:

>Translated_366_residues
MKLFKKATTLALVTASVMASTTAYAEEVVRVYNWSDYIAEDTLENFHKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMTQLESADPGNQYSVPYLWGTNGIGYNVEKVKAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPTKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY
>Mature_366_residues
MKLFKKATTLALVTASVMASTTAYAEEVVRVYNWSDYIAEDTLENFHKETGIRVVYDVFDSNEVVEAKLLSGRSGYDIVV
PSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMTQLESADPGNQYSVPYLWGTNGIGYNVEKVKAVLGEDAPVDSLELI
FNPKYAEKVSQCGLSLLDSADEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEVIAPITNYVAYANPNVPALPL
VDEDVRTNTSIYPTKEVLDRLYVGDVRPMKSQRSLTRVWTKVKSGY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=369, Percent_Identity=54.7425474254742, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=33.9233038348083, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1787711, Length=353, Percent_Identity=23.5127478753541, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40578; Mature: 40578

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFKKATTLALVTASVMASTTAYAEEVVRVYNWSDYIAEDTLENFHKETGIRVVYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVVEAKLLSGRSGYDIVVPSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMTQLESA
CCCEEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
DPGNQYSVPYLWGTNGIGYNVEKVKAVLGEDAPVDSLELIFNPKYAEKVSQCGLSLLDSA
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHCH
DEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCEEEEECC
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEV
CCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHH
IAPITNYVAYANPNVPALPLVDEDVRTNTSIYPTKEVLDRLYVGDVRPMKSQRSLTRVWT
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
KVKSGY
HHHCCC
>Mature Secondary Structure
MKLFKKATTLALVTASVMASTTAYAEEVVRVYNWSDYIAEDTLENFHKETGIRVVYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVVEAKLLSGRSGYDIVVPSNSFLAKQIKAGAFQKLDSNQLPNHKNLSPELMTQLESA
CCCEEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
DPGNQYSVPYLWGTNGIGYNVEKVKAVLGEDAPVDSLELIFNPKYAEKVSQCGLSLLDSA
CCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHHCH
DEMIPMALIYLGLDPNSTKADDFKKAGEVLAKVRPYITYFHSSRYITDLANGDICVAFGY
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCCEEEEECC
SGDIFQAADRADEAENGNTIEYSIPKEGSNLWFDMLAIPADAANVKNAHTFINYLLRPEV
CCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHH
IAPITNYVAYANPNVPALPLVDEDVRTNTSIYPTKEVLDRLYVGDVRPMKSQRSLTRVWT
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
KVKSGY
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]