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The map label for this gene is purT [H]

Identifier: 157960699

GI number: 157960699

Start: 1070160

End: 1071335

Strand: Reverse

Name: purT [H]

Synonym: Spea_0871

Alternate gene names: 157960699

Gene position: 1071335-1070160 (Counterclockwise)

Preceding gene: 157960703

Following gene: 157960693

Centisome position: 20.7

GC content: 48.72

Gene sequence:

>1176_bases
ATGATAGGTACAGCTTTATCTGCAAATGCTAAGCGCGCCATGTTGCTTGGCTGTGGAGAACTGGGTAAAGAAGTGGCGAT
CGAGCTTCAACGCTACGGTATAGAAGTCATAGGAGTTGATCGCTACCCTAACGCCCCCGCAATGCAAGTCTCTCACCGCT
CACATGTGATCAATATGCTCGATGCTCTGTCGTTAAAAGCGATCATTGACCTCGAAAAACCTGACTTAGTCATTCCAGAA
CTTGAAGCGATTGCGACGCAAACTCTGGTTGAAATGGAGGCTAAAGGCGTCAATATCGTGCCGACAGCGAAAGCGACCCA
ACTCACTATGGACAGAGAGGGCATACGCCGCCTTGCAGCTGAGACTTTAGACATACCAACCTCGCCATACTTCTTCTGCG
ATACTGAAGCCGAGTTTAACGATGCCGTAGCCACTATCGGTTTGCCTTGTGTGGTAAAACCAGTGATGAGCTCATCGGGT
AAAGGCCAGAGCGTTATCCGCACTCAAGAGCAAATTGAATCAGCCTGGCAATATGCTCAAGAAGGCGGCCGTGCGGGTAA
GGGACGAGTGATTGTAGAAGGCTTTATCCCCTTTGATTACGAGATCACCCTACTCACAATTAGTGCGGTAAATGGTATTC
ACTTCTGCGATGCTATTGGCCATCGTCAGGAAGACGGAGATTACCGTGAATCTTGGCAGCCACAGGCGATGTCTGAAGTT
GTACTTGCCAAGTCACAAGCTATCGGTAAAAAAGTCGTTGAGGCGCTTGGTGGTTATGGTCTGTTTGGGGTTGAGCTGTT
TATCAAAGGCGATGATGTTTACTTTTCTGAGGTTTCGCCGCGCCCCCATGATACCGGTATGGTGACGCTAATTAGCCAAG
ATCTATCTGAGTTTGCCCTACATGTTCGTGCAATCTTGGGGCTGCCAATTAGCAATATTGCCCAGCATGGCCCGAGTGCA
TCGGCGGTGATCTTGAGCGAAGGCACATCGAGTAATATTCAATTCTCAGGTGTGGCCGAAGCATTAGTCGAACCAAATAC
CCAAGTACGTTTATTTGCCAAGCCTGAGATAGATGGCAAACGCCGCTTAGGTGTGACACTTGCTAGAGACATAAATATCG
ATAAGGCCGTCGACAAAGCCCTAAGCGCTGCCAGCAAGATTAATGTCATCCTGTAA

Upstream 100 bases:

>100_bases
AATGAGGGTACAAAAAGTGCCCCTCACTGCATTGCTAACTTGACCGCCTTATCGGTTTAGCAATCGATAACAACCCCTAC
AATCTAAAAAGGTAATCAAA

Downstream 100 bases:

>100_bases
ATAAACACCTGCTAATTAAAAAGCCTCAAATTTTCGCTAAAGGTTTAGCGTTAAATTTGAGGCTTATTTGTTTTATACCT
TCTAGCTAAATTTCACCAGA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIGTALSANAKRAMLLGCGELGKEVAIELQRYGIEVIGVDRYPNAPAMQVSHRSHVINMLDALSLKAIIDLEKPDLVIPE
LEAIATQTLVEMEAKGVNIVPTAKATQLTMDREGIRRLAAETLDIPTSPYFFCDTEAEFNDAVATIGLPCVVKPVMSSSG
KGQSVIRTQEQIESAWQYAQEGGRAGKGRVIVEGFIPFDYEITLLTISAVNGIHFCDAIGHRQEDGDYRESWQPQAMSEV
VLAKSQAIGKKVVEALGGYGLFGVELFIKGDDVYFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPISNIAQHGPSA
SAVILSEGTSSNIQFSGVAEALVEPNTQVRLFAKPEIDGKRRLGVTLARDINIDKAVDKALSAASKINVIL

Sequences:

>Translated_391_residues
MIGTALSANAKRAMLLGCGELGKEVAIELQRYGIEVIGVDRYPNAPAMQVSHRSHVINMLDALSLKAIIDLEKPDLVIPE
LEAIATQTLVEMEAKGVNIVPTAKATQLTMDREGIRRLAAETLDIPTSPYFFCDTEAEFNDAVATIGLPCVVKPVMSSSG
KGQSVIRTQEQIESAWQYAQEGGRAGKGRVIVEGFIPFDYEITLLTISAVNGIHFCDAIGHRQEDGDYRESWQPQAMSEV
VLAKSQAIGKKVVEALGGYGLFGVELFIKGDDVYFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPISNIAQHGPSA
SAVILSEGTSSNIQFSGVAEALVEPNTQVRLFAKPEIDGKRRLGVTLARDINIDKAVDKALSAASKINVIL
>Mature_391_residues
MIGTALSANAKRAMLLGCGELGKEVAIELQRYGIEVIGVDRYPNAPAMQVSHRSHVINMLDALSLKAIIDLEKPDLVIPE
LEAIATQTLVEMEAKGVNIVPTAKATQLTMDREGIRRLAAETLDIPTSPYFFCDTEAEFNDAVATIGLPCVVKPVMSSSG
KGQSVIRTQEQIESAWQYAQEGGRAGKGRVIVEGFIPFDYEITLLTISAVNGIHFCDAIGHRQEDGDYRESWQPQAMSEV
VLAKSQAIGKKVVEALGGYGLFGVELFIKGDDVYFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPISNIAQHGPSA
SAVILSEGTSSNIQFSGVAEALVEPNTQVRLFAKPEIDGKRRLGVTLARDINIDKAVDKALSAASKINVIL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=65.8097686375321, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1786733, Length=201, Percent_Identity=24.8756218905473, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=24.2506811989101, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42142; Mature: 42142

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTALSANAKRAMLLGCGELGKEVAIELQRYGIEVIGVDRYPNAPAMQVSHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECHHHHHHHHH
DALSLKAIIDLEKPDLVIPELEAIATQTLVEMEAKGVNIVPTAKATQLTMDREGIRRLAA
HHHHEEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
ETLDIPTSPYFFCDTEAEFNDAVATIGLPCVVKPVMSSSGKGQSVIRTQEQIESAWQYAQ
HHCCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EGGRAGKGRVIVEGFIPFDYEITLLTISAVNGIHFCDAIGHRQEDGDYRESWQPQAMSEV
HCCCCCCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCHHHHHH
VLAKSQAIGKKVVEALGGYGLFGVELFIKGDDVYFSEVSPRPHDTGMVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEHHHHHHHHHH
HVRAILGLPISNIAQHGPSASAVILSEGTSSNIQFSGVAEALVEPNTQVRLFAKPEIDGK
HHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCC
RRLGVTLARDINIDKAVDKALSAASKINVIL
CEECEEEEECCCHHHHHHHHHHHHHHCEEEC
>Mature Secondary Structure
MIGTALSANAKRAMLLGCGELGKEVAIELQRYGIEVIGVDRYPNAPAMQVSHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECHHHHHHHHH
DALSLKAIIDLEKPDLVIPELEAIATQTLVEMEAKGVNIVPTAKATQLTMDREGIRRLAA
HHHHEEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
ETLDIPTSPYFFCDTEAEFNDAVATIGLPCVVKPVMSSSGKGQSVIRTQEQIESAWQYAQ
HHCCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EGGRAGKGRVIVEGFIPFDYEITLLTISAVNGIHFCDAIGHRQEDGDYRESWQPQAMSEV
HCCCCCCCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCHHHHHH
VLAKSQAIGKKVVEALGGYGLFGVELFIKGDDVYFSEVSPRPHDTGMVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEHHHHHHHHHH
HVRAILGLPISNIAQHGPSASAVILSEGTSSNIQFSGVAEALVEPNTQVRLFAKPEIDGK
HHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCC
RRLGVTLARDINIDKAVDKALSAASKINVIL
CEECEEEEECCCHHHHHHHHHHHHHHCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA