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The map label for this gene is lptD [H]

Identifier: 157960693

GI number: 157960693

Start: 1063370

End: 1065694

Strand: Reverse

Name: lptD [H]

Synonym: Spea_0865

Alternate gene names: 157960693

Gene position: 1065694-1063370 (Counterclockwise)

Preceding gene: 157960699

Following gene: 157960692

Centisome position: 20.59

GC content: 45.25

Gene sequence:

>2325_bases
ATGCAGATCCGTTATTTTCTGGCCTTGAGCCTGCTTCCCCAATTAGTCTTAGCAGACGAAACCGAAACTGTAGCAGCGTC
GAGCTCACAGTGCGTCGTTGTACCACCAGTAAAGCCTTACGTTCCACTTTACGAGATGAATACTCCTGCTGATCTGGAGC
GGATCTATATCAGTTCCGACCGCTCTGCGGCGCAGATGGGCAAACAAGCGACCTTCAATGGTGATGTAAACTTCAGTCAA
GGTGGTCGAAATATCGCTGCGGATGAAGCGATTCTAGACCAAGAGTCTGAAGAGCTGCATGCCAATGGCAACCTAGTCTT
CCAAGATGAGATATTTACAGTCACCGCCGACTCTTTAGTAGCTAAGATGCGTAGCAACAGTGCAGTCCTAAGTGGGGCGC
AATATTGGCTCACCGGACAACAGATCCACGGTGATGCTGACAGGCTAGAGATCACCAAAGAAAATAACCTGCTGCTATCT
GGCACTAACTTCACTACCTGCCCGGGCCAAGATCCTGACTGGCTGCTTGAAGCCGACCGCATCAAGATAGACAGTACCGA
GGAGTGGGGAGAAATTTGGGACGCTAAGATTAAAATTGCTGGCGTGCCGGTTATGTATATTCCTTACATGACTATTCCGG
TATCGGACAAACGCAAATCTGGCTTCTTATTTCCATCATTTAGCACCAGCACCACTAATGGTGTTGAGGTAGCCACACCT
TATTATTGGAATATCGCACCAGAATATGACTTAACGTTCACCCCCAACTATATGTCAGCACGAGGTCTATTCACTAAGAC
CGAGTTTAGATATTTAGCCGGAGAGAAACAGCAAGGTCAATTAAACATTGAATACCTTGCCGATGACGACAAGCTTGTCA
ATAGCCCAGATAGATACCTATATCACTGGGAGCACAGCGGCGCGATAAATAAGAACTGGCGCGTCTTGGCAAACTATACC
GATGTCTCGGATAACAACTACTTTAATGACCTAAGTTCTGACGTTCAGCGTGCGACTGATAACCAACTCACCCGCATCGG
TGAAATCAGTTATTTTGAACAAAACTGGGATTTCAGTGCCCGCGTACAAGACATTAAAGTGCTTGGCGAGGAGGAGAAAC
CCTATCAGGTGATGCCGCAGTTTACCTTTAACTACCGCGCCCCAAGCTACTGGCACGGCCTAGATTTTAACCTTTATAGT
GAAGCCACAAACTTCGAGCATCAAGATAGCGAATACTCGACTGCGACTCGTGTTCACTTAGAGCCAAGCATCTCCTATCC
GATCCACGGACCAGCAGGGTCATTGACCAGTGAAGTAAAACTACTACAGACCAACTACTGGCAAGATGATAAATCAAATC
TTTCCAATTCTGAGCTTTCAGAACATGTGAACCGTACTCTGCCACAGGTGCGTATTCATGGTCAGGTCAATTTTGAACGC
TTTACCGACTATTTTGACACCAACTATCGTCAAACTCTTGAACCGCAGTTTCAATACCTTTACGTAGGTTACGAAGATCA
ATCGGACATAGGTATTTACGATACAGCCCTGCTGCAAGAAGATTACAACGGCCTATTTAGGGATCGTCGTTTCTCTGGCC
TTGACCGAATAGCCGATGCTAATCAATTTACATTAGGTTTAACCACTAAGCTGTTTAACGAACAAAACCGTGAAGTGTTT
AAGTTCAGCTTGGGCCAGATTATCTATTTAGAAGACAGCCGTGTTGGCATCAATGATATCACAGGCAGCAACGATACCTT
TGTGCAAGAGAACACATCTAACTCCGCGATTGCCGCGGAATTGACGACCCAACTCTATTCTGACTGGTTCTTAAGTGGAT
CGATTCAATATGATGCTAAAGAGAGCGAAAACAAGAAGAGTGAAGTTACGGTAGACTATCGTCCAGGCACCAATAAGCTT
TTGCAAGCTAGCTATCGTTACGTTCCGGATCTGCTAAATACCAACACCAACGAATCGGTAGATATTAAGCAGATTGGCTT
CCGTGGAGCTTGGCCAATTAATGACAGTTTATATTTGGTGGGTAACTGGTACTACGATCTAAATGAAAGCCGAGCGGTAG
AAACCTACACAGGTTTTCAGTATGAATCATGTTGCTGGGCTGTACGTTTGAGTTATCATTACCGCATTAAGACCAATTAT
ATTGACGAAGATAACCCGGTACAGGATACTCGTGAGCTATTTGAGAGCGGGATCTACCTAAACTTTGTCTTAAAAGGACT
CGGTGGTTCTGGCCCATTAGGTGTGTCAGATATGTTGGATGACGGTCTATTTAACTACCGTAAGCCACTTTATTTGAAGA
ATTAG

Upstream 100 bases:

>100_bases
CAGCTAAGCAATTGAAAAAATTTATTTGGCTGTGTTGCCCTTATCGGCAACCTACTCCTCTTCACTGTCCGACTAAGAAA
TCATAATTAATTGACCTAAG

Downstream 100 bases:

>100_bases
CAAATAAATAACTTAAAAAAATGCTGAACCTAGCTAACGACATGCAGTCAAAGCAGGACTAAAGAATCATTAACCCGGTA
TGTAAATACCGTAAATGACA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MQIRYFLALSLLPQLVLADETETVAASSSQCVVVPPVKPYVPLYEMNTPADLERIYISSDRSAAQMGKQATFNGDVNFSQ
GGRNIAADEAILDQESEELHANGNLVFQDEIFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDADRLEITKENNLLLS
GTNFTTCPGQDPDWLLEADRIKIDSTEEWGEIWDAKIKIAGVPVMYIPYMTIPVSDKRKSGFLFPSFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQQGQLNIEYLADDDKLVNSPDRYLYHWEHSGAINKNWRVLANYT
DVSDNNYFNDLSSDVQRATDNQLTRIGEISYFEQNWDFSARVQDIKVLGEEEKPYQVMPQFTFNYRAPSYWHGLDFNLYS
EATNFEHQDSEYSTATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDKSNLSNSELSEHVNRTLPQVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYEDQSDIGIYDTALLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDITGSNDTFVQENTSNSAIAAELTTQLYSDWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQIGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFVLKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN

Sequences:

>Translated_774_residues
MQIRYFLALSLLPQLVLADETETVAASSSQCVVVPPVKPYVPLYEMNTPADLERIYISSDRSAAQMGKQATFNGDVNFSQ
GGRNIAADEAILDQESEELHANGNLVFQDEIFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDADRLEITKENNLLLS
GTNFTTCPGQDPDWLLEADRIKIDSTEEWGEIWDAKIKIAGVPVMYIPYMTIPVSDKRKSGFLFPSFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQQGQLNIEYLADDDKLVNSPDRYLYHWEHSGAINKNWRVLANYT
DVSDNNYFNDLSSDVQRATDNQLTRIGEISYFEQNWDFSARVQDIKVLGEEEKPYQVMPQFTFNYRAPSYWHGLDFNLYS
EATNFEHQDSEYSTATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDKSNLSNSELSEHVNRTLPQVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYEDQSDIGIYDTALLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDITGSNDTFVQENTSNSAIAAELTTQLYSDWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQIGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFVLKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
>Mature_774_residues
MQIRYFLALSLLPQLVLADETETVAASSSQCVVVPPVKPYVPLYEMNTPADLERIYISSDRSAAQMGKQATFNGDVNFSQ
GGRNIAADEAILDQESEELHANGNLVFQDEIFTVTADSLVAKMRSNSAVLSGAQYWLTGQQIHGDADRLEITKENNLLLS
GTNFTTCPGQDPDWLLEADRIKIDSTEEWGEIWDAKIKIAGVPVMYIPYMTIPVSDKRKSGFLFPSFSTSTTNGVEVATP
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQQGQLNIEYLADDDKLVNSPDRYLYHWEHSGAINKNWRVLANYT
DVSDNNYFNDLSSDVQRATDNQLTRIGEISYFEQNWDFSARVQDIKVLGEEEKPYQVMPQFTFNYRAPSYWHGLDFNLYS
EATNFEHQDSEYSTATRVHLEPSISYPIHGPAGSLTSEVKLLQTNYWQDDKSNLSNSELSEHVNRTLPQVRIHGQVNFER
FTDYFDTNYRQTLEPQFQYLYVGYEDQSDIGIYDTALLQEDYNGLFRDRRFSGLDRIADANQFTLGLTTKLFNEQNREVF
KFSLGQIIYLEDSRVGINDITGSNDTFVQENTSNSAIAAELTTQLYSDWFLSGSIQYDAKESENKKSEVTVDYRPGTNKL
LQASYRYVPDLLNTNTNESVDIKQIGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
IDEDNPVQDTRELFESGIYLNFVLKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=800, Percent_Identity=34.875, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88552; Mature: 88552

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRYFLALSLLPQLVLADETETVAASSSQCVVVPPVKPYVPLYEMNTPADLERIYISSD
CEEEEHHHHHHHHHHEECCCCCCEECCCCCEEEECCCCCCCEEEECCCCCCEEEEEEECC
RSAAQMGKQATFNGDVNFSQGGRNIAADEAILDQESEELHANGNLVFQDEIFTVTADSLV
CHHHHCCCCEEECCCCEECCCCCCCCCCHHHHCCCCHHHCCCCCEEEECCEEEEEHHHHH
AKMRSNSAVLSGAQYWLTGQQIHGDADRLEITKENNLLLSGTNFTTCPGQDPDWLLEADR
HHHCCCCEEECCCEEEEECCEECCCCHHEEEEECCCEEEECCCEEECCCCCCCCEEEECE
IKIDSTEEWGEIWDAKIKIAGVPVMYIPYMTIPVSDKRKSGFLFPSFSTSTTNGVEVATP
EEECCCHHHHHHCCCEEEEECCCEEEECEEEEECCCCCCCCEEEECCCCCCCCCEEEECC
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQQGQLNIEYLADDDKLVNSPDRYL
EEEECCCCCCEEECCCCCCCCCCEEECHHHEECCCCCCCEEEEEEEECCCHHCCCCCCEE
YHWEHSGAINKNWRVLANYTDVSDNNYFNDLSSDVQRATDNQLTRIGEISYFEQNWDFSA
EEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHCCEEEEECCCCCCC
RVQDIKVLGEEEKPYQVMPQFTFNYRAPSYWHGLDFNLYSEATNFEHQDSEYSTATRVHL
EEEEEEEECCCCCCEEECCCEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCEEEEEEE
EPSISYPIHGPAGSLTSEVKLLQTNYWQDDKSNLSNSELSEHVNRTLPQVRIHGQVNFER
CCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECHHH
FTDYFDTNYRQTLEPQFQYLYVGYEDQSDIGIYDTALLQEDYNGLFRDRRFSGLDRIADA
HHHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHCCC
NQFTLGLTTKLFNEQNREVFKFSLGQIIYLEDSRVGINDITGSNDTFVQENTSNSAIAAE
CEEEEEEEHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEHHH
LTTQLYSDWFLSGSIQYDAKESENKKSEVTVDYRPGTNKLLQASYRYVPDLLNTNTNESV
HHHHHHHHHEECCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
DIKQIGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
EHEECCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEEEEEEECC
IDEDNPVQDTRELFESGIYLNFVLKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCHHHCCCCEEECC
>Mature Secondary Structure
MQIRYFLALSLLPQLVLADETETVAASSSQCVVVPPVKPYVPLYEMNTPADLERIYISSD
CEEEEHHHHHHHHHHEECCCCCCEECCCCCEEEECCCCCCCEEEECCCCCCEEEEEEECC
RSAAQMGKQATFNGDVNFSQGGRNIAADEAILDQESEELHANGNLVFQDEIFTVTADSLV
CHHHHCCCCEEECCCCEECCCCCCCCCCHHHHCCCCHHHCCCCCEEEECCEEEEEHHHHH
AKMRSNSAVLSGAQYWLTGQQIHGDADRLEITKENNLLLSGTNFTTCPGQDPDWLLEADR
HHHCCCCEEECCCEEEEECCEECCCCHHEEEEECCCEEEECCCEEECCCCCCCCEEEECE
IKIDSTEEWGEIWDAKIKIAGVPVMYIPYMTIPVSDKRKSGFLFPSFSTSTTNGVEVATP
EEECCCHHHHHHCCCEEEEECCCEEEECEEEEECCCCCCCCEEEECCCCCCCCCEEEECC
YYWNIAPEYDLTFTPNYMSARGLFTKTEFRYLAGEKQQGQLNIEYLADDDKLVNSPDRYL
EEEECCCCCCEEECCCCCCCCCCEEECHHHEECCCCCCCEEEEEEEECCCHHCCCCCCEE
YHWEHSGAINKNWRVLANYTDVSDNNYFNDLSSDVQRATDNQLTRIGEISYFEQNWDFSA
EEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHCCEEEEECCCCCCC
RVQDIKVLGEEEKPYQVMPQFTFNYRAPSYWHGLDFNLYSEATNFEHQDSEYSTATRVHL
EEEEEEEECCCCCCEEECCCEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCEEEEEEE
EPSISYPIHGPAGSLTSEVKLLQTNYWQDDKSNLSNSELSEHVNRTLPQVRIHGQVNFER
CCCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECHHH
FTDYFDTNYRQTLEPQFQYLYVGYEDQSDIGIYDTALLQEDYNGLFRDRRFSGLDRIADA
HHHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHCCC
NQFTLGLTTKLFNEQNREVFKFSLGQIIYLEDSRVGINDITGSNDTFVQENTSNSAIAAE
CEEEEEEEHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEHHH
LTTQLYSDWFLSGSIQYDAKESENKKSEVTVDYRPGTNKLLQASYRYVPDLLNTNTNESV
HHHHHHHHHEECCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
DIKQIGFRGAWPINDSLYLVGNWYYDLNESRAVETYTGFQYESCCWAVRLSYHYRIKTNY
EHEECCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEEEEEEECC
IDEDNPVQDTRELFESGIYLNFVLKGLGGSGPLGVSDMLDDGLFNYRKPLYLKN
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA