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The map label for this gene is btuB [H]

Identifier: 157960541

GI number: 157960541

Start: 876103

End: 878292

Strand: Reverse

Name: btuB [H]

Synonym: Spea_0713

Alternate gene names: 157960541

Gene position: 878292-876103 (Counterclockwise)

Preceding gene: 157960548

Following gene: 157960540

Centisome position: 16.97

GC content: 47.26

Gene sequence:

>2190_bases
ATGTCACACCGTAATACGCGATCTTTAAGTCTAATTTCAACTGCGTTACTCGCCGCGCTCTCACCTCAAGTCATGGCACA
GGAGTCTGACTCCCAACCGACCATGGAACAGATCAGTATTTTTGGTAAACAGAATCCGCTCAATACCGCTCCGGGCAGTG
CGCACCTCATCACTCAAGCCGAGCTAGATAACTTCAAATACACAGATATCATGCGCACACTTGCGAGCATTCCCGGTGTG
TATATTCAAGAAGAAGATGGTTACGGTTTAAGACCAAACATAGGTATGCGAGGCACTGGGCAAAATCGCTCAGAAAAAAT
CACCATCATGGAGGACGGTGTACTCGCTGCGCCGGCTCCCTACGCGTCACCTGCGGCCTATTACTTCCCGACTCCGGGCA
GAATGCAATCAGTTGAGATCCTAAAAGGAAGTTCGACCGTTAAATATGGGCCAAGGACCACAGGCGGCGTGATCAATATG
CTATCTCGCCAAATCCCCGAAGAGGAGTTAGCGGGTAAGCTAGATGTCGCTGCAGGTCAAGATGGCTTCGGAAAGTTACA
TGCCTACGCTGGCGGCCAAGGAGAGCGAGTCGGGGCGGTAATGGAAATGTACCGCTACCAAGCCGATGGCTTTAAGGATA
TCAATGGCGTGGGTGGCGACACAGGTTTTGAAAAGAACGACGCGCTGGCAAAGGTCAGTGTGCGCTCGGCTGACGATGCT
AAATACGCTCAGGTGTTAGAGTTTAAGCTGAAATACTCTGACGAAGTCTCCAATGAAACCTATATGGGATTAACCGATAG
TGATTTAGAAACGACACCCTATGCTCGTTACTCGGCCTCACAAAAAGATGTGATGAATACTGAGCATAAACAGTTACAGA
TCAACCATATTATCGACTTTAGCGATAATCTCACCTTAGGGACGACAGCCTACTATAACGATTTTGCTCGCAACTGGTAT
AAGGCCGATAAAGTTAATGGTCTTAAACTCAGTAAAGGAGGGGTTGAAGCCGCCTCTGAATTTGATAAAGATCCATCTAT
GGCTCCAATGGACGTAAGAGTTAAAGCCAACAATCGAAGCTATATTTCACAGGGCGTTCAAACGGAACTGAACTGGTATT
TAGCTAATCATGATCTCGCTTTCGGTGCTCGCTACCATGAAGATGAGATGGACCGATTCCAATGGGCCGATCACTATACT
CTAGACTCAAATCAGGTGATGACCTTAAGTGAAAGCGGTATTCCAGGCACCGACTCTAACCGTATCGACAGTGCCAAGGC
CATTGCTCTTTACGTTCAAGATAAGATCCAGTTCGGCGACTTCAACATCACTGCAGGTCTTCGCTACGAAGATGTAACGA
CTAACCGTACCGACTGGGGCAAGGAAAATCCGGGACGTGACGGCCAGCCAAATAAAGATATCGACAACAGTTTTTCTGCG
ACCTTGCCTTCTCTATCTGCAACCTACCAAATAAACAGCGAGCTTCTGGTTCTTGCTGGCGTTCAAAAGGGCTTTGCCCC
CGCCTCTCCAGGCAATGCCGATAAAAAAGAGGAGCAGAGCTGGAACTATGAAGCCGGAGCACGTTATAACAGCGGTGAGT
TTAGCTCAGAAGCCATCTTCTTCTACAGTGATTACAGCAACATGCATGGTGAATGTACCGCATCAAAAGGCTGCGATGAT
GACAATATCGGTGATCAGTATAATGGTGGTGAAGTGGATGTTAAGGGACTGGAGTTAAGTCTTGGCTATAGCTTTAACAC
TCAAACTGATTTCAGTTTTCCAGTGAAACTGGCCTACACCTATACCGATGCGCAGTTTGCCAATAGCTTCGAGTCAGACT
TCGATCCTTGGGGTGATGTCAGCGAGGGAGACAAACTCCCCTATATCGCTGAAAATATGCTGTTTGCATCGGCAGGGGTG
AAAGCTGATAGCTGGGAGCTGACTTTAGCTGCCCGTTACACAGACGATATTCGCACACAAGCAGGCCAAGGCGAAATTGC
AGCCGACAATCTAATTAAGGCCAAGACTATTGTCGATTTATCTGGGCGTTACTTCATCGATGATGCACAAGAGCTTTACC
TTACTGCGGAAAACTTATTCGATGAAAAATATGTCACCTCTCGAGTCTATGGTTCCAACTTCGTAGGCAAACCTATGACG
GTATCCGTAGGATATGCGTATAAATTTTAA

Upstream 100 bases:

>100_bases
GTTAATTTTAATGAACTTGGCATAGTATCCACGCAAATGATAATCAATCCCATTAACATTTAGTGGGTAATTCGAAATTT
CTATCCTATACGGAGCTCCT

Downstream 100 bases:

>100_bases
GCGATAAAGACGGGTTTATTAAACAAAACTTAAAATTAGATTAATTTTTTTCTTTTAAAAAAGGTGAGCCTGAGGCTCAC
CTTTTTTTATGCCACTGAAG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MSHRNTRSLSLISTALLAALSPQVMAQESDSQPTMEQISIFGKQNPLNTAPGSAHLITQAELDNFKYTDIMRTLASIPGV
YIQEEDGYGLRPNIGMRGTGQNRSEKITIMEDGVLAAPAPYASPAAYYFPTPGRMQSVEILKGSSTVKYGPRTTGGVINM
LSRQIPEEELAGKLDVAAGQDGFGKLHAYAGGQGERVGAVMEMYRYQADGFKDINGVGGDTGFEKNDALAKVSVRSADDA
KYAQVLEFKLKYSDEVSNETYMGLTDSDLETTPYARYSASQKDVMNTEHKQLQINHIIDFSDNLTLGTTAYYNDFARNWY
KADKVNGLKLSKGGVEAASEFDKDPSMAPMDVRVKANNRSYISQGVQTELNWYLANHDLAFGARYHEDEMDRFQWADHYT
LDSNQVMTLSESGIPGTDSNRIDSAKAIALYVQDKIQFGDFNITAGLRYEDVTTNRTDWGKENPGRDGQPNKDIDNSFSA
TLPSLSATYQINSELLVLAGVQKGFAPASPGNADKKEEQSWNYEAGARYNSGEFSSEAIFFYSDYSNMHGECTASKGCDD
DNIGDQYNGGEVDVKGLELSLGYSFNTQTDFSFPVKLAYTYTDAQFANSFESDFDPWGDVSEGDKLPYIAENMLFASAGV
KADSWELTLAARYTDDIRTQAGQGEIAADNLIKAKTIVDLSGRYFIDDAQELYLTAENLFDEKYVTSRVYGSNFVGKPMT
VSVGYAYKF

Sequences:

>Translated_729_residues
MSHRNTRSLSLISTALLAALSPQVMAQESDSQPTMEQISIFGKQNPLNTAPGSAHLITQAELDNFKYTDIMRTLASIPGV
YIQEEDGYGLRPNIGMRGTGQNRSEKITIMEDGVLAAPAPYASPAAYYFPTPGRMQSVEILKGSSTVKYGPRTTGGVINM
LSRQIPEEELAGKLDVAAGQDGFGKLHAYAGGQGERVGAVMEMYRYQADGFKDINGVGGDTGFEKNDALAKVSVRSADDA
KYAQVLEFKLKYSDEVSNETYMGLTDSDLETTPYARYSASQKDVMNTEHKQLQINHIIDFSDNLTLGTTAYYNDFARNWY
KADKVNGLKLSKGGVEAASEFDKDPSMAPMDVRVKANNRSYISQGVQTELNWYLANHDLAFGARYHEDEMDRFQWADHYT
LDSNQVMTLSESGIPGTDSNRIDSAKAIALYVQDKIQFGDFNITAGLRYEDVTTNRTDWGKENPGRDGQPNKDIDNSFSA
TLPSLSATYQINSELLVLAGVQKGFAPASPGNADKKEEQSWNYEAGARYNSGEFSSEAIFFYSDYSNMHGECTASKGCDD
DNIGDQYNGGEVDVKGLELSLGYSFNTQTDFSFPVKLAYTYTDAQFANSFESDFDPWGDVSEGDKLPYIAENMLFASAGV
KADSWELTLAARYTDDIRTQAGQGEIAADNLIKAKTIVDLSGRYFIDDAQELYLTAENLFDEKYVTSRVYGSNFVGKPMT
VSVGYAYKF
>Mature_728_residues
SHRNTRSLSLISTALLAALSPQVMAQESDSQPTMEQISIFGKQNPLNTAPGSAHLITQAELDNFKYTDIMRTLASIPGVY
IQEEDGYGLRPNIGMRGTGQNRSEKITIMEDGVLAAPAPYASPAAYYFPTPGRMQSVEILKGSSTVKYGPRTTGGVINML
SRQIPEEELAGKLDVAAGQDGFGKLHAYAGGQGERVGAVMEMYRYQADGFKDINGVGGDTGFEKNDALAKVSVRSADDAK
YAQVLEFKLKYSDEVSNETYMGLTDSDLETTPYARYSASQKDVMNTEHKQLQINHIIDFSDNLTLGTTAYYNDFARNWYK
ADKVNGLKLSKGGVEAASEFDKDPSMAPMDVRVKANNRSYISQGVQTELNWYLANHDLAFGARYHEDEMDRFQWADHYTL
DSNQVMTLSESGIPGTDSNRIDSAKAIALYVQDKIQFGDFNITAGLRYEDVTTNRTDWGKENPGRDGQPNKDIDNSFSAT
LPSLSATYQINSELLVLAGVQKGFAPASPGNADKKEEQSWNYEAGARYNSGEFSSEAIFFYSDYSNMHGECTASKGCDDD
NIGDQYNGGEVDVKGLELSLGYSFNTQTDFSFPVKLAYTYTDAQFANSFESDFDPWGDVSEGDKLPYIAENMLFASAGVK
ADSWELTLAARYTDDIRTQAGQGEIAADNLIKAKTIVDLSGRYFIDDAQELYLTAENLFDEKYVTSRVYGSNFVGKPMTV
SVGYAYKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4772

COG function: function code P; Outer membrane receptor for Fe3+-dicitrate

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790743, Length=229, Percent_Identity=24.8908296943231, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 80339; Mature: 80208

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRNTRSLSLISTALLAALSPQVMAQESDSQPTMEQISIFGKQNPLNTAPGSAHLITQA
CCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEH
ELDNFKYTDIMRTLASIPGVYIQEEDGYGLRPNIGMRGTGQNRSEKITIMEDGVLAAPAP
HCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCC
YASPAAYYFPTPGRMQSVEILKGSSTVKYGPRTTGGVINMLSRQIPEEELAGKLDVAAGQ
CCCCCEEECCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCHHHHCCEEEECCCC
DGFGKLHAYAGGQGERVGAVMEMYRYQADGFKDINGVGGDTGFEKNDALAKVSVRSADDA
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
KYAQVLEFKLKYSDEVSNETYMGLTDSDLETTPYARYSASQKDVMNTEHKQLQINHIIDF
HHHHHHHEEEECCCCCCCCEEECCCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEEEEEC
SDNLTLGTTAYYNDFARNWYKADKVNGLKLSKGGVEAASEFDKDPSMAPMDVRVKANNRS
CCCEEECCHHHHHHHHHHHEHHCCCCCEEECCCCCHHHHHCCCCCCCCCEEEEEEECCHH
YISQGVQTELNWYLANHDLAFGARYHEDEMDRFQWADHYTLDSNQVMTLSESGIPGTDSN
HHHCCCCEEEEEEEECCCEEECCCCCHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCC
RIDSAKAIALYVQDKIQFGDFNITAGLRYEDVTTNRTDWGKENPGRDGQPNKDIDNSFSA
CCCCCEEEEEEEEEEEEECCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TLPSLSATYQINSELLVLAGVQKGFAPASPGNADKKEEQSWNYEAGARYNSGEFSSEAIF
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEE
FYSDYSNMHGECTASKGCDDDNIGDQYNGGEVDVKGLELSLGYSFNTQTDFSFPVKLAYT
EEECCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCEEEEEEE
YTDAQFANSFESDFDPWGDVSEGDKLPYIAENMLFASAGVKADSWELTLAARYTDDIRTQ
ECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCEEEEEEEECCHHHHHC
AGQGEIAADNLIKAKTIVDLSGRYFIDDAQELYLTAENLFDEKYVTSRVYGSNFVGKPMT
CCCCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEHHHHHCHHHHHHHHCCCCCCCCCEE
VSVGYAYKF
EEECEEECC
>Mature Secondary Structure 
SHRNTRSLSLISTALLAALSPQVMAQESDSQPTMEQISIFGKQNPLNTAPGSAHLITQA
CCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEH
ELDNFKYTDIMRTLASIPGVYIQEEDGYGLRPNIGMRGTGQNRSEKITIMEDGVLAAPAP
HCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCC
YASPAAYYFPTPGRMQSVEILKGSSTVKYGPRTTGGVINMLSRQIPEEELAGKLDVAAGQ
CCCCCEEECCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCHHHHCCEEEECCCC
DGFGKLHAYAGGQGERVGAVMEMYRYQADGFKDINGVGGDTGFEKNDALAKVSVRSADDA
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
KYAQVLEFKLKYSDEVSNETYMGLTDSDLETTPYARYSASQKDVMNTEHKQLQINHIIDF
HHHHHHHEEEECCCCCCCCEEECCCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEEEEEC
SDNLTLGTTAYYNDFARNWYKADKVNGLKLSKGGVEAASEFDKDPSMAPMDVRVKANNRS
CCCEEECCHHHHHHHHHHHEHHCCCCCEEECCCCCHHHHHCCCCCCCCCEEEEEEECCHH
YISQGVQTELNWYLANHDLAFGARYHEDEMDRFQWADHYTLDSNQVMTLSESGIPGTDSN
HHHCCCCEEEEEEEECCCEEECCCCCHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCC
RIDSAKAIALYVQDKIQFGDFNITAGLRYEDVTTNRTDWGKENPGRDGQPNKDIDNSFSA
CCCCCEEEEEEEEEEEEECCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TLPSLSATYQINSELLVLAGVQKGFAPASPGNADKKEEQSWNYEAGARYNSGEFSSEAIF
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEE
FYSDYSNMHGECTASKGCDDDNIGDQYNGGEVDVKGLELSLGYSFNTQTDFSFPVKLAYT
EEECCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCCCEEEEEEE
YTDAQFANSFESDFDPWGDVSEGDKLPYIAENMLFASAGVKADSWELTLAARYTDDIRTQ
ECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCEEEEEEEECCHHHHHC
AGQGEIAADNLIKAKTIVDLSGRYFIDDAQELYLTAENLFDEKYVTSRVYGSNFVGKPMT
CCCCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEHHHHHCHHHHHHHHCCCCCCCCCEE
VSVGYAYKF
EEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA