Click here to switch to the map view.

The map label for this gene is rluF [H]

Identifier: 157960503

GI number: 157960503

Start: 835795

End: 836511

Strand: Reverse

Name: rluF [H]

Synonym: Spea_0675

Alternate gene names: 157960503

Gene position: 836511-835795 (Counterclockwise)

Preceding gene: 157960504

Following gene: 157960497

Centisome position: 16.17

GC content: 48.95

Gene sequence:

>717_bases
ATGAGACTCGCAAAATACCTTGCCTTAACCGGCTGCTGTTCACGTCGCGCCGCGACCCGCTTAATCAGAGACGGCTTAGT
TCGTATCGATGAGCGCCTCGCTAACCACGTCGATAGCGTCACCCTAATCGACACGCCAATAGGTCAGCGCAGCATCGAGC
AGATCTATGTTAACGATGAACCGATTCGTGTCGTCGAAGCCAAAGCCTACTGGCTATTTAATAAAGCCGTCGGCACAGAT
AGTCGTCTGCTAGCGCACCTGCCAAACAGCTTATTACACCTGTTGCCGGAGTCTCCACGGCTCTACCCTGTCGGTCGTCT
CGATAAAGACAGCCGCGGCTTACTGATCTTAACCAACGATGGCGAATTAACTCATAAGCTAATGCACCCTGATTTTGGTC
ATGCTAAGACTTACCATGTGCAAGTTGACCGCCCTTTCGATGATGCTTTTCTTGTAAACATGGCCGCTGGGGTGAGTTAT
AAAGATGTCGTCACTTTACCTTGTATCATGCAGCGTTTAGCTGCTGATAAATATGAGATTACTTTGACTCAGGGGCTAAA
TCGTCAGATACGCCGCATGTCACAGGCGTTGGGGTTTAAGGTTATCGACCTTAAACGGGTATCGATTCAGAGTTTACAGT
TAGGGGATTTGCCTGAAGGGTCTATGCGACCTCTGAGTTCAGCTGAATTGGCTGACTTGTTGGATTCTGTTGCTTAG

Upstream 100 bases:

>100_bases
CTGGTGTCTTGCCCACGCATGGAGCATACGCCAAATGGCCATCAATGATTACCGTGAACTCACACAGAAATATTTAGCTA
AAGAATAACAGCCACAATTA

Downstream 100 bases:

>100_bases
GTTCTAGGTTAGAGTTAACCATTAAACTTGTTCTTACCAAGCTGCGCTTGATAGTTCCAACTAACCTACTTTGGACAAAA
GCAACTCACTACGGCGAAGC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLAKYLALTGCCSRRAATRLIRDGLVRIDERLANHVDSVTLIDTPIGQRSIEQIYVNDEPIRVVEAKAYWLFNKAVGTD
SRLLAHLPNSLLHLLPESPRLYPVGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQVDRPFDDAFLVNMAAGVSY
KDVVTLPCIMQRLAADKYEITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLPEGSMRPLSSAELADLLDSVA

Sequences:

>Translated_238_residues
MRLAKYLALTGCCSRRAATRLIRDGLVRIDERLANHVDSVTLIDTPIGQRSIEQIYVNDEPIRVVEAKAYWLFNKAVGTD
SRLLAHLPNSLLHLLPESPRLYPVGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQVDRPFDDAFLVNMAAGVSY
KDVVTLPCIMQRLAADKYEITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLPEGSMRPLSSAELADLLDSVA
>Mature_238_residues
MRLAKYLALTGCCSRRAATRLIRDGLVRIDERLANHVDSVTLIDTPIGQRSIEQIYVNDEPIRVVEAKAYWLFNKAVGTD
SRLLAHLPNSLLHLLPESPRLYPVGRLDKDSRGLLILTNDGELTHKLMHPDFGHAKTYHVQVDRPFDDAFLVNMAAGVSY
KDVVTLPCIMQRLAADKYEITLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLPEGSMRPLSSAELADLLDSVA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=38.135593220339, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=27.6150627615063, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787524, Length=225, Percent_Identity=32.4444444444444, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081838, Length=188, Percent_Identity=31.9148936170213, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26654; Mature: 26654

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAKYLALTGCCSRRAATRLIRDGLVRIDERLANHVDSVTLIDTPIGQRSIEQIYVNDE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCC
PIRVVEAKAYWLFNKAVGTDSRLLAHLPNSLLHLLPESPRLYPVGRLDKDSRGLLILTND
CEEEEEEEEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCCCEEEEECC
GELTHKLMHPDFGHAKTYHVQVDRPFDDAFLVNMAAGVSYKDVVTLPCIMQRLAADKYEI
CHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEE
TLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLPEGSMRPLSSAELADLLDSVA
EEHHHHHHHHHHHHHHHCEEEEEEEEHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRLAKYLALTGCCSRRAATRLIRDGLVRIDERLANHVDSVTLIDTPIGQRSIEQIYVNDE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCC
PIRVVEAKAYWLFNKAVGTDSRLLAHLPNSLLHLLPESPRLYPVGRLDKDSRGLLILTND
CEEEEEEEEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCCCEEEEECC
GELTHKLMHPDFGHAKTYHVQVDRPFDDAFLVNMAAGVSYKDVVTLPCIMQRLAADKYEI
CHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEE
TLTQGLNRQIRRMSQALGFKVIDLKRVSIQSLQLGDLPEGSMRPLSSAELADLLDSVA
EEHHHHHHHHHHHHHHHCEEEEEEEEHHHHEEECCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]