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The map label for this gene is pfkA [H]

Identifier: 157373039

GI number: 157373039

Start: 5326185

End: 5327147

Strand: Reverse

Name: pfkA [H]

Synonym: Spro_4807

Alternate gene names: 157373039

Gene position: 5327147-5326185 (Counterclockwise)

Preceding gene: 157373040

Following gene: 157373038

Centisome position: 97.77

GC content: 53.89

Gene sequence:

>963_bases
ATGATCAAGAAAATCGGTGTACTGACAAGTGGCGGTGATTCGCCGGGTATGAACGCTGCGATCCGTGGCGTTGTGCGTGC
TGCACTTTCAGAAGGTCTGGAAGTTTTTGGTATTTACGATGGCTACCTTGGCTTGTACGAAGATCGCATGGAAAAATTGG
ACCGCTACAGCGTGTCCGATATGATCAATCGCGGCGGCACCTTCCTTGGTTCAGCCCGTTTCCCGGAGTTCAGAGACGAC
AGCGTACGTGCCAAGGCGATTGAAAATCTGCAAAACCGCGGCATTGATGCGCTGGTGGTGATTGGCGGTGACGGTTCCTA
TATGGGGGCTAAACGCCTGACGGAAGAAGGTTTCCCTTGTATTGGCTTGCCGGGCACTATCGATAACGACGTGGCCGGTA
CCGACTACACCATCGGTTTCTTCACCGCGCTTGAAACCGTGGTGGAAGCGATTGACCGCTTGCGCGATACCTCATCTTCA
CATCAGCGTATTTCTATCGTTGAAGTGATGGGGCGTTATTGCGGCGATCTGACGCTGGCGGCGGCGATTGCCGGCGGTTG
CGAGTTCATCGTGTTGCCTGAAATTGAGTTCAACCGCGAAGATCTGGTTTGTGAAATCAAAGCCGGCATCGACAAGGGTA
AAAAACACGCGATTGTGGCAATCACCGAGCATATCTGCGATATCGATGAGCTGGCGCGCCATATCGAGCAAGAAACCAAG
CGTGAAACCCGCGCTACCGTGTTGGGGCATATTCAGCGCGGTGGTTCACCGGTAGCCTACGACCGTATTCTGGCTTCCCG
CATGGGTGCTTACGCCATCGAGCTGTTGTTGCAGGGCTACGGCGGCCGCTGTGTCGGTATCCAAAACGAGAAGATGGTGC
ACCACGACATCATCGATGCGATCGAAAACATGAAGCGTCCATTCAAGGGCGACTGGCTGGAAACGGCGAAAAAACTGTAC
TGA

Upstream 100 bases:

>100_bases
CAATAAGCCGTAAAGCTGATTTTCTACAGCCGGAGAGGTTCCAGGACAATCGGCGAATACAGAATTCTTAAGAAATTGCA
TCTACAGTTCAGAGGTAGTC

Downstream 100 bases:

>100_bases
TCCCGTTTTAATTCAGTCAACAGAAGCCTCCGCATGCGGAGGCTTTTTTATGCCTGCTGCGGTTATATTCCAGAATCGTC
TAGCCCAAAATTTTTTATTC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAALSEGLEVFGIYDGYLGLYEDRMEKLDRYSVSDMINRGGTFLGSARFPEFRDD
SVRAKAIENLQNRGIDALVVIGGDGSYMGAKRLTEEGFPCIGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIEFNREDLVCEIKAGIDKGKKHAIVAITEHICDIDELARHIEQETK
RETRATVLGHIQRGGSPVAYDRILASRMGAYAIELLLQGYGGRCVGIQNEKMVHHDIIDAIENMKRPFKGDWLETAKKLY

Sequences:

>Translated_320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAALSEGLEVFGIYDGYLGLYEDRMEKLDRYSVSDMINRGGTFLGSARFPEFRDD
SVRAKAIENLQNRGIDALVVIGGDGSYMGAKRLTEEGFPCIGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIEFNREDLVCEIKAGIDKGKKHAIVAITEHICDIDELARHIEQETK
RETRATVLGHIQRGGSPVAYDRILASRMGAYAIELLLQGYGGRCVGIQNEKMVHHDIIDAIENMKRPFKGDWLETAKKLY
>Mature_320_residues
MIKKIGVLTSGGDSPGMNAAIRGVVRAALSEGLEVFGIYDGYLGLYEDRMEKLDRYSVSDMINRGGTFLGSARFPEFRDD
SVRAKAIENLQNRGIDALVVIGGDGSYMGAKRLTEEGFPCIGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIEFNREDLVCEIKAGIDKGKKHAIVAITEHICDIDELARHIEQETK
RETRATVLGHIQRGGSPVAYDRILASRMGAYAIELLLQGYGGRCVGIQNEKMVHHDIIDAIENMKRPFKGDWLETAKKLY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=41.3461538461538, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=40.4458598726115, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=40.4458598726115, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=40.4458598726115, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=40.4458598726115, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=41.4790996784566, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=88.4375, Blast_Score=584, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=39.871382636656, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=37.987012987013, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=31.4878892733564, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=38.0116959064327, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=343, Percent_Identity=34.1107871720117, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=42.3076923076923, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=41.8006430868167, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=41.6666666666667, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35068; Mature: 35068

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGVLTSGGDSPGMNAAIRGVVRAALSEGLEVFGIYDGYLGLYEDRMEKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDDSVRAKAIENLQNRGIDALVVIGGDGSYMGAKRLTEEGFPC
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCE
IGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAIAGGCEFIVLPEIEFNREDLVCEIKAGIDKGKKHAIVAITEHICDIDELARHIEQETK
HHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHH
RETRATVLGHIQRGGSPVAYDRILASRMGAYAIELLLQGYGGRCVGIQNEKMVHHDIIDA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IENMKRPFKGDWLETAKKLY
HHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MIKKIGVLTSGGDSPGMNAAIRGVVRAALSEGLEVFGIYDGYLGLYEDRMEKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDDSVRAKAIENLQNRGIDALVVIGGDGSYMGAKRLTEEGFPC
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCE
IGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAIAGGCEFIVLPEIEFNREDLVCEIKAGIDKGKKHAIVAITEHICDIDELARHIEQETK
HHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHH
RETRATVLGHIQRGGSPVAYDRILASRMGAYAIELLLQGYGGRCVGIQNEKMVHHDIIDA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IENMKRPFKGDWLETAKKLY
HHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA