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The map label for this gene is sbp [H]

Identifier: 157373038

GI number: 157373038

Start: 5325003

End: 5325998

Strand: Reverse

Name: sbp [H]

Synonym: Spro_4806

Alternate gene names: 157373038

Gene position: 5325998-5325003 (Counterclockwise)

Preceding gene: 157373039

Following gene: 157373035

Centisome position: 97.75

GC content: 54.62

Gene sequence:

>996_bases
ATGCGTATGCGTAAATGGGGTGCAGGTCTGACAATAATGCTGCTGGCGTCCGGCGCCATGGCGAAAGATATCCAATTGCT
GAACGTTTCATACGACCCGACGCGTGAGTTCTATCAGGAATACAACACCGCATTCGGTAAATACTGGCAGCAGCAGACCG
GCGATAAAGTTACGGTGCGCCAGTCGCATGGCGGCTCCGGCAAGCAGGCGACTTCGGTGATTAACGGCATTGAGGCCGAC
GTGGTGACACTGGCACTGGCCTATGACGTGGACGCTATCGCTGAGCGCGGGCGCATTGATAAAGAGTGGATCAAACGTCT
GCCGGACAACTCGGCACCTTATACCTCGACCATTGTGTTCCTGGTGCGCAAAGGTAATCCAAAGCAAATTCACGATTGGG
CGGATTTGATCAAACCGGGCGTCTCGGTAATCACCCCGAACCCGAAAACTTCCGGTGGCGCACGCTGGAACTATCTGGCA
GCCTGGGGTTATGCACTGCATCAGAACAATAACGATCAGGCCAAGGCGCAAGAATTCGTTAAAAACCTGTATAAGAACGT
CGAAGTGCTGGATTCCGGTGCGCGCGGTTCAACTAATACCTTCGTTGAACGCGGTATCGGTGATGTGCTGATCGCCTGGG
AGAACGAAGCGCTGCTGGCGGAAAAAGAGCTGGGCAAGGACAAGTTTGAGATTATCACCCCAAGCGAATCGATTCTGGCC
GAGCCGACCGTGTCGGTGGTGGATAAAGTGGTTGATAAGCGCGGTACCCGTGATGTGGCTACGGCTTACCTGAAGTATCT
GTATACGCCGGAAGGGCAGACCATCGCGGCGAAAAACTATTACCGTCCACGCGATGCGGCGGTAGCGGCCAAGTTTGCCG
ACCAGTTCCCGAAACTGAAACTGTTTACCGTGGATGATACTTTCGGCGGCTGGACCCAGGCGCAGAAGGTGCACTTTGCC
ACCGGCGGCGTGTTTGACGAAATCAGCAAACGTTGA

Upstream 100 bases:

>100_bases
AAAATTTTTTATTCTTTAATTGATAGCCAAGTTTCTGGCAGGCTCTGATGCTAAATCAACCGATTGAGGTTGCGATTATT
TTTCCTGGGGGAGCGAGTCG

Downstream 100 bases:

>100_bases
TGCTCAATGGGCACCAATAAAAAACCCCGGCAAGCCGGGGTTTTTTATTTATAAGCCGGGAGAAATCAGGCTTTTTTAGC
AGCAGCAGCGGCTTTGACGA

Product: sulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRMRKWGAGLTIMLLASGAMAKDIQLLNVSYDPTREFYQEYNTAFGKYWQQQTGDKVTVRQSHGGSGKQATSVINGIEAD
VVTLALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVFLVRKGNPKQIHDWADLIKPGVSVITPNPKTSGGARWNYLA
AWGYALHQNNNDQAKAQEFVKNLYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAEKELGKDKFEIITPSESILA
EPTVSVVDKVVDKRGTRDVATAYLKYLYTPEGQTIAAKNYYRPRDAAVAAKFADQFPKLKLFTVDDTFGGWTQAQKVHFA
TGGVFDEISKR

Sequences:

>Translated_331_residues
MRMRKWGAGLTIMLLASGAMAKDIQLLNVSYDPTREFYQEYNTAFGKYWQQQTGDKVTVRQSHGGSGKQATSVINGIEAD
VVTLALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVFLVRKGNPKQIHDWADLIKPGVSVITPNPKTSGGARWNYLA
AWGYALHQNNNDQAKAQEFVKNLYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAEKELGKDKFEIITPSESILA
EPTVSVVDKVVDKRGTRDVATAYLKYLYTPEGQTIAAKNYYRPRDAAVAAKFADQFPKLKLFTVDDTFGGWTQAQKVHFA
TGGVFDEISKR
>Mature_331_residues
MRMRKWGAGLTIMLLASGAMAKDIQLLNVSYDPTREFYQEYNTAFGKYWQQQTGDKVTVRQSHGGSGKQATSVINGIEAD
VVTLALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVFLVRKGNPKQIHDWADLIKPGVSVITPNPKTSGGARWNYLA
AWGYALHQNNNDQAKAQEFVKNLYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAEKELGKDKFEIITPSESILA
EPTVSVVDKVVDKRGTRDVATAYLKYLYTPEGQTIAAKNYYRPRDAAVAAKFADQFPKLKLFTVDDTFGGWTQAQKVHFA
TGGVFDEISKR

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=329, Percent_Identity=85.1063829787234, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1788765, Length=306, Percent_Identity=47.7124183006536, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36809; Mature: 36809

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRKWGAGLTIMLLASGAMAKDIQLLNVSYDPTREFYQEYNTAFGKYWQQQTGDKVTVR
CCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
QSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVF
ECCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEE
LVRKGNPKQIHDWADLIKPGVSVITPNPKTSGGARWNYLAAWGYALHQNNNDQAKAQEFV
EEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCEEHHEEHEEHEECCCCCHHHHHHHH
KNLYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAEKELGKDKFEIITPSESILA
HHHHCCHHEECCCCCCCCHHHHHCCCCCEEEEECCCCCHHHHHCCCCCEEEECCCHHHHC
EPTVSVVDKVVDKRGTRDVATAYLKYLYTPEGQTIAAKNYYRPRDAAVAAKFADQFPKLK
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEE
LFTVDDTFGGWTQAQKVHFATGGVFDEISKR
EEEECCCCCCCCHHCEEEEECCCHHHHHHCC
>Mature Secondary Structure
MRMRKWGAGLTIMLLASGAMAKDIQLLNVSYDPTREFYQEYNTAFGKYWQQQTGDKVTVR
CCCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
QSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVF
ECCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEE
LVRKGNPKQIHDWADLIKPGVSVITPNPKTSGGARWNYLAAWGYALHQNNNDQAKAQEFV
EEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCEEHHEEHEEHEECCCCCHHHHHHHH
KNLYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLAEKELGKDKFEIITPSESILA
HHHHCCHHEECCCCCCCCHHHHHCCCCCEEEEECCCCCHHHHHCCCCCEEEECCCHHHHC
EPTVSVVDKVVDKRGTRDVATAYLKYLYTPEGQTIAAKNYYRPRDAAVAAKFADQFPKLK
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEE
LFTVDDTFGGWTQAQKVHFATGGVFDEISKR
EEEECCCCCCCCHHCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]