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The map label for this gene is lpdA [H]

Identifier: 157373009

GI number: 157373009

Start: 5291251

End: 5292699

Strand: Direct

Name: lpdA [H]

Synonym: Spro_4777

Alternate gene names: 157373009

Gene position: 5291251-5292699 (Clockwise)

Preceding gene: 157373008

Following gene: 157373014

Centisome position: 97.11

GC content: 59.14

Gene sequence:

>1449_bases
ATGAAACAGTTGAATGTTGACGTCGCCGTTATCGGTGGCGGCACCGCCGGTCTGGGCGCCTATCGCGCCGCCAAGCTTTC
GACTCCCAGCGTAGTCATGATTGAGGGCGGGGCCTACGGCACCACCTGTGCCCGCGTTGGCTGCATGCCATCAAAATTGC
TGATTGCTGCCGCCGAGGCGGTGCATCAAATCGAGCGTGCGCCAGGCTTTGGCGTATATCCCGCCGGTAAAACCACCATT
AACGGTCGTGAAGTGATGGACCGCGTCAAACGCGAACGCGACCGTTTCGTCGGCTTTGTATTGGAAGGTGTCGACGAGAT
CCCGGCGGCCGACAAAATTAGCGGTTACGCCCGTTTTATCGACGACAACACCCTGCAGGTGGATGAACATACGCGCATCG
TGGCACAGCGCATTGTTATCGCCACCGGTTCCCGCCCAAGCTGGCCCGCGCCCTGGAATACACTGGGTGACCGACTGATC
GTCAATGACGACGTCTTCAACTGGGATGATTTACCGCAGTCGGTCGCGGTATTCGGGCCAGGTGTGATTGGCCTGGAACT
GGGTCAGGCGCTGCATCGTCTCGGGGTTGAGACCAAAGTGTTTGGCGTTGGCGGCGCCGTCGGCCCGTTGACTGACAGCA
CGGTACGCAACTACGCCGCTAAAACCCTGGGCGAAGAGTTCTATCTTGATGCCGATGTGAAAGTGGAGATGATGCAGCGC
GAAGGCGACAAAGTGTTTATCCGTTATCAGGATTTACACGGCAAGACGCAGGAGATCATGGTGGATTATGTGTTGGCCGC
CACCGGTCGCCGACCGAACGTCGATAATCTGGGCCTGGAGAATACCGGCCTGCTACTCGACGTTCGGGGCGTACCCCAGG
CCGATCGCCTGACGATGCAAACCAGCGTGCCACATATCTTTATTGCCGGTGACGCCAGCAATCAACTGCCGCTGCTGCAC
GAAGCCAGCGATCAGGCGCGCATTGCCGGTAGCAACGCCGGCAGCTACCCGGAAGTCGCGCCGGGCCTGCGACGCAGCGC
GATCTCGGTGGTATTCTCCGATCCGCAGATTGCCATGGTCGGTTCCACCTTCCGTGAACTGAGCGAGAAATTCAGCGCCT
GTGGCTGTTTTGAAATCGGTGAGGTGTCATTCGAGAACCAGGGGCGTTCACGCGTGATGCTACGTAACAAAGGTATCCTG
CACGTCTACGGCGAACAGGGCACCGGACGTTTCCTCGGTGCAGAGATGATGGGGCCGGAGGTTGAGCATATCGCCCATCT
GCTGGCCTGGGCGCATCAGCAGCAAATGACCATCAATCAGATGCTGGACATGCCGTTTTATCATCCGGTGATCGAAGAAG
GTCTGCGCACCGCGCTGCGCGATCTGCAGGCCAAGCTTAAACTGGGTGAGGCCGAGGCGGAACGCTGCCAGCGCTGCCCG
GGCGAGTAA

Upstream 100 bases:

>100_bases
TGGAAAGCTTCCTGTCAGCCTGATTTATAAGTAATAACAAAGCCAACGTGAACTTTTGGCGGGCTCCGGCCCGCCCTTTT
TTTAGCTTTCAGGAGCGGAT

Downstream 100 bases:

>100_bases
TCTTTCTAAAAACAAAGGGCGCAGTTCTGCCTATGAACTGCGCCCTTGATACTGAGTAACCTAATTAAGCCAAGCGTTGT
TTCGCTGCGGCGATCGCGCT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKQLNVDVAVIGGGTAGLGAYRAAKLSTPSVVMIEGGAYGTTCARVGCMPSKLLIAAAEAVHQIERAPGFGVYPAGKTTI
NGREVMDRVKRERDRFVGFVLEGVDEIPAADKISGYARFIDDNTLQVDEHTRIVAQRIVIATGSRPSWPAPWNTLGDRLI
VNDDVFNWDDLPQSVAVFGPGVIGLELGQALHRLGVETKVFGVGGAVGPLTDSTVRNYAAKTLGEEFYLDADVKVEMMQR
EGDKVFIRYQDLHGKTQEIMVDYVLAATGRRPNVDNLGLENTGLLLDVRGVPQADRLTMQTSVPHIFIAGDASNQLPLLH
EASDQARIAGSNAGSYPEVAPGLRRSAISVVFSDPQIAMVGSTFRELSEKFSACGCFEIGEVSFENQGRSRVMLRNKGIL
HVYGEQGTGRFLGAEMMGPEVEHIAHLLAWAHQQQMTINQMLDMPFYHPVIEEGLRTALRDLQAKLKLGEAEAERCQRCP
GE

Sequences:

>Translated_482_residues
MKQLNVDVAVIGGGTAGLGAYRAAKLSTPSVVMIEGGAYGTTCARVGCMPSKLLIAAAEAVHQIERAPGFGVYPAGKTTI
NGREVMDRVKRERDRFVGFVLEGVDEIPAADKISGYARFIDDNTLQVDEHTRIVAQRIVIATGSRPSWPAPWNTLGDRLI
VNDDVFNWDDLPQSVAVFGPGVIGLELGQALHRLGVETKVFGVGGAVGPLTDSTVRNYAAKTLGEEFYLDADVKVEMMQR
EGDKVFIRYQDLHGKTQEIMVDYVLAATGRRPNVDNLGLENTGLLLDVRGVPQADRLTMQTSVPHIFIAGDASNQLPLLH
EASDQARIAGSNAGSYPEVAPGLRRSAISVVFSDPQIAMVGSTFRELSEKFSACGCFEIGEVSFENQGRSRVMLRNKGIL
HVYGEQGTGRFLGAEMMGPEVEHIAHLLAWAHQQQMTINQMLDMPFYHPVIEEGLRTALRDLQAKLKLGEAEAERCQRCP
GE
>Mature_482_residues
MKQLNVDVAVIGGGTAGLGAYRAAKLSTPSVVMIEGGAYGTTCARVGCMPSKLLIAAAEAVHQIERAPGFGVYPAGKTTI
NGREVMDRVKRERDRFVGFVLEGVDEIPAADKISGYARFIDDNTLQVDEHTRIVAQRIVIATGSRPSWPAPWNTLGDRLI
VNDDVFNWDDLPQSVAVFGPGVIGLELGQALHRLGVETKVFGVGGAVGPLTDSTVRNYAAKTLGEEFYLDADVKVEMMQR
EGDKVFIRYQDLHGKTQEIMVDYVLAATGRRPNVDNLGLENTGLLLDVRGVPQADRLTMQTSVPHIFIAGDASNQLPLLH
EASDQARIAGSNAGSYPEVAPGLRRSAISVVFSDPQIAMVGSTFRELSEKFSACGCFEIGEVSFENQGRSRVMLRNKGIL
HVYGEQGTGRFLGAEMMGPEVEHIAHLLAWAHQQQMTINQMLDMPFYHPVIEEGLRTALRDLQAKLKLGEAEAERCQRCP
GE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=27.8723404255319, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI50301238, Length=449, Percent_Identity=25.1670378619154, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI22035672, Length=297, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1786307, Length=430, Percent_Identity=27.2093023255814, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=25.3191489361702, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789915, Length=351, Percent_Identity=29.9145299145299, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=23.3115468409586, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=28.6315789473684, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983429, Length=366, Percent_Identity=26.775956284153, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983419, Length=366, Percent_Identity=26.775956284153, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557007, Length=328, Percent_Identity=26.219512195122, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=27.5, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325166, Length=386, Percent_Identity=30.3108808290155, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325240, Length=491, Percent_Identity=23.0142566191446, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=27.4468085106383, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=27.6073619631902, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=27.6073619631902, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640551, Length=482, Percent_Identity=27.3858921161826, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=23.8775510204082, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52432; Mature: 52432

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLNVDVAVIGGGTAGLGAYRAAKLSTPSVVMIEGGAYGTTCARVGCMPSKLLIAAAEA
CCCEEEEEEEECCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIERAPGFGVYPAGKTTINGREVMDRVKRERDRFVGFVLEGVDEIPAADKISGYARFI
HHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHCCCCEEEE
DDNTLQVDEHTRIVAQRIVIATGSRPSWPAPWNTLGDRLIVNDDVFNWDDLPQSVAVFGP
CCCEEEECHHHHHHHHEEEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCHHHHHCC
GVIGLELGQALHRLGVETKVFGVGGAVGPLTDSTVRNYAAKTLGEEFYLDADVKVEMMQR
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECC
EGDKVFIRYQDLHGKTQEIMVDYVLAATGRRPNVDNLGLENTGLLLDVRGVPQADRLTMQ
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHCEEEE
TSVPHIFIAGDASNQLPLLHEASDQARIAGSNAGSYPEVAPGLRRSAISVVFSDPQIAMV
ECCCEEEEECCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCHHHCEEEEEECCCCEEEH
GSTFRELSEKFSACGCFEIGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAEMMGPE
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEECHHHCCCC
VEHIAHLLAWAHQQQMTINQMLDMPFYHPVIEEGLRTALRDLQAKLKLGEAEAERCQRCP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
GE
CC
>Mature Secondary Structure
MKQLNVDVAVIGGGTAGLGAYRAAKLSTPSVVMIEGGAYGTTCARVGCMPSKLLIAAAEA
CCCEEEEEEEECCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIERAPGFGVYPAGKTTINGREVMDRVKRERDRFVGFVLEGVDEIPAADKISGYARFI
HHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHCCCCEEEE
DDNTLQVDEHTRIVAQRIVIATGSRPSWPAPWNTLGDRLIVNDDVFNWDDLPQSVAVFGP
CCCEEEECHHHHHHHHEEEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCHHHHHCC
GVIGLELGQALHRLGVETKVFGVGGAVGPLTDSTVRNYAAKTLGEEFYLDADVKVEMMQR
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECC
EGDKVFIRYQDLHGKTQEIMVDYVLAATGRRPNVDNLGLENTGLLLDVRGVPQADRLTMQ
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHCEEEE
TSVPHIFIAGDASNQLPLLHEASDQARIAGSNAGSYPEVAPGLRRSAISVVFSDPQIAMV
ECCCEEEEECCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCHHHCEEEEEECCCCEEEH
GSTFRELSEKFSACGCFEIGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAEMMGPE
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEECHHHCCCC
VEHIAHLLAWAHQQQMTINQMLDMPFYHPVIEEGLRTALRDLQAKLKLGEAEAERCQRCP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]