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The map label for this gene is rsmB

Identifier: 157372746

GI number: 157372746

Start: 5005330

End: 5006619

Strand: Direct

Name: rsmB

Synonym: Spro_4513

Alternate gene names: 157372746

Gene position: 5005330-5006619 (Clockwise)

Preceding gene: 157372745

Following gene: 157372747

Centisome position: 91.86

GC content: 55.58

Gene sequence:

>1290_bases
ATGAAAAACAATTACAATCTCCGCAGCATCGCAGCCATTGCCATCGGTCAGGTGTTGGATCAGGGCCAGTCACTCAGTAC
TGTCCTGCCGTCACTGCAAAAAACCATTTCTGACAAGGATCGTGCTCTGCTGCAGGAACTGTGCTTTGGCACCCTGCGCG
TCCTGCCACAGTTGGAATGGTGTATTCAACAATTAATGGCCAAGCCAATGACTGGCAAGCAGCGCACGCTGCACTATTTG
TTGATGGTCGGCTTATACCAACTGCTGTATACCCGTATCCCCGCTCATGCGGTGCTGGCGGAAACCGTAGAAGGCGCAGT
AGCGCTGAAACGCCCGCAGCTTAAGGGCCTGATCAACGGCGTATTGCGCCAGTTCCAACGCCAGCAAGAAGAATTACTGC
AACGCGCTGCCAATAATGACAGCCGCTATCTGCACCCGAGCTGGCTGTTGAAACGTATCCAGCAGGCCTATCCCGCACAG
TGGGAGCAGATCATCGATGCCAATAATCAGAAGCCGCCAATGTGGCTGCGCGTCAACCGTTTGCATCATACTCGCGAAGA
CTACTTGCAGTTGATGGAACAGGCGGGCATTGCCGCGGAACCTCATGCCGAATATCGCGATGCGGTACGTTTGCTGGCAC
CCTGCGCCGTTACCGACCTGCCAGGCTTTGCCGACGGCTGGGTCACCGTGCAGGATGCCTCCGCGCAGGGCTGTGTCGAT
CTGCTGGATCCGCAAGACGGGGAACAGATCCTCGATTTGTGTGCGGCTCCCGGTGGAAAAACTACCCATATTTTAGAGGC
GGCTCCCAAAGCCCATGTGATGGCCGTCGATATCGATGAACAGCGCCTGAAGCGGGTCAAAGAAAACCTGCAACGTCTGC
GTTTACACGCCGAAGTAAAGCAAGGCGATGGCCGCACTCCGCAACAATGGTGCGGCGACAAGCAATTCGATCGCATTTTG
CTGGATGCTCCTTGCTCTGCCACCGGTGTGATCCGCCGTCACCCAGATATTAAATGGTTACGACGCGACAGTGATATCGC
TGAACTGGCCGCGCTGCAGGCCGAGATTATTGAGGCCATCTGGCCGCATCTCAAAAGCGGAGGAGTGATGGTGTATGCCA
CCTGCTCCATCTTGCCGGCAGAAAATGCTGAGCAGGTTAGCGCCTTCCTCCAGCGTCATGCCGATGCCCGTCTGGTTGAA
ACCGGCAACCAGCAGCAGCCTGGCCGACAGAATCTTCCGCATCCAGAGGATGGGGATGGCTTCTTTTACGCTAAGCTGAT
TAAAATGTAG

Upstream 100 bases:

>100_bases
TGGTTTACGCCGGGCAACCGGTTATAAACCGCCCCCACTGCCCGGCCAATAATCGCCGGGCTGTTTCTTTCTTAATGCCG
ATCGTTATCAGCTTTAGCCT

Downstream 100 bases:

>100_bases
CTGTTCAGTACTCTGGGCGCGGCTGTTGCGCCCTTTCAGATAATCTTCAGCGGTTTCAGTGTGAAGCAGAGAAGCGAATG
AAAATAATTATTCTTGGCGC

Product: 16S rRNA methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKNNYNLRSIAAIAIGQVLDQGQSLSTVLPSLQKTISDKDRALLQELCFGTLRVLPQLEWCIQQLMAKPMTGKQRTLHYL
LMVGLYQLLYTRIPAHAVLAETVEGAVALKRPQLKGLINGVLRQFQRQQEELLQRAANNDSRYLHPSWLLKRIQQAYPAQ
WEQIIDANNQKPPMWLRVNRLHHTREDYLQLMEQAGIAAEPHAEYRDAVRLLAPCAVTDLPGFADGWVTVQDASAQGCVD
LLDPQDGEQILDLCAAPGGKTTHILEAAPKAHVMAVDIDEQRLKRVKENLQRLRLHAEVKQGDGRTPQQWCGDKQFDRIL
LDAPCSATGVIRRHPDIKWLRRDSDIAELAALQAEIIEAIWPHLKSGGVMVYATCSILPAENAEQVSAFLQRHADARLVE
TGNQQQPGRQNLPHPEDGDGFFYAKLIKM

Sequences:

>Translated_429_residues
MKNNYNLRSIAAIAIGQVLDQGQSLSTVLPSLQKTISDKDRALLQELCFGTLRVLPQLEWCIQQLMAKPMTGKQRTLHYL
LMVGLYQLLYTRIPAHAVLAETVEGAVALKRPQLKGLINGVLRQFQRQQEELLQRAANNDSRYLHPSWLLKRIQQAYPAQ
WEQIIDANNQKPPMWLRVNRLHHTREDYLQLMEQAGIAAEPHAEYRDAVRLLAPCAVTDLPGFADGWVTVQDASAQGCVD
LLDPQDGEQILDLCAAPGGKTTHILEAAPKAHVMAVDIDEQRLKRVKENLQRLRLHAEVKQGDGRTPQQWCGDKQFDRIL
LDAPCSATGVIRRHPDIKWLRRDSDIAELAALQAEIIEAIWPHLKSGGVMVYATCSILPAENAEQVSAFLQRHADARLVE
TGNQQQPGRQNLPHPEDGDGFFYAKLIKM
>Mature_429_residues
MKNNYNLRSIAAIAIGQVLDQGQSLSTVLPSLQKTISDKDRALLQELCFGTLRVLPQLEWCIQQLMAKPMTGKQRTLHYL
LMVGLYQLLYTRIPAHAVLAETVEGAVALKRPQLKGLINGVLRQFQRQQEELLQRAANNDSRYLHPSWLLKRIQQAYPAQ
WEQIIDANNQKPPMWLRVNRLHHTREDYLQLMEQAGIAAEPHAEYRDAVRLLAPCAVTDLPGFADGWVTVQDASAQGCVD
LLDPQDGEQILDLCAAPGGKTTHILEAAPKAHVMAVDIDEQRLKRVKENLQRLRLHAEVKQGDGRTPQQWCGDKQFDRIL
LDAPCSATGVIRRHPDIKWLRRDSDIAELAALQAEIIEAIWPHLKSGGVMVYATCSILPAENAEQVSAFLQRHADARLVE
TGNQQQPGRQNLPHPEDGDGFFYAKLIKM

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=345, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI76150623, Length=345, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI32698918, Length=177, Percent_Identity=35.5932203389831, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI11545785, Length=171, Percent_Identity=34.5029239766082, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301336155, Length=214, Percent_Identity=30.3738317757009, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI39995082, Length=214, Percent_Identity=30.3738317757009, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI40316918, Length=175, Percent_Identity=29.1428571428571, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI270288818, Length=293, Percent_Identity=27.3037542662116, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI8922322, Length=293, Percent_Identity=27.3037542662116, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI270288816, Length=293, Percent_Identity=27.3037542662116, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI23199998, Length=293, Percent_Identity=27.3037542662116, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI2367212, Length=428, Percent_Identity=73.1308411214953, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI87081985, Length=310, Percent_Identity=27.741935483871, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=315, Percent_Identity=29.5238095238095, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25143471, Length=296, Percent_Identity=30.0675675675676, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534021, Length=214, Percent_Identity=28.9719626168224, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71998419, Length=214, Percent_Identity=28.9719626168224, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=344, Percent_Identity=30.2325581395349, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319447, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22024126, Length=313, Percent_Identity=29.073482428115, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24668781, Length=236, Percent_Identity=29.2372881355932, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355201, Length=186, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356513, Length=228, Percent_Identity=29.8245614035088, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920494, Length=163, Percent_Identity=30.6748466257669, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMB_SERP5 (A8GKG7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480735.1
- ProteinModelPortal:   A8GKG7
- SMR:   A8GKG7
- STRING:   A8GKG7
- GeneID:   5603907
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4513
- eggNOG:   COG0144
- HOGENOM:   HBG726909
- OMA:   ANNAHPP
- ProtClustDB:   PRK10901
- BioCyc:   SPRO399741:SPRO_4513-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01856
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR00563

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: =2.1.1.176

Molecular weight: Translated: 48280; Mature: 48280

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 375-375 BINDING 277-277 BINDING 303-303 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNYNLRSIAAIAIGQVLDQGQSLSTVLPSLQKTISDKDRALLQELCFGTLRVLPQLEW
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
CIQQLMAKPMTGKQRTLHYLLMVGLYQLLYTRIPAHAVLAETVEGAVALKRPQLKGLING
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
VLRQFQRQQEELLQRAANNDSRYLHPSWLLKRIQQAYPAQWEQIIDANNQKPPMWLRVNR
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEHHH
LHHTREDYLQLMEQAGIAAEPHAEYRDAVRLLAPCAVTDLPGFADGWVTVQDASAQGCVD
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHH
LLDPQDGEQILDLCAAPGGKTTHILEAAPKAHVMAVDIDEQRLKRVKENLQRLRLHAEVK
CCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
QGDGRTPQQWCGDKQFDRILLDAPCSATGVIRRHPDIKWLRRDSDIAELAALQAEIIEAI
CCCCCCHHHHCCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHH
WPHLKSGGVMVYATCSILPAENAEQVSAFLQRHADARLVETGNQQQPGRQNLPHPEDGDG
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCC
FFYAKLIKM
EEHHEECCC
>Mature Secondary Structure
MKNNYNLRSIAAIAIGQVLDQGQSLSTVLPSLQKTISDKDRALLQELCFGTLRVLPQLEW
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
CIQQLMAKPMTGKQRTLHYLLMVGLYQLLYTRIPAHAVLAETVEGAVALKRPQLKGLING
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
VLRQFQRQQEELLQRAANNDSRYLHPSWLLKRIQQAYPAQWEQIIDANNQKPPMWLRVNR
HHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEHHH
LHHTREDYLQLMEQAGIAAEPHAEYRDAVRLLAPCAVTDLPGFADGWVTVQDASAQGCVD
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHH
LLDPQDGEQILDLCAAPGGKTTHILEAAPKAHVMAVDIDEQRLKRVKENLQRLRLHAEVK
CCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
QGDGRTPQQWCGDKQFDRILLDAPCSATGVIRRHPDIKWLRRDSDIAELAALQAEIIEAI
CCCCCCHHHHCCCHHHHHHHCCCCCCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHH
WPHLKSGGVMVYATCSILPAENAEQVSAFLQRHADARLVETGNQQQPGRQNLPHPEDGDG
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCC
FFYAKLIKM
EEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA