Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 157372745

GI number: 157372745

Start: 5004312

End: 5005256

Strand: Direct

Name: fmt

Synonym: Spro_4512

Alternate gene names: 157372745

Gene position: 5004312-5005256 (Clockwise)

Preceding gene: 157372744

Following gene: 157372746

Centisome position: 91.84

GC content: 57.35

Gene sequence:

>945_bases
GTGTCTGACTCTTTACGGATTATTTTCGCCGGAACTCCAGACTTCGCAGCGCGTCATCTTGACGCGCTGTTGTCATCTGG
GCACCAGATTGTCGGGGTTTTCACTCAACCCGACCGCCCTGCGGGTCGCGGCAATAAGCTGACGCCCAGCCCAGTCAAGG
TTCTGGCCGAGCAGCACCATTTACCGGTGTTTCAGCCCAAATCACTGCGCCCGGAAGAGAATCAGCACCTGGTTGCCGAT
CTCAACGCCGATGTCATGGTGGTCGTGGCTTATGGTCTGATTCTGCCCAAAGCGGTGTTGGATATGCCGCGACTCGGTTG
CATTAACGTCCATGGTTCACTGCTGCCTCGCTGGCGTGGAGCCGCACCGATCCAACGTTCACTGTGGGCCGGCGATCATG
AAACCGGCGTTACCATCATGCAGATGGACGTGGGTTTGGACACCGGCGATATGATGCACAAGATTGCCTGCCCGATCGAA
GCCGATGACACCAGCGCCAGCCTGTACGATAAACTGGCGCAGCTCGGCCCGCAGGGCATGCTGACCACGTTGCAGCAGAT
GGCTGCAGGTACGGCGAAACGTGAAGTGCAGGATGAATCGCTGGTCACCTATGCCGAAAAGCTGAGCAAAGAAGAAGCCC
GCCTGGACTGGAACCTGCCCGCCGTGCAGCTTGAACGCTGCGTTCGTGCCTTCAACCCGTGGCCAGTCAGTTACTTCACC
ATTGACGATCAACCGGTGAAAGTGTGGCAGGCAACGGTGCTGGCACAAAACGCGGATGCAGAACCTGGCACCATCATCCA
TGCCGACAAGCACGGTATTCAGGTGGCGACCGCCGAAGGCATTCTTAACCTGACTCAACTGCAGCCGGCAGGCAAAAAAC
CCATGTCTGCTCAGGATTTGCTGAACTCACGTCGTGAATGGTTTACGCCGGGCAACCGGTTATAA

Upstream 100 bases:

>100_bases
TCGTCGATTACCTGTCGCCACTCAAGCGCCAGCGTATCCGTCAGAAACTGGAAAAAATGGCCAAGCTTAACGCCCGCGCC
TAACCGATCAGGAAATCAAC

Downstream 100 bases:

>100_bases
ACCGCCCCCACTGCCCGGCCAATAATCGCCGGGCTGTTTCTTTCTTAATGCCGATCGTTATCAGCTTTAGCCTATGAAAA
ACAATTACAATCTCCGCAGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSDSLRIIFAGTPDFAARHLDALLSSGHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHHLPVFQPKSLRPEENQHLVAD
LNADVMVVVAYGLILPKAVLDMPRLGCINVHGSLLPRWRGAAPIQRSLWAGDHETGVTIMQMDVGLDTGDMMHKIACPIE
ADDTSASLYDKLAQLGPQGMLTTLQQMAAGTAKREVQDESLVTYAEKLSKEEARLDWNLPAVQLERCVRAFNPWPVSYFT
IDDQPVKVWQATVLAQNADAEPGTIIHADKHGIQVATAEGILNLTQLQPAGKKPMSAQDLLNSRREWFTPGNRL

Sequences:

>Translated_314_residues
MSDSLRIIFAGTPDFAARHLDALLSSGHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHHLPVFQPKSLRPEENQHLVAD
LNADVMVVVAYGLILPKAVLDMPRLGCINVHGSLLPRWRGAAPIQRSLWAGDHETGVTIMQMDVGLDTGDMMHKIACPIE
ADDTSASLYDKLAQLGPQGMLTTLQQMAAGTAKREVQDESLVTYAEKLSKEEARLDWNLPAVQLERCVRAFNPWPVSYFT
IDDQPVKVWQATVLAQNADAEPGTIIHADKHGIQVATAEGILNLTQLQPAGKKPMSAQDLLNSRREWFTPGNRL
>Mature_313_residues
SDSLRIIFAGTPDFAARHLDALLSSGHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHHLPVFQPKSLRPEENQHLVADL
NADVMVVVAYGLILPKAVLDMPRLGCINVHGSLLPRWRGAAPIQRSLWAGDHETGVTIMQMDVGLDTGDMMHKIACPIEA
DDTSASLYDKLAQLGPQGMLTTLQQMAAGTAKREVQDESLVTYAEKLSKEEARLDWNLPAVQLERCVRAFNPWPVSYFTI
DDQPVKVWQATVLAQNADAEPGTIIHADKHGIQVATAEGILNLTQLQPAGKKPMSAQDLLNSRREWFTPGNRL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=268, Percent_Identity=31.3432835820896, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI238814322, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=31.063829787234, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=80.5732484076433, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1788589, Length=295, Percent_Identity=29.1525423728814, Blast_Score=130, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=273, Percent_Identity=29.3040293040293, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45550868, Length=302, Percent_Identity=30.1324503311258, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=31.6964285714286, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585660, Length=251, Percent_Identity=31.4741035856574, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SERP5 (A8GKG6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480734.1
- ProteinModelPortal:   A8GKG6
- SMR:   A8GKG6
- STRING:   A8GKG6
- GeneID:   5603906
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4512
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   SPRO399741:SPRO_4512-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34435; Mature: 34304

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLRIIFAGTPDFAARHLDALLSSGHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHH
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCC
LPVFQPKSLRPEENQHLVADLNADVMVVVAYGLILPKAVLDMPRLGCINVHGSLLPRWRG
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSLWAGDHETGVTIMQMDVGLDTGDMMHKIACPIEADDTSASLYDKLAQLGPQGM
CCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
LTTLQQMAAGTAKREVQDESLVTYAEKLSKEEARLDWNLPAVQLERCVRAFNPWPVSYFT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
IDDQPVKVWQATVLAQNADAEPGTIIHADKHGIQVATAEGILNLTQLQPAGKKPMSAQDL
ECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCHHHH
LNSRREWFTPGNRL
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SDSLRIIFAGTPDFAARHLDALLSSGHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHH
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCC
LPVFQPKSLRPEENQHLVADLNADVMVVVAYGLILPKAVLDMPRLGCINVHGSLLPRWRG
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSLWAGDHETGVTIMQMDVGLDTGDMMHKIACPIEADDTSASLYDKLAQLGPQGM
CCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
LTTLQQMAAGTAKREVQDESLVTYAEKLSKEEARLDWNLPAVQLERCVRAFNPWPVSYFT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
IDDQPVKVWQATVLAQNADAEPGTIIHADKHGIQVATAEGILNLTQLQPAGKKPMSAQDL
ECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCHHHH
LNSRREWFTPGNRL
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA