Click here to switch to the map view.

The map label for this gene is malE [H]

Identifier: 157372705

GI number: 157372705

Start: 4945782

End: 4946975

Strand: Direct

Name: malE [H]

Synonym: Spro_4472

Alternate gene names: 157372705

Gene position: 4945782-4946975 (Clockwise)

Preceding gene: 157372700

Following gene: 157372706

Centisome position: 90.77

GC content: 56.03

Gene sequence:

>1194_bases
ATGACTCGCAGCTTTACCACCGCCCGCACACTGGTGCTTTCGGCTCTGACCACGTTGGTGCTTTCTTCTTCCGCCTTCGC
CAAGATCGAGGAAGGGAAATTGGTTATCTGGATCAACGGCGATAAGGGCTATAACGGCCTGGCCGAGGTCGGCAAAAAAT
TTGAGAAAGACACCGGTATCAAAGTCACCATCGAGCACCCGGATAAGCTGGAAGAGAAATACCCGCAGGTTGCCGCCACC
GGCGACGGCCCGGATATTATCTTCTGGGCCCATGACCGCTTTGGCGGCTACGCGCAGTCGGGCCTGCTGGCCGAAATCCA
TCCTTCCAAAGCCTTCCAGGACAAACTGTTCCCATTCACCTGGGACGCGGTGCGCTACGACGGCAAGCTGATCGGCTACC
CGATCGCGGTGGAAGCGCTGTCGCTGATTTATAACAAAGACCTGATCAAACAGGCGCCAAAAACCTGGGAAGAGATCCCG
GCGCTGGACAAAGAGCTGCGCGCCAAAGGCAAGAGCGCCATCATGTGGAACCTGCAAGAACCCTACTTCACCTGGCCGAT
TATCGCTGCCGACGGCGGTTATGCCTTCAAGTATGAGAACGGCAAGTACAACATCAAGGACGTAGGCGTGGCCAATACCG
GTTCACAGGCAGGCCTGCAGTTCATCGTCGATTTGGTGAAAAACAAACACATCAACGCCGATACCGATTACTCGATTGCC
GAAGCCGCGTTCAATAAAGGCCAGACCGCGATGACCATCAATGGCCCATGGGCCTGGTCAAACATCGAACAAAGCAAAAT
CAACTACGGCGTGACCCTGCTGCCAACCTTTAAAGGCAAGCCTTCCAAACCTTTCGTCGGCGTACTGACCGCCGGGATCA
ACGCCGCCAGCCCGAACAAAGAGCTGGCGACCGAATTCCTGGAAAACTACCTGCTGACCAACGAAGGGCTGGCGGACGTC
AACAAGGACAAACCGCTGGGCGCTGTAGCGCTGAAGTCCTATCAGGAACAACTGGCGAAAGATCCGAAGATTGCCGCCAC
CATGGAGAACTCACAGAACGGCGAAATCATGCCGAATATCCCGCAGATGAGCGCCTTCTGGTATGCCGAGCGCAGTGCGG
TGATCAACGCCGTCAGCGGCCGCCAGACGGTGAAAGCCGCGCTGGATGACGTACAAACCCGTATCACCAAGTAA

Upstream 100 bases:

>100_bases
GTAGAGGGGGGGAGGATGAGAATGTGTCGCTTTGTGACCCACTATTACCGCGAAACCTCTCTCCTTGATTAACGTGCCCA
TAAAAAAGAAGGATTGGATT

Downstream 100 bases:

>100_bases
CACACTATGTTGTGCGGGGTGCAGACAGGCACCCCGCTGTGGCTTCACAGAAAGGAACGCCCTATGCAATTAGCTCACGC
CGGTTCACCTGCGCGCAAAA

Product: maltose ABC transporter periplasmic protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTRSFTTARTLVLSALTTLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTIEHPDKLEEKYPQVAAT
GDGPDIIFWAHDRFGGYAQSGLLAEIHPSKAFQDKLFPFTWDAVRYDGKLIGYPIAVEALSLIYNKDLIKQAPKTWEEIP
ALDKELRAKGKSAIMWNLQEPYFTWPIIAADGGYAFKYENGKYNIKDVGVANTGSQAGLQFIVDLVKNKHINADTDYSIA
EAAFNKGQTAMTINGPWAWSNIEQSKINYGVTLLPTFKGKPSKPFVGVLTAGINAASPNKELATEFLENYLLTNEGLADV
NKDKPLGAVALKSYQEQLAKDPKIAATMENSQNGEIMPNIPQMSAFWYAERSAVINAVSGRQTVKAALDDVQTRITK

Sequences:

>Translated_397_residues
MTRSFTTARTLVLSALTTLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTIEHPDKLEEKYPQVAAT
GDGPDIIFWAHDRFGGYAQSGLLAEIHPSKAFQDKLFPFTWDAVRYDGKLIGYPIAVEALSLIYNKDLIKQAPKTWEEIP
ALDKELRAKGKSAIMWNLQEPYFTWPIIAADGGYAFKYENGKYNIKDVGVANTGSQAGLQFIVDLVKNKHINADTDYSIA
EAAFNKGQTAMTINGPWAWSNIEQSKINYGVTLLPTFKGKPSKPFVGVLTAGINAASPNKELATEFLENYLLTNEGLADV
NKDKPLGAVALKSYQEQLAKDPKIAATMENSQNGEIMPNIPQMSAFWYAERSAVINAVSGRQTVKAALDDVQTRITK
>Mature_396_residues
TRSFTTARTLVLSALTTLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTIEHPDKLEEKYPQVAATG
DGPDIIFWAHDRFGGYAQSGLLAEIHPSKAFQDKLFPFTWDAVRYDGKLIGYPIAVEALSLIYNKDLIKQAPKTWEEIPA
LDKELRAKGKSAIMWNLQEPYFTWPIIAADGGYAFKYENGKYNIKDVGVANTGSQAGLQFIVDLVKNKHINADTDYSIAE
AAFNKGQTAMTINGPWAWSNIEQSKINYGVTLLPTFKGKPSKPFVGVLTAGINAASPNKELATEFLENYLLTNEGLADVN
KDKPLGAVALKSYQEQLAKDPKIAATMENSQNGEIMPNIPQMSAFWYAERSAVINAVSGRQTVKAALDDVQTRITK

Specific function: Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=392, Percent_Identity=85.969387755102, Blast_Score=704, Evalue=0.0,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43653; Mature: 43522

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSFTTARTLVLSALTTLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGI
CCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCCC
KVTIEHPDKLEEKYPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEIHPSKAFQDKLFPFT
EEEECCCHHHHHHCCCEEECCCCCEEEEEECCCCCCHHHCCEEEEECCCHHHHHCCCCEE
WDAVRYDGKLIGYPIAVEALSLIYNKDLIKQAPKTWEEIPALDKELRAKGKSAIMWNLQE
EHHEEECCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCC
PYFTWPIIAADGGYAFKYENGKYNIKDVGVANTGSQAGLQFIVDLVKNKHINADTDYSIA
CCEEEEEEEECCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
EAAFNKGQTAMTINGPWAWSNIEQSKINYGVTLLPTFKGKPSKPFVGVLTAGINAASPNK
HHHHCCCCEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCH
ELATEFLENYLLTNEGLADVNKDKPLGAVALKSYQEQLAKDPKIAATMENSQNGEIMPNI
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
PQMSAFWYAERSAVINAVSGRQTVKAALDDVQTRITK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRSFTTARTLVLSALTTLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGI
CCCHHHHHHHHHHHHHHHHHCCCHHEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCCC
KVTIEHPDKLEEKYPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEIHPSKAFQDKLFPFT
EEEECCCHHHHHHCCCEEECCCCCEEEEEECCCCCCHHHCCEEEEECCCHHHHHCCCCEE
WDAVRYDGKLIGYPIAVEALSLIYNKDLIKQAPKTWEEIPALDKELRAKGKSAIMWNLQE
EHHEEECCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCC
PYFTWPIIAADGGYAFKYENGKYNIKDVGVANTGSQAGLQFIVDLVKNKHINADTDYSIA
CCEEEEEEEECCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
EAAFNKGQTAMTINGPWAWSNIEQSKINYGVTLLPTFKGKPSKPFVGVLTAGINAASPNK
HHHHCCCCEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCH
ELATEFLENYLLTNEGLADVNKDKPLGAVALKSYQEQLAKDPKIAATMENSQNGEIMPNI
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
PQMSAFWYAERSAVINAVSGRQTVKAALDDVQTRITK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]