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The map label for this gene is rihB [H]

Identifier: 157372700

GI number: 157372700

Start: 4939972

End: 4940955

Strand: Direct

Name: rihB [H]

Synonym: Spro_4467

Alternate gene names: 157372700

Gene position: 4939972-4940955 (Clockwise)

Preceding gene: 157372699

Following gene: 157372705

Centisome position: 90.66

GC content: 61.69

Gene sequence:

>984_bases
ATGCGTTTGATTATTGATTGCGATCCCGGCAACGGCATTCCCGGTGCCAACGTTGACGACGGGCTGGCGCTGGCGCTGGC
GCTGGCAGCCAAACCTCAGCTCTCACTGGAGCTTATCAGCATCGTGGCCGGCAATACGCCGCGCGACGTCGGTTTTGCCG
TGGCCAGCCATTTGTTGCAGCAAACCGGTTATCAGGTACCGGTGGTGGCTGGCGCAACGCGAGCATTGAGCGAAGCGCCA
GAACCCTGGCGCACCCATCTGGATCGGGCGATTACCGATCCGCAGCTAGCCACCCTGTGGCGTAATACGCCTTATCCCCA
AGCCGCCACAATACCCACCCCCAACGCCGCCGTAGCGATCGGCGAGTTGATCTGCAATCACCCCGGCGAGATAACCCTGG
CAGCAATCGGCCCGCTGACCAACGTCGCCCATGCCTTGCAGTTGTACCCGCAGATGGCGCAGGCGGTGAAGGAAATCGTG
ATTATGGGTGGGGTGTTCAACGTCGAGGGTTACATCAAGGACACCAATTTCGGCCTGGATCCGGAAGCGGCCAGGCAGGT
GCTGACCAGCGGTGCCAATATCACGCTCGCTCCGCTGGATGTCACCACGCAAACCATGCTGACGCAGGCTGACCTGGCCC
AAATGACCCAGCCGGATACGCCGCTGTGCCGCTACCTGCGAACCACCACTCAGCCCTGGATCGACTACTCTCGCCATACC
CGCCATCTGCCGGGCTGCTGGATCCATGATGCACTGGTGATTGCCTGGCTGCTGGAACCGCAGTTGGTCACCACGGAGGA
ATATTTTGTCGACGTGGCGCTGGAAGGAGCCATAACGCGCGGCAGCTCCCGTCGCTGGCGACCGGAAAGTCTGCGCCTGA
CGGTCGGCATGCCGGAAGCCGAAGGTCGACCGGTGCGGATAATGCAGCAGGTGGACAATGCGCGCCTGCTGGCGTTGATC
GGTGAAACACTGGCCGACGAATGA

Upstream 100 bases:

>100_bases
TTGGCCACCGGTACCGCGCAGCCAGAACTGCACGAATTCGCCTTCCAGCTGCATATCCGCCGATCGTGCGGCTGCTAGTT
TATTGATTCAGGTAACTGCT

Downstream 100 bases:

>100_bases
AAAAAGGTGCGCCGGGGCGCACCAATCATCAAGCCAGCATCGGCAGCGTCGGGTACTACCCCTTGCCTTTCACGCTGCCA
ATAAACGTTTCACGCGCTGT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRLIIDCDPGNGIPGANVDDGLALALALAAKPQLSLELISIVAGNTPRDVGFAVASHLLQQTGYQVPVVAGATRALSEAP
EPWRTHLDRAITDPQLATLWRNTPYPQAATIPTPNAAVAIGELICNHPGEITLAAIGPLTNVAHALQLYPQMAQAVKEIV
IMGGVFNVEGYIKDTNFGLDPEAARQVLTSGANITLAPLDVTTQTMLTQADLAQMTQPDTPLCRYLRTTTQPWIDYSRHT
RHLPGCWIHDALVIAWLLEPQLVTTEEYFVDVALEGAITRGSSRRWRPESLRLTVGMPEAEGRPVRIMQQVDNARLLALI
GETLADE

Sequences:

>Translated_327_residues
MRLIIDCDPGNGIPGANVDDGLALALALAAKPQLSLELISIVAGNTPRDVGFAVASHLLQQTGYQVPVVAGATRALSEAP
EPWRTHLDRAITDPQLATLWRNTPYPQAATIPTPNAAVAIGELICNHPGEITLAAIGPLTNVAHALQLYPQMAQAVKEIV
IMGGVFNVEGYIKDTNFGLDPEAARQVLTSGANITLAPLDVTTQTMLTQADLAQMTQPDTPLCRYLRTTTQPWIDYSRHT
RHLPGCWIHDALVIAWLLEPQLVTTEEYFVDVALEGAITRGSSRRWRPESLRLTVGMPEAEGRPVRIMQQVDNARLLALI
GETLADE
>Mature_327_residues
MRLIIDCDPGNGIPGANVDDGLALALALAAKPQLSLELISIVAGNTPRDVGFAVASHLLQQTGYQVPVVAGATRALSEAP
EPWRTHLDRAITDPQLATLWRNTPYPQAATIPTPNAAVAIGELICNHPGEITLAAIGPLTNVAHALQLYPQMAQAVKEIV
IMGGVFNVEGYIKDTNFGLDPEAARQVLTSGANITLAPLDVTTQTMLTQADLAQMTQPDTPLCRYLRTTTQPWIDYSRHT
RHLPGCWIHDALVIAWLLEPQLVTTEEYFVDVALEGAITRGSSRRWRPESLRLTVGMPEAEGRPVRIMQQVDNARLLALI
GETLADE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=285, Percent_Identity=29.4736842105263, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786871, Length=328, Percent_Identity=28.6585365853659, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786213, Length=255, Percent_Identity=31.7647058823529, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 35435; Mature: 35435

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIIDCDPGNGIPGANVDDGLALALALAAKPQLSLELISIVAGNTPRDVGFAVASHLLQ
CEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
QTGYQVPVVAGATRALSEAPEPWRTHLDRAITDPQLATLWRNTPYPQAATIPTPNAAVAI
HCCCCEEEECCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHH
GELICNHPGEITLAAIGPLTNVAHALQLYPQMAQAVKEIVIMGGVFNVEGYIKDTNFGLD
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCC
PEAARQVLTSGANITLAPLDVTTQTMLTQADLAQMTQPDTPLCRYLRTTTQPWIDYSRHT
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHCHHHCC
RHLPGCWIHDALVIAWLLEPQLVTTEEYFVDVALEGAITRGSSRRWRPESLRLTVGMPEA
CCCCCHHHHHHHHHHHHHCCCEEECHHHEEEEEEECEEECCCCCCCCCCCEEEEEECCCC
EGRPVRIMQQVDNARLLALIGETLADE
CCCCHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRLIIDCDPGNGIPGANVDDGLALALALAAKPQLSLELISIVAGNTPRDVGFAVASHLLQ
CEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
QTGYQVPVVAGATRALSEAPEPWRTHLDRAITDPQLATLWRNTPYPQAATIPTPNAAVAI
HCCCCEEEECCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHH
GELICNHPGEITLAAIGPLTNVAHALQLYPQMAQAVKEIVIMGGVFNVEGYIKDTNFGLD
HHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCC
PEAARQVLTSGANITLAPLDVTTQTMLTQADLAQMTQPDTPLCRYLRTTTQPWIDYSRHT
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHCHHHCC
RHLPGCWIHDALVIAWLLEPQLVTTEEYFVDVALEGAITRGSSRRWRPESLRLTVGMPEA
CCCCCHHHHHHHHHHHHHCCCEEECHHHEEEEEEECEEECCCCCCCCCCCEEEEEECCCC
EGRPVRIMQQVDNARLLALIGETLADE
CCCCHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA