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The map label for this gene is dhaM [H]

Identifier: 157372503

GI number: 157372503

Start: 4735023

End: 4736456

Strand: Reverse

Name: dhaM [H]

Synonym: Spro_4270

Alternate gene names: 157372503

Gene position: 4736456-4735023 (Counterclockwise)

Preceding gene: 157372504

Following gene: 157372500

Centisome position: 86.93

GC content: 62.48

Gene sequence:

>1434_bases
ATGATCAATATCGTTGTAGTTTCACACAGCGCGCTGTTGGCGCGCGGGGTTGAAGAGCTGGCGTTGCAGATGATGCGTGG
CGATGGCTGCAAACTGGTGCTGGCGGCGGGGGTCGATGATGCGGAGCACCCGATAGGCACCGACGCTGTCAAAGTGATGG
AGGCGATTGAGTCGGTGGCTGACGGTGCGGGCATTGTGGTGCTGATGGATTTGGGCAGTGCGCTGCTGAGCGCCGAAACC
GCCATCGATCTGCTGGATCCGTCGTTGGCCGCCAAAGTCCGGCTGTGCTCGGCACCGCTGGTCGAAGGGACGCTGGCCGC
GGTGGTGGCTGCCAATGCCGGAGCCGGGCTGGAACAGGTACTGGCCGAGGCGCAGGGCGCATTGCAGGCCAAACAGGCGC
AGTTGGGGGAAGCCACCGCGCCGGTTGCGCAAAGCGTTGAATTGCCGCTTGCTCAGGGGAAAAGTGTCAGTTGGACGGTA
CAGAACCCGCATGGCCTGCACGCCCGACCGGCGGCGCGGCTGGCGGAAGCGTTGGCCCCCTTTGATGCCGAGCTGGTGCT
GGAAAAACAGGGCCAGTGCGCCAATCCGCGTAGTCTTAACCAGATTGCGCTTTTGCAGGTGCGCCATGGCGACACCATTC
GGCTGATTGCGGACGGACCACAGGCCGAACAGGCGCTGGCGGCGTTTAGCGCACTGGCGGAGCAGCACTTTGGCGAAACG
GTCTCCGAGCAGAGCCTGCCGTCGCTGCACGGTATTCCGGTGGCGGAGAGCGTGACCAGTGGCCCGGTGTGGCAGGCGCT
GAGCTTCTGGCCGACAGTGGTCGAGCGCACTATTGGCGCTGACGACGTGCTGAACGAACAACAGCGGCTACGCCAGGCGT
TGCAGCTCACGCTGAGCGATTTGAACCGGTTGGCGGAGCGCACTGGCTCATTGATTGGCAAACCGCAGGCGGCCATATTC
GGCGCCCACAGCATGCTGCTGGACGATCCAGATCTGCAGCAGGCGGCCTATACCCGCATCGCCCGCCAGCAATGCAGCGC
CGAGCAGGCATGGCGGCAAGAGATGGAAGCGATTGCCGGGGAGTATCGTGAACTGGATGATGAATATATGCGGGCGCGGG
AATTGGACGTGCGCGACATGCTGCGTCGCACCCTGAGTCATCTGCAACAGCAGCCGTTACCGGTGATTACGCTAACCGCG
CCGTCCATTCTGGTGATGGATGAACTGATGCCGTCTGACGTGGTGATGCTTGATCGTCGTCTGGTGCTCGGCATCTGCTT
AAGCGGCGGTAACGCACTGTCGCACAGCGCCATTTTGGCGAAGGCGATGGGCATGCCGATGGTGGTGGGCATGAACGATT
GCATCAGCAAAACCCGCAACGGTCAGAAAGCGATGCTGGATGCGGCGCGCGGTGTGTTGCAACTCAGCCACTGA

Upstream 100 bases:

>100_bases
TGGGTGAGCGCAGCATCGGCCATCAGGATCCGGGTGCCACCTCGGTGATGCTGATGATGAAAACGTTGGCAGCGGTCGCC
GGCCAATAGCAGGAGGCGAG

Downstream 100 bases:

>100_bases
CGGCATGCCCTCAGGCGCGCAGCTTAAACTGGCGAATATCAATCTGATGCTGTTTGATTTTTCGCCACAGCGTGGTGCGA
CCAATGCCCAGCAGCTGCGC

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MINIVVVSHSALLARGVEELALQMMRGDGCKLVLAAGVDDAEHPIGTDAVKVMEAIESVADGAGIVVLMDLGSALLSAET
AIDLLDPSLAAKVRLCSAPLVEGTLAAVVAANAGAGLEQVLAEAQGALQAKQAQLGEATAPVAQSVELPLAQGKSVSWTV
QNPHGLHARPAARLAEALAPFDAELVLEKQGQCANPRSLNQIALLQVRHGDTIRLIADGPQAEQALAAFSALAEQHFGET
VSEQSLPSLHGIPVAESVTSGPVWQALSFWPTVVERTIGADDVLNEQQRLRQALQLTLSDLNRLAERTGSLIGKPQAAIF
GAHSMLLDDPDLQQAAYTRIARQQCSAEQAWRQEMEAIAGEYRELDDEYMRARELDVRDMLRRTLSHLQQQPLPVITLTA
PSILVMDELMPSDVVMLDRRLVLGICLSGGNALSHSAILAKAMGMPMVVGMNDCISKTRNGQKAMLDAARGVLQLSH

Sequences:

>Translated_477_residues
MINIVVVSHSALLARGVEELALQMMRGDGCKLVLAAGVDDAEHPIGTDAVKVMEAIESVADGAGIVVLMDLGSALLSAET
AIDLLDPSLAAKVRLCSAPLVEGTLAAVVAANAGAGLEQVLAEAQGALQAKQAQLGEATAPVAQSVELPLAQGKSVSWTV
QNPHGLHARPAARLAEALAPFDAELVLEKQGQCANPRSLNQIALLQVRHGDTIRLIADGPQAEQALAAFSALAEQHFGET
VSEQSLPSLHGIPVAESVTSGPVWQALSFWPTVVERTIGADDVLNEQQRLRQALQLTLSDLNRLAERTGSLIGKPQAAIF
GAHSMLLDDPDLQQAAYTRIARQQCSAEQAWRQEMEAIAGEYRELDDEYMRARELDVRDMLRRTLSHLQQQPLPVITLTA
PSILVMDELMPSDVVMLDRRLVLGICLSGGNALSHSAILAKAMGMPMVVGMNDCISKTRNGQKAMLDAARGVLQLSH
>Mature_477_residues
MINIVVVSHSALLARGVEELALQMMRGDGCKLVLAAGVDDAEHPIGTDAVKVMEAIESVADGAGIVVLMDLGSALLSAET
AIDLLDPSLAAKVRLCSAPLVEGTLAAVVAANAGAGLEQVLAEAQGALQAKQAQLGEATAPVAQSVELPLAQGKSVSWTV
QNPHGLHARPAARLAEALAPFDAELVLEKQGQCANPRSLNQIALLQVRHGDTIRLIADGPQAEQALAAFSALAEQHFGET
VSEQSLPSLHGIPVAESVTSGPVWQALSFWPTVVERTIGADDVLNEQQRLRQALQLTLSDLNRLAERTGSLIGKPQAAIF
GAHSMLLDDPDLQQAAYTRIARQQCSAEQAWRQEMEAIAGEYRELDDEYMRARELDVRDMLRRTLSHLQQQPLPVITLTA
PSILVMDELMPSDVVMLDRRLVLGICLSGGNALSHSAILAKAMGMPMVVGMNDCISKTRNGQKAMLDAARGVLQLSH

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG3412

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=451, Percent_Identity=48.780487804878, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788756, Length=196, Percent_Identity=32.6530612244898, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788726, Length=344, Percent_Identity=26.453488372093, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994992, Length=321, Percent_Identity=26.4797507788162, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789193, Length=232, Percent_Identity=28.448275862069, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 50738; Mature: 50738

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIVVVSHSALLARGVEELALQMMRGDGCKLVLAAGVDDAEHPIGTDAVKVMEAIESVA
CEEEEEECCHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DGAGIVVLMDLGSALLSAETAIDLLDPSLAAKVRLCSAPLVEGTLAAVVAANAGAGLEQV
CCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
LAEAQGALQAKQAQLGEATAPVAQSVELPLAQGKSVSWTVQNPHGLHARPAARLAEALAP
HHHHHHHHHHHHHHHCCHHHHHHHHCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
FDAELVLEKQGQCANPRSLNQIALLQVRHGDTIRLIADGPQAEQALAAFSALAEQHFGET
CCHHHHEECCCCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCH
VSEQSLPSLHGIPVAESVTSGPVWQALSFWPTVVERTIGADDVLNEQQRLRQALQLTLSD
HHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LNRLAERTGSLIGKPQAAIFGAHSMLLDDPDLQQAAYTRIARQQCSAEQAWRQEMEAIAG
HHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYRELDDEYMRARELDVRDMLRRTLSHLQQQPLPVITLTAPSILVMDELMPSDVVMLDRR
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHH
LVLGICLSGGNALSHSAILAKAMGMPMVVGMNDCISKTRNGQKAMLDAARGVLQLSH
HHHHEEECCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINIVVVSHSALLARGVEELALQMMRGDGCKLVLAAGVDDAEHPIGTDAVKVMEAIESVA
CEEEEEECCHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DGAGIVVLMDLGSALLSAETAIDLLDPSLAAKVRLCSAPLVEGTLAAVVAANAGAGLEQV
CCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
LAEAQGALQAKQAQLGEATAPVAQSVELPLAQGKSVSWTVQNPHGLHARPAARLAEALAP
HHHHHHHHHHHHHHHCCHHHHHHHHCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
FDAELVLEKQGQCANPRSLNQIALLQVRHGDTIRLIADGPQAEQALAAFSALAEQHFGET
CCHHHHEECCCCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCH
VSEQSLPSLHGIPVAESVTSGPVWQALSFWPTVVERTIGADDVLNEQQRLRQALQLTLSD
HHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LNRLAERTGSLIGKPQAAIFGAHSMLLDDPDLQQAAYTRIARQQCSAEQAWRQEMEAIAG
HHHHHHHHHHHCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYRELDDEYMRARELDVRDMLRRTLSHLQQQPLPVITLTAPSILVMDELMPSDVVMLDRR
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHH
LVLGICLSGGNALSHSAILAKAMGMPMVVGMNDCISKTRNGQKAMLDAARGVLQLSH
HHHHEEECCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]