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The map label for this gene is nudF [H]

Identifier: 157372500

GI number: 157372500

Start: 4730507

End: 4731139

Strand: Reverse

Name: nudF [H]

Synonym: Spro_4267

Alternate gene names: 157372500

Gene position: 4731139-4730507 (Counterclockwise)

Preceding gene: 157372503

Following gene: 157372499

Centisome position: 86.83

GC content: 54.98

Gene sequence:

>633_bases
ATGAGCCACTCGCTTCCGTCGCCCGTTACTCTTGATGAAAATGATGTAGAAATTATTGCACGGGAGACGCTTTACCGTGG
TTTTTTTTCACTCAATTTATATCGTTTCCGCCACCGCCTGTTCAACGGTGACATGAGCCCAGAAATTAAACGTGAAATTT
TTGAGCGTGGGCATGCCGCGGTGTTACTTCCCTATGACCCGGTGCGTGACGAGGTGGTGCTGATTGAACAACTGCGGATC
GCCGCGGTCGATACCAGTAACTCACCCTGGCTACTGGAAATGGTGGCGGGTATGATCGAAACCGGCGAAAGCGTTGAAGA
CGTGTGCCGCCGTGAAGCGCAGGAAGAGGCCGGCGTCGATGTCGGGCGGTGCAAGCCGGTGCTGAGCTACCTGGCCAGCC
CCGGCGGTACCAGCGAGCGCCTGTCGATTATGGTGGGCGAAGTGGACGCGACTACCGCAGTTGGCATCCATGGACTGGAA
GAAGAGCATGAAGATATCCGTGTCCATGTGGTCAGCCGCGAACAGGCTTACGGCTGGGTTGAAGAGGGCGCGATTGATAA
CGCGGCATCAGTGATTGCATTGCAGTGGCTGGCGTTGCACCATGAATCGTTAAGAAAAGAGTGGGCCAATTAA

Upstream 100 bases:

>100_bases
ATGGCTCACAATCTATCATAAAGCCGCGGAAGATTATCCCGCCGTTTCTGTCATCAAAACTGTTTATTTTATCGGCATAC
TCATGCAGGAGAATTCACTT

Downstream 100 bases:

>100_bases
TGAAACAGCGCTATACCCCTGATTTCCCCGAAATGATGAGATTGTGCGAAACCAACTTCGCGCAATTGCGCCGCTTGTTG
CCGCGCAATGACGAAGTGGG

Product: ADP-ribose pyrophosphatase NudF

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSHSLPSPVTLDENDVEIIARETLYRGFFSLNLYRFRHRLFNGDMSPEIKREIFERGHAAVLLPYDPVRDEVVLIEQLRI
AAVDTSNSPWLLEMVAGMIETGESVEDVCRREAQEEAGVDVGRCKPVLSYLASPGGTSERLSIMVGEVDATTAVGIHGLE
EEHEDIRVHVVSREQAYGWVEEGAIDNAASVIALQWLALHHESLRKEWAN

Sequences:

>Translated_210_residues
MSHSLPSPVTLDENDVEIIARETLYRGFFSLNLYRFRHRLFNGDMSPEIKREIFERGHAAVLLPYDPVRDEVVLIEQLRI
AAVDTSNSPWLLEMVAGMIETGESVEDVCRREAQEEAGVDVGRCKPVLSYLASPGGTSERLSIMVGEVDATTAVGIHGLE
EEHEDIRVHVVSREQAYGWVEEGAIDNAASVIALQWLALHHESLRKEWAN
>Mature_209_residues
SHSLPSPVTLDENDVEIIARETLYRGFFSLNLYRFRHRLFNGDMSPEIKREIFERGHAAVLLPYDPVRDEVVLIEQLRIA
AVDTSNSPWLLEMVAGMIETGESVEDVCRREAQEEAGVDVGRCKPVLSYLASPGGTSERLSIMVGEVDATTAVGIHGLEE
EHEDIRVHVVSREQAYGWVEEGAIDNAASVIALQWLALHHESLRKEWAN

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=202, Percent_Identity=83.6633663366337, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788810, Length=189, Percent_Identity=31.2169312169312, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23550; Mature: 23419

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSLPSPVTLDENDVEIIARETLYRGFFSLNLYRFRHRLFNGDMSPEIKREIFERGHAA
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VLLPYDPVRDEVVLIEQLRIAAVDTSNSPWLLEMVAGMIETGESVEDVCRREAQEEAGVD
EEECCCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VGRCKPVLSYLASPGGTSERLSIMVGEVDATTAVGIHGLEEEHEDIRVHVVSREQAYGWV
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEEEECHHHCCCH
EEGAIDNAASVIALQWLALHHESLRKEWAN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHSLPSPVTLDENDVEIIARETLYRGFFSLNLYRFRHRLFNGDMSPEIKREIFERGHAA
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEE
VLLPYDPVRDEVVLIEQLRIAAVDTSNSPWLLEMVAGMIETGESVEDVCRREAQEEAGVD
EEECCCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VGRCKPVLSYLASPGGTSERLSIMVGEVDATTAVGIHGLEEEHEDIRVHVVSREQAYGWV
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCEEEEEEECHHHCCCH
EEGAIDNAASVIALQWLALHHESLRKEWAN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]