Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 157372495

GI number: 157372495

Start: 4725710

End: 4726606

Strand: Reverse

Name: yafC [H]

Synonym: Spro_4262

Alternate gene names: 157372495

Gene position: 4726606-4725710 (Counterclockwise)

Preceding gene: 157372496

Following gene: 157372492

Centisome position: 86.74

GC content: 58.08

Gene sequence:

>897_bases
ATGAAAATCACCCTCGAAGAGTTACTGGCGTTTACCACCGTTGTTGATAGCGGTTCCATTACTGCCGCCGCCGAACAACT
TGGCCAGACCACCTCCGGCATCAGCCGTGCCCTCAGCCGGTTGGAAAAAAAGCTCGATACGACCCTGATGCGCCGCACCA
CGCGCCGGTTGGAGCTGACCGAAGAAGGCCAAAGTTTTCTCGGCCATGCGCGTGAAATTATCAACTCGGTGGAGAATGCT
GAAGAGCAGATGGCGCTGCGCAGGCTGATCCCGGCCGGCAGGCTGCGGGTCAATGCCGCCGCCCCCTTTATGGAACACGT
GATTGTGCCGCTGGTGGCGGGCTTTCGCCAAAGCTACCCGCAAATTGAGCTGGAACTGAATACCGATAACCTGATCATCG
ATCTGCTGGAGAAACGCACCGATATTGCCATTCGCATCGGCGCGCTGCGGGATTCGACCATTCATGCGCGCCTGCTGGGG
GCCAGTCGTTTACGTATTCTCGCCAGCCCGGATTACCTCGCACGTTACGGTACACCGGCCAGCGTCGAAGCGCTGCGAGA
TCACAGCCTGCTGGGCTTCACCCAACCGGAGTCACTCAATCTGTGGCCGCTGCGTTATCAGCAGGCACAGCATTTTGCTA
TCACGCCGACAATTTCGGCATCCAGCGGCGAAACCTTGCGACAGCTGGCGTTGCGGGGTGAAGGGATTGTGCAACTGGCT
GATTTTATGACCCGTGACGATCAGCGTAGCGGGCGGCTGGTGCCGTTGCTGGAGCAGGATACGTTGGATGTGCGCCAGCC
GATTAATGCGGTCTATTACCGCAATACCCAGCTGGCGGCGCGTATTACCTGTTTCCTGGACTACGTCAGCGCGCATATTG
ATGCGCAGATGCTTTAG

Upstream 100 bases:

>100_bases
CTGACAGACCAGGCGAAAGACTGACAGACCCGGCTTCCCCGCCGGGTCTTCTTCCCGCAGGGGGACCGCGTGCGGCACCC
GACCACACTGGGCGGAATCC

Downstream 100 bases:

>100_bases
AGATCGACAGGCATTACAGTGCGTTACCCAGTACGTGAATGGTCACGCCCAGTACGCTGTCCTTGATGGTGTCACGATGC
CGATGCATGTGAGCGATATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKITLEELLAFTTVVDSGSITAAAEQLGQTTSGISRALSRLEKKLDTTLMRRTTRRLELTEEGQSFLGHAREIINSVENA
EEQMALRRLIPAGRLRVNAAAPFMEHVIVPLVAGFRQSYPQIELELNTDNLIIDLLEKRTDIAIRIGALRDSTIHARLLG
ASRLRILASPDYLARYGTPASVEALRDHSLLGFTQPESLNLWPLRYQQAQHFAITPTISASSGETLRQLALRGEGIVQLA
DFMTRDDQRSGRLVPLLEQDTLDVRQPINAVYYRNTQLAARITCFLDYVSAHIDAQML

Sequences:

>Translated_298_residues
MKITLEELLAFTTVVDSGSITAAAEQLGQTTSGISRALSRLEKKLDTTLMRRTTRRLELTEEGQSFLGHAREIINSVENA
EEQMALRRLIPAGRLRVNAAAPFMEHVIVPLVAGFRQSYPQIELELNTDNLIIDLLEKRTDIAIRIGALRDSTIHARLLG
ASRLRILASPDYLARYGTPASVEALRDHSLLGFTQPESLNLWPLRYQQAQHFAITPTISASSGETLRQLALRGEGIVQLA
DFMTRDDQRSGRLVPLLEQDTLDVRQPINAVYYRNTQLAARITCFLDYVSAHIDAQML
>Mature_298_residues
MKITLEELLAFTTVVDSGSITAAAEQLGQTTSGISRALSRLEKKLDTTLMRRTTRRLELTEEGQSFLGHAREIINSVENA
EEQMALRRLIPAGRLRVNAAAPFMEHVIVPLVAGFRQSYPQIELELNTDNLIIDLLEKRTDIAIRIGALRDSTIHARLLG
ASRLRILASPDYLARYGTPASVEALRDHSLLGFTQPESLNLWPLRYQQAQHFAITPTISASSGETLRQLALRGEGIVQLA
DFMTRDDQRSGRLVPLLEQDTLDVRQPINAVYYRNTQLAARITCFLDYVSAHIDAQML

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=44.0273037542662, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=28.9115646258503, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=28.3216783216783, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.3737024221453, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=29.1095890410959, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789440, Length=279, Percent_Identity=29.3906810035842, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=25.6944444444444, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786508, Length=266, Percent_Identity=30.0751879699248, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082132, Length=169, Percent_Identity=27.810650887574, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787879, Length=270, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786448, Length=184, Percent_Identity=29.3478260869565, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788481, Length=189, Percent_Identity=30.6878306878307, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788706, Length=223, Percent_Identity=23.3183856502242, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=132, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33318; Mature: 33318

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITLEELLAFTTVVDSGSITAAAEQLGQTTSGISRALSRLEKKLDTTLMRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGQSFLGHAREIINSVENAEEQMALRRLIPAGRLRVNAAAPFMEHVIVPLVAGFRQSYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCC
QIELELNTDNLIIDLLEKRTDIAIRIGALRDSTIHARLLGASRLRILASPDYLARYGTPA
EEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCHHHEECCCHHEEEEECCHHHHHCCCCH
SVEALRDHSLLGFTQPESLNLWPLRYQQAQHFAITPTISASSGETLRQLALRGEGIVQLA
HHHHHHCCCEECCCCCCCCCCCEEEHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHH
DFMTRDDQRSGRLVPLLEQDTLDVRQPINAVYYRNTQLAARITCFLDYVSAHIDAQML
HHHCCCCCCCCCEEEEECCCCHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKITLEELLAFTTVVDSGSITAAAEQLGQTTSGISRALSRLEKKLDTTLMRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGQSFLGHAREIINSVENAEEQMALRRLIPAGRLRVNAAAPFMEHVIVPLVAGFRQSYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCC
QIELELNTDNLIIDLLEKRTDIAIRIGALRDSTIHARLLGASRLRILASPDYLARYGTPA
EEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCHHHEECCCHHEEEEECCHHHHHCCCCH
SVEALRDHSLLGFTQPESLNLWPLRYQQAQHFAITPTISASSGETLRQLALRGEGIVQLA
HHHHHHCCCEECCCCCCCCCCCEEEHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHH
DFMTRDDQRSGRLVPLLEQDTLDVRQPINAVYYRNTQLAARITCFLDYVSAHIDAQML
HHHCCCCCCCCCEEEEECCCCHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]